Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells
Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (...
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Published in | BioTechniques Vol. 22; no. 1; pp. 100 - 105 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Natick, MA
Future Science Ltd
01.01.1997
Eaton |
Subjects | |
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Abstract | Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood. The expression of cytokeratin 19 (K19) was used as the marker by which the isolated tumor cells were identified. The immunobead RT-PCR technique allowed detection of one tumor cell per 10
leukocytes in whole blood. Immunobead RT-PCR is a highly sensitive method of detecting cancer cells in a hematopoietic environment. |
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AbstractList | Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood. The expression of cytokeratin 19 (K19) was used as the marker by which the isolated tumor cells were identified. The immunobead RT-PCR technique allowed detection of one tumor cell per 10(6) leukocytes in whole blood. Immunobead RT-PCR is a highly sensitive method of detecting cancer cells in a hematopoietic environment. Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood. The expression of cytokeratin 19 (K19) was used as the marker by which the isolated tumor cells were identified. The immunobead RT-PCR technique allowed detection of one tumor cell per 106 leukocytes in whole blood. Immunobead RT-PCR is a highly sensitive method of detecting cancer cells in a hematopoietic environment. Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood. The expression of cytokeratin 19 (K19) was used as the marker by which the isolated tumor cells were identified. The immunobead RT-PCR technique allowed detection of one tumor cell per 10 leukocytes in whole blood. Immunobead RT-PCR is a highly sensitive method of detecting cancer cells in a hematopoietic environment. |
Author | Dobrovic, Alexander Eaton, Michael C Kotasek, Dusan Hardingham, Jennifer E |
AuthorAffiliation | 1University of Adelaide, The Queen Elizabeth Hospital, Woodville, SA, Australia |
AuthorAffiliation_xml | – name: 1University of Adelaide, The Queen Elizabeth Hospital, Woodville, SA, Australia |
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Keywords | Human Cytokeratin Immunomagnetic method RNA-directed DNA polymerase Genetic marker Enzyme Transferases Metastasis Epithelium Polymerase chain reaction Mammary gland diseases Nucleotidyltransferases Cell line Detection Tumor cell |
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SubjectTerms | Antibodies, Monoclonal - analysis Biological and medical sciences Biomarkers, Tumor Biotechnology Breast Neoplasms - blood Breast Neoplasms - immunology Carcinoma - blood Carcinoma - immunology Flow Cytometry Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Humans Immunoassay - methods Immunological technics applied to diagnosis Industrial applications and implications. Economical aspects Neoplasm Metastasis Neoplasms - blood Polymerase Chain Reaction - methods Sensitivity and Specificity Tumor Cells, Cultured |
Title | Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells |
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