Development of a Novel CD4 + TCR Transgenic Line That Reveals a Dominant Role for CD8 + Dendritic Cells and CD40 Signaling in the Generation of Helper and CTL Responses to Blood-Stage Malaria

We describe an MHC class II (I-A )-restricted TCR transgenic mouse line that produces CD4 T cells specific for species. This line, termed PbT-II, was derived from a CD4 T cell hybridoma generated to blood-stage ANKA (PbA). PbT-II cells responded to all species and stages tested so far, including rod...

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Published inThe Journal of immunology (1950) Vol. 199; no. 12; pp. 4165 - 4179
Main Authors Fernandez-Ruiz, Daniel, Lau, Lei Shong, Ghazanfari, Nazanin, Jones, Claerwen M, Ng, Wei Yi, Davey, Gayle M, Berthold, Dorothee, Holz, Lauren, Kato, Yu, Enders, Matthias H, Bayarsaikhan, Ganchimeg, Hendriks, Sanne H, Lansink, Lianne I M, Engel, Jessica A, Soon, Megan S F, James, Kylie R, Cozijnsen, Anton, Mollard, Vanessa, Uboldi, Alessandro D, Tonkin, Christopher J, de Koning-Ward, Tania F, Gilson, Paul R, Kaisho, Tsuneyasu, Haque, Ashraful, Crabb, Brendan S, Carbone, Francis R, McFadden, Geoffrey I, Heath, William R
Format Journal Article
LanguageEnglish
Published United States American Association of Immunologists 15.12.2017
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Summary:We describe an MHC class II (I-A )-restricted TCR transgenic mouse line that produces CD4 T cells specific for species. This line, termed PbT-II, was derived from a CD4 T cell hybridoma generated to blood-stage ANKA (PbA). PbT-II cells responded to all species and stages tested so far, including rodent (PbA, NK65, AS, and 17XNL) and human ( ) blood-stage parasites as well as irradiated PbA sporozoites. PbT-II cells can provide help for generation of Ab to infection and can control this otherwise lethal infection in CD40L-deficient mice. PbT-II cells can also provide help for development of CD8 T cell-mediated experimental cerebral malaria (ECM) during PbA infection. Using PbT-II CD4 T cells and the previously described PbT-I CD8 T cells, we determined the dendritic cell (DC) subsets responsible for immunity to PbA blood-stage infection. CD8 DC (a subset of XCR1 DC) were the major APC responsible for activation of both T cell subsets, although other DC also contributed to CD4 T cell responses. Depletion of CD8 DC at the beginning of infection prevented ECM development and impaired both Th1 and follicular Th cell responses; in contrast, late depletion did not affect ECM. This study describes a novel and versatile tool for examining CD4 T cell immunity during malaria and provides evidence that CD4 T cell help, acting via CD40L signaling, can promote immunity or pathology to blood-stage malaria largely through Ag presentation by CD8 DC.
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D.F.-R. designed and performed the research and wrote the manuscript; L.S.L., N.G., C.M.J., W.Y.N., G.M.D., D.B., L.H., Y.K., G.B., S.H.H., K.R.J., M.H.E., L.I.M.L., J.A.E., and M.S.F.S. performed research; A.C., V.M., T.F.d.K.-W., P.R.G., A.D.U., C.J.T., T.K., and G.I.M. provided reagents; A.H., B.S.C., and F.R.C. designed the research; W.R.H. designed the research and wrote the manuscript.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.1700186