Applications of bottom-up human artificial chromosomes in cell research and cell engineering

• Published in: Experimental cell research Vol. 390; no. 1; p. 111793
• Main Authors: Ikeno, Masashi, Hasegawa, Yoshinori
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2020
• Subjects: Cell Engineering - methods; Chromosomes, Artificial, Human - genetics; Genetic Engineering - methods; Humans; tetR and LacI; hTERT; LINE1; HLA

Chromosome manipulation is a useful technique in biological science. We have constructed human artificial chromosomes (HACs) based on the transfection of centromeric alphoid DNA precursors into cultured human cells. Moreover, HAC-based technology has been developed into a novel gene expression vecto...