Effects of pH Shift and Agitation Speed on Chlorophyll Biosynthesis in Heterotrophic Culture of Chlorella
Chlorella sp., which has been used for industrial biomass production, was cultured under a heterotrophic condition. When pH was kept at 6.0 during the initial period of the culture, corresponding to logarithmic growth of cell, and thereafter shifted to 8.0, the instantaneous specific production rate...
Saved in:
| Published in | KAGAKU KOGAKU RONBUNSHU Vol. 25; no. 2; pp. 214 - 219 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
The Society of Chemical Engineers, Japan
1999
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0386-216X 1349-9203 |
| DOI | 10.1252/kakoronbunshu.25.214 |
Cover
| Abstract | Chlorella sp., which has been used for industrial biomass production, was cultured under a heterotrophic condition. When pH was kept at 6.0 during the initial period of the culture, corresponding to logarithmic growth of cell, and thereafter shifted to 8.0, the instantaneous specific production rates of chlorophyll (CHL) throughout the culture were maintained at relatively high values. This pH shift pattern was proposed as the optimum one. The maximum CHL content under the optimum pH shift, however, was only 1.28 W/W % at the agitation speed of 600 r.p.m.. On the other hand, low agitation speeds less than 300 r.p.m. were more favorable for CHL biosynthesis. The maximum CHL content reached 3.1 W/W % at the agitation speed of 250 r.p.m.. This value was about 2.4 times higher than that in the case of 600 r.p.m.. It was suggested that the effect of agitation speed on CHL biosynthesis depended on the existence of a period lacking oxygen supply. Though the maximum CHL content reached also about 3 W/W % under the constant pH value of 6.0 at agitation speed of 250 r.p.m., the specific production rate of CHL during the middle period of the culture was 0.54 × 10 kg/ (kg dry cell·h), and about 70 % of that in the case of the optimum pH shift. This result revealed that CHL biosynthesis in the heterotrophic culture of Chlorella sp. was carried out more efficiently under both the optimum pH shift and the agitation speed of 250 r.p.m.. |
|---|---|
| AbstractList | Chlorella sp., which has been used for industrial biomass production, was cultured under a heterotrophic condition. When pH was kept at 6.0 during the initial period of the culture, corresponding to logarithmic growth of cell, and thereafter shifted to 8.0, the instantaneous specific production rates of chlorophyll (CHL) throughout the culture were maintained at relatively high values. This pH shift pattern was proposed as the optimum one. The maximum CHL content under the optimum pH shift, however, was only 1.28 W/W % at the agitation speed of 600 r.p.m.. On the other hand, low agitation speeds less than 300 r.p.m. were more favorable for CHL biosynthesis. The maximum CHL content reached 3.1 W/W % at the agitation speed of 250 r.p.m.. This value was about 2.4 times higher than that in the case of 600 r.p.m.. It was suggested that the effect of agitation speed on CHL biosynthesis depended on the existence of a period lacking oxygen supply. Though the maximum CHL content reached also about 3 W/W % under the constant pH value of 6.0 at agitation speed of 250 r.p.m., the specific production rate of CHL during the middle period of the culture was 0.54 × 10 kg/ (kg dry cell·h), and about 70 % of that in the case of the optimum pH shift. This result revealed that CHL biosynthesis in the heterotrophic culture of Chlorella sp. was carried out more efficiently under both the optimum pH shift and the agitation speed of 250 r.p.m.. |
| Author | FUNAHASHI, HITOSHI NISHIYAMA, YUKITAKA ASANO, MASAKI NAKAO, KATSUMI |
| Author_xml | – sequence: 1 fullname: ASANO, MASAKI organization: Department of Applied Chemistry and Chemical Engineering, Yamaguchi University – sequence: 1 fullname: NISHIYAMA, YUKITAKA organization: Department of Applied Chemistry and Chemical Engineering, Yamaguchi University – sequence: 1 fullname: FUNAHASHI, HITOSHI organization: Department of Applied Chemistry and Chemical Engineering, Yamaguchi University – sequence: 1 fullname: NAKAO, KATSUMI organization: Department of Applied Chemistry and Chemical Engineering, Yamaguchi University |
