Immunological Characterization of Novel Secreted Antigens of Mycobacterium tuberculosis

Proteins secreted by Mycobacterium tuberculosis are targets of host immune responses and as such are investigated for vaccine and immunodiagnostics development. Computer‐driven searches of the M. tuberculosis H37Rv genome had previously identified 45 novel secreted proteins. Here, we report the char...

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Published inScandinavian journal of immunology Vol. 61; no. 2; pp. 139 - 146
Main Authors Amor, Y. B., Shashkina, E., Johnson, S., Bifani, P. J., Kurepina, N., Kreiswirth, B., Bhattacharya, S., Spencer, J., Rendon, A., Catanzaro, A., Gennaro, M. L.
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LanguageEnglish
Published Oxford, UK; Malden, USA Blackwell Science Ltd 01.02.2005
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Abstract Proteins secreted by Mycobacterium tuberculosis are targets of host immune responses and as such are investigated for vaccine and immunodiagnostics development. Computer‐driven searches of the M. tuberculosis H37Rv genome had previously identified 45 novel secreted proteins. Here, we report the characterization of these antigens in terms of specificity for the M. tuberculosis complex and the ability to induce human immune responses. BLAST homology searches and Southern hybridization identified 10 genes that were either specific for the M. tuberculosis complex or found in only two nontuberculous mycobacterial species of minor medical significance. Selected recombinant proteins were purified from Escherichia coli cells and tested for the ability to elicit antibody responses in tuberculosis patients. Reactivity of the serum panel was ` 36% with at least one of five novel proteins (Rv0203, Rv0603, Rv1271c, Rv1804c and Rv2253), 56% with the 38 kDa lipoprotein, a M. tuberculosis antigen known to be highly seroreactive, and 68% with a combination of Rv0203, Rv1271c and the 38 kDa antigen. Thus, at least five novel secreted proteins induce antibody responses during active disease; some of these proteins may increase the sensitivity of serological assays based on the 38 kDa antigen.
AbstractList Proteins secreted by Mycobacterium tuberculosis are targets of host immune responses and as such are investigated for vaccine and immunodiagnostics development. Computer‐driven searches of the M. tuberculosis H37Rv genome had previously identified 45 novel secreted proteins. Here, we report the characterization of these antigens in terms of specificity for the M. tuberculosis complex and the ability to induce human immune responses. BLAST homology searches and Southern hybridization identified 10 genes that were either specific for the M. tuberculosis complex or found in only two nontuberculous mycobacterial species of minor medical significance. Selected recombinant proteins were purified from Escherichia coli cells and tested for the ability to elicit antibody responses in tuberculosis patients. Reactivity of the serum panel was ` 36% with at least one of five novel proteins (Rv0203, Rv0603, Rv1271c, Rv1804c and Rv2253), 56% with the 38 kDa lipoprotein, a M. tuberculosis antigen known to be highly seroreactive, and 68% with a combination of Rv0203, Rv1271c and the 38 kDa antigen. Thus, at least five novel secreted proteins induce antibody responses during active disease; some of these proteins may increase the sensitivity of serological assays based on the 38 kDa antigen.
Proteins secreted by Mycobacterium tuberculosis are targets of host immune responses and as such are investigated for vaccine and immunodiagnostics development. Computer-driven searches of the M. tuberculosis H sub(37)Rv genome had previously identified 45 novel secreted proteins. Here, we report the characterization of these antigens in terms of specificity for the M. tuberculosis complex and the ability to induce human immune responses. BLAST homology searches and Southern hybridization identified 10 genes that were either specific for the M. tuberculosis complex or found in only two nontuberculous mycobacterial species of minor medical significance. Selected recombinant proteins were purified from Escherichia coli cells and tested for the ability to elicit antibody responses in tuberculosis patients. Reactivity of the serum panel was ` 36% with at least one of five novel proteins (Rv0203, Rv0603, Rv1271c, Rv1804c and Rv2253), 56% with the 38 kDa lipoprotein, a M. tuberculosis antigen known to be highly seroreactive, and 68% with a combination of Rv0203, Rv1271c and the 38 kDa antigen. Thus, at least five novel secreted proteins induce antibody responses during active disease; some of these proteins may increase the sensitivity of serological assays based on the 38 kDa antigen.
Abstract Proteins secreted by Mycobacterium tuberculosis are targets of host immune responses and as such are investigated for vaccine and immunodiagnostics development. Computer‐driven searches of the M. tuberculosis H 37 Rv genome had previously identified 45 novel secreted proteins. Here, we report the characterization of these antigens in terms of specificity for the M. tuberculosis complex and the ability to induce human immune responses. BLAST homology searches and Southern hybridization identified 10 genes that were either specific for the M. tuberculosis complex or found in only two nontuberculous mycobacterial species of minor medical significance. Selected recombinant proteins were purified from Escherichia coli cells and tested for the ability to elicit antibody responses in tuberculosis patients. Reactivity of the serum panel was ` 36% with at least one of five novel proteins (Rv0203, Rv0603, Rv1271c, Rv1804c and Rv2253), 56% with the 38 kDa lipoprotein, a M. tuberculosis antigen known to be highly seroreactive, and 68% with a combination of Rv0203, Rv1271c and the 38 kDa antigen. Thus, at least five novel secreted proteins induce antibody responses during active disease; some of these proteins may increase the sensitivity of serological assays based on the 38 kDa antigen.
Author Gennaro, M. L.
Bifani, P. J.
Catanzaro, A.
Amor, Y. B.
Spencer, J.
Kreiswirth, B.
Shashkina, E.
Kurepina, N.
Rendon, A.
Bhattacharya, S.
Johnson, S.
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Snippet Proteins secreted by Mycobacterium tuberculosis are targets of host immune responses and as such are investigated for vaccine and immunodiagnostics...
Abstract Proteins secreted by Mycobacterium tuberculosis are targets of host immune responses and as such are investigated for vaccine and immunodiagnostics...
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StartPage 139
SubjectTerms Antibodies, Bacterial - blood
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Blotting, Western
Cloning, Molecular
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Enzyme-Linked Immunosorbent Assay
Humans
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - immunology
Polymerase Chain Reaction
Recombinant Proteins - immunology
Tuberculosis, Pulmonary - immunology
Tuberculosis, Pulmonary - microbiology
Title Immunological Characterization of Novel Secreted Antigens of Mycobacterium tuberculosis
URI https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.0300-9475.2005.01557.x
https://www.ncbi.nlm.nih.gov/pubmed/15683450
https://www.proquest.com/docview/198124401
https://search.proquest.com/docview/17623927
https://search.proquest.com/docview/67401236
Volume 61
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