Dose-response in a high density three-dimensional liver device with real-time bioenergetic and metabolic flux quantification

• Published in: Toxicology in vitro Vol. 45; no. Pt 1; pp. 119 - 127
• Main Authors: Tikunov, Andrey P., Shim, Yoo-Sik, Bhattarai, Narayan, Siler, Scott Q., Soldatow, Valerie, LeCluyse, Edward L., McDunn, Jonathan E., Watkins, Paul B., Macdonald, Jeffrey M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2017; Elsevier Science Ltd
• Subjects: 31P and 13C NMR spectroscopy; Alginate-hepatocyte encapsulation; Alginates; Alginic acid; Animal tissues; Animals; Artificial Organs; ATP; Bioenergetics; Bioreactors; Biosynthesis; Carbon 13; Cell culture; Computational Biology; Computer applications; Density; Dosage; Dose response; Drug safety; Fluidized bed reactors; Fluidized beds; Fluidized-bed bioreactor; Fructose; Glucose; Glutathione; Glycine; Glycogen; Glycolysis; Hepatocytes; Hepatocytes - physiology; Lactic acid; Liver; Liver - physiology; Magnetic resonance spectroscopy; Mammalian cells; Mass transfer; Membrane reactors; Metabolic flux; Metabolites; NMR spectroscopy; NMR-compatible; Nutrient concentrations; Nutrient loading; Oxygen; Perfusion; Phosphates; Rats; Rats, Wistar; Real time; Spectroscopy; Spheroids; Fluidized-bed bioreactor; NMR-compatible; Alginate-hepatocyte encapsulation; Dose response; Drug safety; 31P and 13C NMR spectroscopy; Fructose; P and C NMR spectroscopy

Real-time dose-response curves for fructose have been non-invasively determined in primary rat hepatocyte alginate spheroids cultured in a NMR-compatible fluidized-bed bioreactor. Using 13C–labeled glucose and glycine culture medium, fructose dose was compared to glucose uptake and glycogen synthesi...