Preparation of Chicken Anemia Virus (CAV) Virus-Like Particles and Chicken Interleukin-12 for Vaccine Development Using a Baculovirus Expression System

• Published in: Pathogens (Basel) Vol. 8; no. 4; p. 262
• Main Authors: Tseng, Ta-Yuan, Liu, Yee-Chen, Hsu, Yu-Chen, Chang, Poa-Chun, Hsieh, Ming-Kun, Shien, Jui-Hung, Ou, Shan-Chia
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.11.2019; MDPI
• Subjects: Anemia; Animal diseases; Antibodies; Baculovirus; Biological activity; Cell-mediated immunity; Chickens; Cytokines; E coli; Economic impact; Embryos; Immunofluorescence; Infectious anemia; Interferon; Interleukin 12; Monoclonal antibodies; Pathogens; Polyclonal antibodies; Poultry; Proteins; Self-assembly; Signs and symptoms; Specific pathogen free; Splenocytes; Structural proteins; Transmission electron microscopy; Vaccine development; Vaccines; Virus-like particles; Viruses; VP1 protein; Western blotting; γ-Interferon; chicken anemia virus; vaccine development; chicken IL-12; adjuvant; virus-like particle; chicken infectious anemia vaccine

Chicken infectious anemia (CIA) is a poultry disease that causes huge economic losses in the poultry industry worldwide. Commercially available CIA vaccines are derived from wild-type chicken anemia viruses (CAVs) by serial passage in cells or chicken embryos. However, these vaccinal viruses are not...