Characterization of the effects of C-terminal pro-sequence on self-inactivation of Stereum purpureum endopolygalacturonase I

• Published in: FEMS microbiology letters Vol. 362; no. 17; p. fnv134
• Main Authors: Hamada, Shigeki, Toda, Kensuke, Ogawa, Sayaka, Kubota, Keisuke, Miyairi, Kazuo
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.09.2015
• Subjects: Agaricales - enzymology; Agaricales - genetics; Alanine; Amino Acid Sequence; Deactivation; Endopolygalacturonase; Enzyme Precursors - chemistry; Enzyme Precursors - metabolism; Glutamates; Hydrogen-Ion Concentration; Inactivation; Kinetics; Malus - microbiology; Microbiology; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Pectins - metabolism; pH effects; Plant diseases; Polygalacturonase - chemistry; Polygalacturonase - genetics; Polygalacturonase - metabolism; Sequence Homology, Amino Acid; Serine Endopeptidases - metabolism; Silver-leaf disease; Stereum purpureum; Substrate preferences; Substrates; site-direct mutagenesis; endopolygalacturonase; kinetics; pro-sequence; inactivation; pectin; Stereum purpureum

Endpolygalacturonase I from Stereum purpureum has been identified as a causative substance for the silver-leaf disease in apples. It possesses a unique pro-sequence in the C-terminal region that lacks endpolygalacturonases from any other origin. In this study, we analyzed and compared enzymatic char...