G3139, an Anti-Bcl-2 Antisense Oligomer That Binds Heparin-Binding Growth Factors and Collagen I, Alters In vitro Endothelial Cell Growth and Tubular Morphogenesis

• Published in: Clinical cancer research Vol. 15; no. 8; pp. 2797 - 2807
• Main Authors: STEIN, C. A, SIJIAN WU, VOSKRESENSKIY, Anatoliy M, ZHOU, Jin-Feng, SHIN, Joongho, MILLER, Paul, SOULEIMANIAN, Naira, BENIMETSKAYA, Luba
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.04.2009
• Subjects: Animals; Antineoplastic agents; Biological and medical sciences; Cell Proliferation - drug effects; Collagen Type I - metabolism; Cytoskeleton - drug effects; Cytoskeleton - metabolism; endothelial; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Fibroblast Growth Factor 2 - drug effects; Fibroblast Growth Factor 2 - metabolism; G3139; Humans; Medical sciences; oligonucleotide; Oligonucleotides, Antisense - genetics; Oligonucleotides, Antisense - metabolism; Oligonucleotides, Antisense - pharmacology; Pharmacology. Drug treatments; Platelet-Derived Growth Factor - metabolism; Proto-Oncogene Proteins c-bcl-2 - antagonists & inhibitors; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-sis; Rats; Receptor, Fibroblast Growth Factor, Type 1 - drug effects; Receptor, Fibroblast Growth Factor, Type 1 - metabolism; Thionucleotides - genetics; Thionucleotides - metabolism; Thionucleotides - pharmacology; Vascular Endothelial Growth Factor A - metabolism; Morphogenesis; Cell proliferation; Fibroblast growth factor; Endothelial cell; Collagen type I; C-Onc gene; Oligomer; In vitro; Midkine; Protooncogene

Purpose: We examined the effects of G3139 on the interaction of heparin-binding proteins [e.g., fibroblast growth factor 2 (FGF2) and collagen I] with endothelial cells. G3139 is an 18-mer phosphorothioate oligonucleotide targeted to the initiation codon region of the Bcl-2 mRNA. A randomized, prosp...