Involvement of the G3P shuttle and β-oxidation pathway in the control of TAG synthesis and lipid accumulation in Yarrowia lipolytica

• Published in: Metabolic engineering Vol. 13; no. 5; pp. 482 - 491
• Main Authors: Dulermo, Thierry, Nicaud, Jean-Marc
• Format: Journal Article
• Language: English
• Published: Belgium Elsevier Inc 01.09.2011; Elsevier
• Subjects: Agricultural sciences; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; beta oxidation; carboxylic ester hydrolases; diacylglycerol acyltransferase; fatty acid composition; fatty acids; free fatty acids; gene overexpression; genes; genetic background; glycerol; Glycerol - metabolism; Glycerol-3-phosphate; Glycerol-3-phosphate shuttle; Glycerophosphates - genetics; Glycerophosphates - metabolism; homeostasis; Life Sciences; Lipids; Mutation; Oxidation-Reduction; transcription (genetics); Triacylglyceride; triacylglycerols; Triglycerides - biosynthesis; Triglycerides - genetics; Yarrowia - genetics; Yarrowia - metabolism; Yarrowia lipolytica; yeasts; FFA; Glycerol-3-phosphate shuttle; SE; Glycerol-3-phosphate; G3P; TGL; Lipids; Triacylglyceride; FA; TAG; Glycerol metabolism; Yarrowia lipolytica; GLYCEROL-3-PHOSPHATE DEHYDROGENASE; ASPERGILLUS-NIDULANS; FATTY-ACIDS; SACCHAROMYCES-CEREVISIAE; ALDO-KETO REDUCTASE; YEAST; GENE; GLYCEROL 3-PHOSPHATE DEHYDROGENASE; PURIFICATION; EXPRESSION

The oleaginous yeast Yarrowia lipolytica can accumulate up to 38% of its dry weight (DW) as lipids. Factors involved in lipid accumulation, particularly triglycerides, are not well identified. Using different mutations in the glycerol-3-phosphate (G3P) shuttle pathway ( Δgut2 affecting the anabolic...