Applications of bottom-up human artificial chromosomes in cell research and cell engineering
Chromosome manipulation is a useful technique in biological science. We have constructed human artificial chromosomes (HACs) based on the transfection of centromeric alphoid DNA precursors into cultured human cells. Moreover, HAC-based technology has been developed into a novel gene expression vecto...
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Published in | Experimental cell research Vol. 390; no. 1; p. 111793 |
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Main Authors | , |
Format | Journal Article |
Language | English |
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01.05.2020
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Abstract | Chromosome manipulation is a useful technique in biological science. We have constructed human artificial chromosomes (HACs) based on the transfection of centromeric alphoid DNA precursors into cultured human cells. Moreover, HAC-based technology has been developed into a novel gene expression vector tool for introducing large-size genomic DNA. This technique provides natural expression, as well as stable expression without the gene silencing that often occurs with conventional vectors in mammalian cells. Here we review the properties of HACs, and issues regarding the use of HAC technology for basic and applied research. |
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AbstractList | Chromosome manipulation is a useful technique in biological science. We have constructed human artificial chromosomes (HACs) based on the transfection of centromeric alphoid DNA precursors into cultured human cells. Moreover, HAC-based technology has been developed into a novel gene expression vector tool for introducing large-size genomic DNA. This technique provides natural expression, as well as stable expression without the gene silencing that often occurs with conventional vectors in mammalian cells. Here we review the properties of HACs, and issues regarding the use of HAC technology for basic and applied research. |
ArticleNumber | 111793 |
Author | Ikeno, Masashi Hasegawa, Yoshinori |
Author_xml | – sequence: 1 givenname: Masashi surname: Ikeno fullname: Ikeno, Masashi email: mikeno@aichi-med-u.ac.jp organization: Department of Medical Biology, Aichi Medical University, Nagakute, Aichi, Japan – sequence: 2 givenname: Yoshinori surname: Hasegawa fullname: Hasegawa, Yoshinori organization: Laboratory of Clinical Omics Research, Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31874174$$D View this record in MEDLINE/PubMed |
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CitedBy_id | crossref_primary_10_32607_actanaturae_11670 crossref_primary_10_1007_s00018_020_03653_1 crossref_primary_10_3390_genes13101872 crossref_primary_10_1016_j_yexcr_2020_112302 crossref_primary_10_1016_j_molcel_2022_02_032 crossref_primary_10_1002_cpz1_236 crossref_primary_10_1016_j_isci_2020_101764 crossref_primary_10_1021_acssynbio_2c00580 crossref_primary_10_1002_cpz1_316 crossref_primary_10_1371_journal_pone_0302451 |
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Snippet | Chromosome manipulation is a useful technique in biological science. We have constructed human artificial chromosomes (HACs) based on the transfection of... |
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SubjectTerms | Cell Engineering - methods Chromosomes, Artificial, Human - genetics Genetic Engineering - methods Humans |
Title | Applications of bottom-up human artificial chromosomes in cell research and cell engineering |
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