Applications of bottom-up human artificial chromosomes in cell research and cell engineering

Chromosome manipulation is a useful technique in biological science. We have constructed human artificial chromosomes (HACs) based on the transfection of centromeric alphoid DNA precursors into cultured human cells. Moreover, HAC-based technology has been developed into a novel gene expression vecto...

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Bibliographic Details
Published inExperimental cell research Vol. 390; no. 1; p. 111793
Main Authors Ikeno, Masashi, Hasegawa, Yoshinori
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.05.2020
Subjects
Cell Engineering - methods
Chromosomes, Artificial, Human - genetics
Genetic Engineering - methods
Humans
tetR and LacI
hTERT
LINE1
HLA
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Summary:Chromosome manipulation is a useful technique in biological science. We have constructed human artificial chromosomes (HACs) based on the transfection of centromeric alphoid DNA precursors into cultured human cells. Moreover, HAC-based technology has been developed into a novel gene expression vector tool for introducing large-size genomic DNA. This technique provides natural expression, as well as stable expression without the gene silencing that often occurs with conventional vectors in mammalian cells. Here we review the properties of HACs, and issues regarding the use of HAC technology for basic and applied research.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2019.111793