Effects of sialidase knockout and complementation on virulence of Mycoplasma gallisepticum

• Published in: Veterinary microbiology Vol. 157; no. 1-2; pp. 91 - 95
• Main Authors: May, Meghan, Szczepanek, Steven M., Frasca, Salvatore, Gates, Amy E., Demcovitz, Dina L., Moneypenny, Craig G., Brown, Daniel R., Geary, Steven J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25.05.2012; Elsevier
• Subjects: Animals; Bacteriology; Biological and medical sciences; Chickens - microbiology; Chronic respiratory disease; Fundamental and applied biological sciences. Psychology; Gene Knockout Techniques; Genetic Complementation Test; Infection study; Microbiology; Miscellaneous; Mutagenesis, Insertional; Mycoplasma gallisepticum; Mycoplasma gallisepticum - enzymology; Mycoplasma gallisepticum - genetics; Mycoplasma gallisepticum - pathogenicity; Mycoplasma Infections - microbiology; Mycoplasma Infections - pathology; Mycoplasma Infections - veterinary; Mycoplasma synoviae; Mycoplasma synoviae - enzymology; Neuraminidase; Neuraminidase - genetics; Sialidase; Virulence; Infection study; Virulence; Sialidase; Neuraminidase; Chronic respiratory disease; Mycoplasma gallisepticum; Enzyme; Chronic disease; Respiratory disease; Mycoplasma galiisepticum; Mycoplasmataceae; Glycosylases; Mollicutes; Mycoplasmatales; Glycosidases; Exo-α-sialidase; Bacteria; Hydrolases; Complementation; Veterinary

Reannotation of the pathogenic Mycoplasma gallisepticum strain Rlow genome identified the hypothetical gene MGA_0329 as a homolog of the sialidase gene MS53_0199 of Mycoplasma synoviae strain MS53. Potent sialidase activity was subsequently quantitated in several M. gallisepticum strains. Because si...