pH-dependence for binding a single nitrite ion to each type-2 copper centre in the copper-containing nitrite reductase of Alcaligenes xylosoxidans

• Published in: Biochemical journal Vol. 324; no. 2; pp. 511 - 516
• Main Authors: Abraham, Z.H.L, Smith, B.E, Howes, B.D, Lowe, D.J, Eady, R.R
• Format: Journal Article
• Language: English
• Published: England 01.06.1997
• Subjects: Alcaligenes - enzymology; Alcaligenes xylosoxidans; Bacterial Proteins - metabolism; Copper - metabolism; Electron Spin Resonance Spectroscopy; Hydrogen-Ion Concentration; Nitrite Reductases - metabolism; Oxidation-Reduction; plant biochemistry; plant physiology; Protein Binding

The first quantitative characterization of the interaction of NO2(-) with the Cu-containing dissimilatory nitrite reductase (NiR) of Alcaligenes xylosoxidans using steady-state kinetics, equilibrium gel filtration and EPR spectroscopy is described. Each molecule of this protein consists of three equ...