Development and evaluation of a ligation-free sequence-independent, single-primer amplification (LF-SISPA) assay for whole genome characterization of viruses

• Published in: Journal of virological methods Vol. 299; p. 114346
• Main Authors: Jothikumar, Narayanan, Cromeans, Theresa, Shivajothi, Jothikumar, Vinjé, Jan, Murphy, Jennifer
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2022
• Subjects: Genome, Viral; High-Throughput Nucleotide Sequencing - methods; Humans; Metagenomics; Norovirus; Polymerase Chain Reaction; RNA and DNA viruses; Sequence Analysis, DNA - methods; SISPA; Viruses - genetics; Whole genome sequencing; Metagenomics; SISPA; Norovirus; RNA and DNA viruses; Whole genome sequencing

•LF-SISPA NGS method requires no commercial library preparation kits.•Save time, cost, and labor in a simplified library preparation.•LF-SISPA NGS identifies a wide range of RNA and DNA viruses.•LF-SISPA allows resource limited laboratories to prepare their sequencing libraries. Molecular identifica...