Analysis and solution of false-positives when testing CVA16 sera using an antibody assay against the EV71 virus

• Published in: Virus research Vol. 176; no. 1-2; pp. 33 - 36
• Main Authors: Wang, Changbing, You, Aiping, Tian, Xingui, Zhao, Mingqi, Chen, Yi, Lin, Tao, Zheng, Jianbin, Xiao, Misi, Zhang, Yingying, Kuang, Lu, Zhou, Zhenwen, Zhu, Bing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2013
• Subjects: antibodies; Antibodies, Viral - blood; antigens; Antigens, Viral; Child; Child, Preschool; Coxsackievirus; cross reaction; Cross Reactions; Cross-reactivity; CVA16; Diagnostic Tests, Routine - methods; early diagnosis; Enterovirus; Enterovirus - immunology; Enterovirus A; Enterovirus A, Human - immunology; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; EV71; False Positive Reactions; foot-and-mouth disease; hand, foot and mouth disease; Hand, Foot and Mouth Disease - diagnosis; Hand, Foot and Mouth Disease - virology; Humans; immunoblotting; immunoglobulin M; Immunoglobulin M - blood; methods; patients; peptides; Sensitivity and Specificity; virion; Virology - methods; viruses; VP1; VP3; PBS; DAB; Cross-reactivity; HRP; TMB; EV71; CVA16; TCID50; SDS-PAGE; HFMD; OD; RT-PCR; FBS; VP1; CSF; VP3; ELISA; Enterovirus 71; sodium dodecyl sulfate polyacrylamide gel electrophoresis; 3,3′-diaminobenzidine; phosphate-buffered saline; 3,3′,5,5′-tetramethylbenzidine; horseradish peroxidase; rhabdomyosarcoma (RD) cells, fetal bovine serum; real-time polymerase chain reaction; TCID; hand, foot and mouth disease; cerebrospinal fluid; enzyme-linked immunosorbent assay; 50% tissue culture infective dose; optical density; Coxsackievirus A16

•The VP3 is the cross-reacting antigen in the EV71 IgM-capture ELISA with CVA16 serum.•The VP1 detection method is as sensitive as the virus-antibody detection method.•The VP1 detection method can resolve the false-positive problem. Hand, foot and mouth disease (HFMD) in humans is caused mainly by E...