Low glucose concentrations within typical industrial operating conditions have minimal effect on the transcriptome of recombinant CHO cells

• Published in: Biotechnology progress Vol. 33; no. 3; pp. 771 - 785
• Main Authors: Gowtham, Yogender Kumar, Saski, Christopher A., Harcum, Sarah W.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.05.2017
• Subjects: Animals; Batch Cell Culture Techniques; CHO Cells; Cricetinae; Cricetulus; Gene expression; Genes; Glucose; Glucose - pharmacology; Glycolysis; Glycolysis - drug effects; Glycolysis - genetics; glycosylation; Glycosylation - drug effects; Mammals; Monoclonal antibodies; Ribonucleic acid; RNA; RNA‐Seq; Transcriptome - drug effects; Transcriptome - genetics; Glycolysis; glycosylation; RNA-Seq; gene expression

Typically, mammalian cell culture medium contains high glucose concentrations that are analogous to diabetic levels in humans, suggesting that mammalian cells are cultivated in excessive glucose. Using RNA‐Seq, this study characterized the Chinese hamster ovary (CHO) cell transcriptome under two glu...