Biowire platform for maturation of human pluripotent stem cell-derived cardiomyocytes

[Display omitted] •Biowire is a hybrid method to mature hPSC-derived cardiac tissues.•The design and construction of biowire is described.•The biowire platform can produce aligned cardiac tissues with frequent striations.•Electrically stimulated biowires show changes consistent with cardiomyocyte ma...

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Bibliographic Details
Published inMethods (San Diego, Calif.) Vol. 101; pp. 21 - 26
Main Authors Sun, Xuetao, Nunes, Sara S.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.05.2016
Subjects
Biomimetic cues
Cardiomyocyte
Cell Culture Techniques
Cell Differentiation
Electric Stimulation
Humans
Maturation
Myocytes, Cardiac - physiology
Pluripotent Stem Cells - physiology
Stem cells
Tissue engineering
Biomimetic cues
Maturation
Tissue engineering
Cardiomyocyte
Stem cells
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Summary:[Display omitted] •Biowire is a hybrid method to mature hPSC-derived cardiac tissues.•The design and construction of biowire is described.•The biowire platform can produce aligned cardiac tissues with frequent striations.•Electrically stimulated biowires show changes consistent with cardiomyocyte maturation.•These changes are dependent on the stimulation rate. Human pluripotent stem cells (hPSCs)-derived cardiomyocytes (hPSC-CMs) represent a potential indefinite cell supply for cardiac tissue engineering and possibly regenerative medicine applications. However, these cells are immature compared with adult ventricular cardiomyocytes. In order to overcome this limitation, an engineered platform, called biowire, was devised to provide cultured cardiomyocytes important biomimetic cues present during embryo development, such as three-dimensional cell culture, extracellular matrix composition, soluble factors and pacing through electrical stimulation, to induce the maturation of hPSC-CMs in vitro.
Bibliography:ObjectType-Article-2
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ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2015.11.005