Passive immunization of immune-suppressed animals: Chicken antibodies protect against Ornithobacterium rhinotracheale infection

• Published in: Vaccine Vol. 23; no. 26; pp. 3404 - 3411
• Main Authors: Schuijffel, D.F., Empel, P.C.M. van, Pennings, A.M.M.A., Putten, J.P.M. van, Nuijten, P.J.M.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 16.05.2005; Elsevier
• Subjects: (Cross-) protection; Animals; Antibodies, Bacterial - administration & dosage; Antibodies, Bacterial - immunology; Applied microbiology; B cell depletion; Biological and medical sciences; Chickens; Flavobacteriaceae Infections - prevention & control; Flavobacteriaceae Infections - veterinary; Fundamental and applied biological sciences. Psychology; Immunity, Cellular; Immunization; Immunization, Passive; Microbiology; Ornithobacterium; Ornithobacterium rhinotracheale; Passive serum transfer; Vaccine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Ornithobacterium rhinotracheale; Vaccine; B cell depletion; Passive serum transfer; (Cross-) protection; Vaccine: B cell depletion; Farming animal; Poultry; Antibody; Cross protection; B-Lymphocyte; Vertebrata; Serum; Chicken; Aves; Veterinary; Passive immunization
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2005.01.095

Unravelling of the protective immunity acquired during a natural infection may contribute to vaccine development. To assess the role of antibody-mediated immunity in protection against Ornithobacterium rhinotracheale infection in chickens, a novel experimental method was applied that combined immune depletion and passive transfer of immunity within the same host. Administration of cyclophosphamide (CY) to broiler chickens successfully suppressed B lymphocyte development, and therefore humoral immunity, as confirmed by histological and serological analysis. Challenge of CY-treated birds with O. rhinotracheale revealed a significantly higher pathology score in comparison to immune-competent birds that received the same bacterial challenge. Measurement of serum immunoglobulin levels of immune-competent birds revealed a positive correlation between IgA and/or IgG production and protection against infection. Passive transfer of O. rhinotracheale-specific antiserum to the immune-suppressed birds prior to pathogen challenge significantly decreased morbidity. This protective effect was not observed after administration of control sera containing similar concentrations of immunoglobulins. Together, these results provide firm evidence that chicken humoral immunity to O. rhinotracheale is a key component in protection against infection. Our data confirm that the applied immune depletion and reconstitution approach is an attractive tool to analyse the nature of the protective immune response.