Immune-Enhancing Effects of Lactobacillus plantarum 200655 Isolated from Korean Kimchi in a Cyclophosphamide-Induced Immunocompromised Mouse Model

• Published in: Journal of microbiology and biotechnology Vol. 31; no. 5; pp. 726 - 732
• Main Authors: Kim, Kyeong Jin, Paik, Hyun-Dong, Kim, Ji Yeon
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Microbiology and Biotechnology 28.05.2021; 한국미생물·생명공학회
• Subjects: Alanine Transaminase - blood; Animals; Cyclophosphamide - pharmacology; Cytokines - metabolism; Disease Models, Animal; Fermented Foods - microbiology; Immunocompromised Host - drug effects; Immunocompromised Host - immunology; Killer Cells, Natural - drug effects; Killer Cells, Natural - immunology; Lactobacillus plantarum - isolation & purification; MAP Kinase Signaling System - drug effects; Mice; Mice, Inbred ICR; Probiotics - administration & dosage; Probiotics - pharmacology; Republic of Korea; Research article; Spleen - drug effects; Spleen - immunology; 생물학; Republic of Korea; immune enhancement; Cyclophosphamide; Lactobacillus plantarum; splenocytes; MAPK/ERK signaling pathway
• DOI: 10.4014/jmb.2103.03028
• ISSN: 1017-7825

In this study, we evaluated the immune-enhancing activity of kimchi-derived 200655 on immune suppression by cyclophosphamide (CP) in ICR mice. Animals were fed distilled water or 1×10 colony-forming unit/kg B.W. 200655 or GG as a positive control for 14 days. An in vivo model of immunosuppression was induced using CP 150 and 100 mg/kg B.W. at 7 and 10 days, respectively. Body weight, spleen index, spleen weight, and gene expression were measured to estimate the immune-enhancing effects. The dead 200655 (D-200655) group showed an increased spleen weight compared to the sham control (SC) group. Similarly, the spleen index was significantly higher than that in the CP-treated group. The live 200655 (L-200655) group showed an increased mRNA expression of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 in splenocytes. Also, the iNOS and COX-2 mRNA expression was upregulated in the L-200655 group compared to the CP-only (SC) group. The phosphorylation of ERK and MAPK was also upmodulated in the L-200655 group. These results indicate that 200655 ameliorated CP-induced immune suppression, suggesting that 200655 may have the potential to enhance the immune system.