Up-regulation of neutrophil gelatinase-associated lipocalin in oral squamous cell carcinoma: Relation to cell differentiation
Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin2, LCN2) is a secreted glycoprotein with increased expression in solid tumors. The expression and functions of NGAL in oral cancer, however, remain unclear. We investigated the expression of NGAL in oral cancer tissues and oral...
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Published in | Oncology reports Vol. 26; no. 6; pp. 1415 - 1421 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
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Spandidos
01.12.2011
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Abstract | Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin2, LCN2) is a secreted glycoprotein with increased expression in solid tumors. The expression and functions of NGAL in oral cancer, however, remain unclear. We investigated the expression of NGAL in oral cancer tissues and oral cancer cell lines. By immunohistochemical examinations, NGAL expression was strongly up-regulated in well-differentiated OSCC tissues and moderately to weakly up-regulated in moderately to poorly differentiated OSCC tissues. In contrast, NGAL expression was weak or very weak in normal mucosa and leukoplakia. By western blot analysis, NGAL expression levels positively correlated with cell morphology patterns and loss of E-cadherin. In addition, the enzymatic activity of the NGAL/MMP-9 complex significantly correlated with the results obtained by zymographic analysis. In conclusion, NGAL expression is high in well-differentiated cancer, suggesting that NGAL may be a useful diagnostic marker of tumor-cell differentiation. |
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AbstractList | Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin2, LCN2) is a secreted glycoprotein with increased expression in solid tumors. The expression and functions of NGAL in oral cancer, however, remain unclear. We investigated the expression of NGAL in oral cancer tissues and oral cancer cell lines. By immunohistochemical examinations, NGAL expression was strongly up-regulated in well-differentiated OSCC tissues and moderately to weakly up-regulated in moderately to poorly differentiated OSCC tissues. In contrast, NGAL expression was weak or very weak in normal mucosa and leukoplakia. By western blot analysis, NGAL expression levels positively correlated with cell morphology patterns and loss of E-cadherin. In addition, the enzymatic activity of the NGAL/MMP-9 complex significantly correlated with the results obtained by zymographic analysis. In conclusion, NGAL expression is high in well-differentiated cancer, suggesting that NGAL may be a useful diagnostic marker of tumor-cell differentiation. |
Author | HIROMOTO, Takashi MORIDERA, Kuniyasu KISHIMOTO, Hiromitsu NOGUCHI, Kazuma SEGAWA, Emi ZUSHI, Yusuke YAMAMURA, Michiyo TAKAOKA, Kazuki URADE, Masahiro |
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Keywords | differentiation Oral cancer Stomatology neutrophil gelatinase-associated lipocalin lipocalin2 Malignant tumor Oral squamous cell carcinoma Cell differentiation Cancerology Metastasis suppressor gene Oral cavity disease Neutrophil gelatinase associated lipocalin Cancer |
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SubjectTerms | Acute-Phase Proteins - genetics Acute-Phase Proteins - metabolism Adult Aged Aged, 80 and over Biological and medical sciences Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Cadherins - metabolism Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Cell Differentiation Cell Line, Tumor Enzyme Assays Female Humans Lipocalin-2 Lipocalins - genetics Lipocalins - metabolism Male Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 - metabolism Medical sciences Middle Aged Neoplasm Grading Otorhinolaryngology. Stomatology Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Receptor, Epidermal Growth Factor - metabolism Tongue Neoplasms - metabolism Tongue Neoplasms - pathology Tumors Up-Regulation Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
Title | Up-regulation of neutrophil gelatinase-associated lipocalin in oral squamous cell carcinoma: Relation to cell differentiation |
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