Synthesis of silver nanoparticles using marine macroalgae Padina sp. and its antibacterial activity towards pathogenic bacteria

• Published in: Beni-Suef University journal of basic and applied sciences Vol. 9; no. 1; pp. 1 - 15
• Main Authors: Bhuyar, Prakash, Rahim, Mohd Hasbi Ab, Sundararaju, Sathyavathi, Ramaraj, Rameshprabu, Maniam, Gaanty Pragas, Govindan, Natanamurugaraj
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 09.01.2020; Springer Nature B.V; SpringerOpen
• Subjects: Absorption spectra; Algae; Antibacterial activity; Antimicrobial activity; Bacteria; Biosynthesis; Composite materials; Drug resistance; Energy dispersive X ray spectroscopy; Field emission microscopy; Food sources; Fourier transforms; Gram-negative bacteria; Gram-positive bacteria; Infrared spectrophotometers; Infrared spectroscopy; Marine algae; Measurement techniques; Medical equipment; Medicine; Medicine & Public Health; Moisture content; Morphology; Nanoparticles; Nanostructured materials; Nitrates; Padina; Padina sp; Polyethylene; Pseudomonas aeruginosa; Scanning electron microscopy; Seaweeds; Silver; Silver nanoparticles; Synthesis; X-ray spectroscopy; United States > US; Antimicrobial activity; Marine algae; sp; Silver nanoparticles
• ISSN: 2314-8543; 2314-8535; 2314-8543
• DOI: 10.1186/s43088-019-0031-y

Background Marine algae used as a food source for ocean life and range in color from red to green to brown grow along rocky shorelines around the world. The synthesis of silver nanoparticles by marine alga Padina sp. and its characterization were fulfilled by using UV-visible spectrophotometer, Fourier transform infrared spectroscopy, scanning electron microscopy and field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Results UV-visible absorption spectrum revealed that the formation of Ag nanoparticles was increased by the addition of marine algae and the spectral peak observed between a wavelength of ~ 420 nm and 445 nm. In addition, SEM and FESEM images examined the surface morphology and the size of the synthesized NPs was relatively uniform in size ~ 25–60 nm. Energy-dispersive X-ray spectroscopy analysis confirmed the purity of Ag NPs with atomic percentage of 48.34% Ag. The synthesized Ag NPs showed highly potent antibacterial activity. The Staphylococcus aureus and Pseudomonas aeruginosa were found to be more susceptible to silver nanoparticles by forming 15.17 ± 0.58 mm and 13.33 ± 0.76 mm of diameter of the inhibition zone, respectively. Conclusions The study suggested that marine alga Padina sp. could be an alternative source for the production of Ag nanoparticles and are efficient antimicrobial compounds against both gram-negative and gram-positive bacteria which can be a promising material against infectious bacteria.