| BookMark | eNqFkE1LAzEQhoNUsNb-Aw_5A1uTbLIf3mqpVqh4qIK3JZuddGNjtiTpof_eflGoFy8zAzPPvPDcop7rHCB0T8mIMsEeVnLV-c7VGxfazYiJEaP8CvVpysukZCTtoT5JiyxhNPu6QcMQTE0I4wVPy6KPzFRrUDHgTuP1DC9aoyOWrsHjpYkyms7hxRqgwbth0tpd0rrdWoufTBe2LrYQTMDG4RlE8F3cr43Ck42NGw_7pwcIrJV36FpLG2B46gP0-Tz9mMyS-fvL62Q8TxRnPCZcqEIXuVKCURCN0I2Uec0EkJxz4HlWN5A3SqdE1zLNSq6VzgUUdcmKuiBZOkD8-Ff5LgQPulp78yP9tqKk2hurLoxVTFQ7Yzvs8Q-mTgKil8b-B78d4e8Q5RLOidJHoyxcQrTMxQE8FcrPd6qVvgKX_gIpCJjL |
| CitedBy_id | crossref_primary_10_1080_26388081_2020_1715256 |
| ContentType | Journal Article |
| Copyright | by THE SOCIETY OF CHEMICAL ENGINEERS, JAPAN |
| Copyright_xml | – notice: by THE SOCIETY OF CHEMICAL ENGINEERS, JAPAN |
| DBID | AAYXX CITATION |
| DOI | 10.1252/kakoronbunshu.25.214 |
| DatabaseName | CrossRef |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering |
| EISSN | 1349-9203 |
| EndPage | 219 |
| ExternalDocumentID | 10_1252_kakoronbunshu_25_214 article_kakoronbunshu1975_25_2_25_2_214_article_char_en |
| GroupedDBID | .LE 5GY ACGFO AENEX ALMA_UNASSIGNED_HOLDINGS CS3 EBS EJD HH5 JSF P2P RJT AAYXX CITATION E3Z XSB |
| ID | FETCH-LOGICAL-c424t-45c8f87cc521e5d5fdaa7b25e0744e476bde7dcf30fba3694fcf75e8b928b8063 |
| ISSN | 0386-216X |
| IngestDate | Tue Jul 01 01:58:45 EDT 2025 Thu Apr 24 23:00:22 EDT 2025 Wed Sep 03 06:30:16 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Language | English Japanese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c424t-45c8f87cc521e5d5fdaa7b25e0744e476bde7dcf30fba3694fcf75e8b928b8063 |
| OpenAccessLink | https://www.jstage.jst.go.jp/article/kakoronbunshu1975/25/2/25_2_214/_article/-char/en |
| PageCount | 6 |
| ParticipantIDs | crossref_primary_10_1252_kakoronbunshu_25_214 crossref_citationtrail_10_1252_kakoronbunshu_25_214 jstage_primary_article_kakoronbunshu1975_25_2_25_2_214_article_char_en |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 1900 |
| PublicationDate | 1999-00-00 |
| PublicationDateYYYYMMDD | 1999-01-01 |
| PublicationDate_xml | – year: 1999 text: 1999-00-00 |
| PublicationDecade | 1990 |
| PublicationTitle | KAGAKU KOGAKU RONBUNSHU |
| PublicationTitleAlternate | KAGAKU KOGAKU RONBUNSHU |
| PublicationYear | 1999 |
| Publisher | The Society of Chemical Engineers, Japan |
| Publisher_xml | – name: The Society of Chemical Engineers, Japan |
| References | Hirata, S., M. Taya and S. Tone ; “Effect of Temperature on Change in Cell Diameter of Chlorella sp. UK 001 in Photoautotrophic Culture, ” Kagaku Kogaku Ronbunshu, 23,200-207 (1997) Hirata, S., M. Taya and S.Tone ; “Characterization of Chlorella Cell Cultures in Batch and Continuous Operations under a Photoautotrophic Condition, ” J. Chem. Eng. Japan, 29,953-959 (1996) Huang, L. and P. A. Castelfranco ; “Regulation of 5-Aminolevulinic Acid (ALA) Synthesis in Developing Chloroplasts. III. Evidence for Functional Heterogeneity of the ALA Pool, ” Plant Physiol., 92,172-178 (1990) Ogbonna, J. C., H. Yada, H. Yasui and H. Tanaka ; “A Novel Internally Illuminated Stirred Tank Photobioreactor for Large-Scale Cultivation of Photosynthetic Cells, ” J. Ferment. Bioeng., 82, 61-67 (1996) Endo, H., H. Hosoya and T. Koibuchi ; “Growth Yields of Chlorella regularis in Dark-heterotrophic Continuous Cultures Using Ace-tate, ” J. Ferment. Technol., 55,369-379 (1977) Comar, C. L. and F. P. Zscheile ; “Analysis of Plant Extracts for Chlorophylls a and b by a Photoelectric Spectrophotometric Method, ” Plant Physiol., 17,198-209 (1942) Ogbonna, J. C., H. Yada and H. Tanaka ; “Effect of Cell Movement by Random Mixing Between the Surface and Bottom of Photobioreactor, ” J. Ferment. Bioeng., 79,152-157 (1995 a) Javanmardian, M. and B. O. Palsson ; “High-Density Photoautotrophic Algal Cultures : Design, Construction, and Operation of a Novel Photobioreactor System, ” Biotechnol. Bioeng., 38, 1182-1189 (1991) Bandyopadhyay, B., A. E. Humphrey and H. Taguchi ; “Dynamic Measurement of the Volumetric Oxygen Transfer Coefficient in Fermentation Systems, ” Biotechnol. Bioeng., 9,533-544 (1967) Weinstein, J. D., R. W. