Azlactone-reactive polymer supports for immobilizing synthetically useful enzymes. II. Important preliminary hydrogen bonding effects in the covalent coupling of Penicillin G Acylase

• Published in: Reactive & functional polymers Vol. 64; no. 1; pp. 13 - 24
• Main Authors: Drtina, Gary J., Haddad, Louis C., Rasmussen, Jerald K., Gaddam, Babu N., Williams, Michael G., Moeller, Stephanie J., Fitzsimons, Robert T., Fansler, Duane D., Buhl, Tara L., Yang, Yeng N., Weller, Vallerie A., Lee, Julia M., Beauchamp, Thomas J., Heilmann, Steven M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.2005; Elsevier Science
• Subjects: Applied sciences; Azlactone; Biocatalysis; Biological and medical sciences; Biotechnology; E.C. 3.5.1.11; Emphaze™ AB1; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Immobilization of enzymes and other molecules; Immobilization techniques; Immobilized enzyme; Methods. Procedures. Technologies; Organic polymers; Penicillin G Acylase; Physicochemistry of polymers; Properties and characterization; Special properties (catalyst, reagent or carrier); Biocatalysis; Emphaze™ AB1; Immobilized enzyme; Penicillin G Acylase; Azlactone; E.C. 3.5.1.11; Vinyl derivative copolymer; Methacrylamide copolymer; Support; Enzyme; Immobilization; Oxazole derivative polymer; Hydroxyethyl methacrylate copolymer; Experimental study; Crosslinked copolymer; Penicillin amidase; Enzymatic activity; Polyvinyl derivative; Crosslinked polymer; Emphaze# AB1; Covalent bond; Oxazole derivative copolymer; Hydrolases; Methacrylate copolymer; Comparative study
• ISSN: 1381-5148
• DOI: 10.1016/j.reactfunctpolym.2005.04.004

Azlactone-functional dispersion and reverse phase suspension polymer supports were examined as immobilizing media for Penicillin G Acylase (PGA). Results indicated that the most effective supports were those that also contained primary and/or secondary amide functional groups. A combination of hydrophobic interactions and hydrogen bonding between amide groups on the support and enzyme was proposed to provide important and intimate association between PGA and support prior to covalent coupling. Relative to an oxirane-functional commercial standard, the azlactone biocatalyst supports featured shorter coupling times and higher catalytic activities.