Functional co-expression of xenobiotic metabolizing enzymes, rat cytochrome P450 1A1 and UDP-glucuronosyltransferase 1A6, in yeast microsomes

• Published in: Biochimica et biophysica acta Vol. 1672; no. 2; pp. 86 - 92
• Main Authors: Ikushiro, Shin-ichi, Sahara, Masahiro, Emi, Yoshikazu, Yabusaki, Yoshiyasu, Iyanagi, Takashi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 03.05.2004
• Subjects: 7-ethoxycoumarin; Animals; Blotting, Western; Co-expression system; Cytochrome P-450 CYP1A1 - metabolism; Cytochrome P450; Electrophoresis, Polyacrylamide Gel; Glucuronosyltransferase - metabolism; Male; Microsomes - enzymology; Rats; Rats, Wistar; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; UDP-glucuronosyltransferase; Xenobiotic; Xenobiotics - metabolism; Yeast microsome; AbA; Yeast microsome; UGT; Co-expression system; UDP-glucuronosyltransferase; Cytochrome P450; ER; P450 reductase; GlcUA; mEH; 7HC; 7GC; 7EC; 7-ethoxycoumarin; 4-MU; P450; GAPDH; Xenobiotic; HPLC

Xenobiotic Phase I and Phase II reactions in hepatocytes occur sequentially and cooperatively during the metabolism of various chemical compounds including drugs. In order to investigate the sequential metabolism of 7-ethoxycoumarin (7EC) as model substrate in vitro, xenobiotic metabolizing enzymes,...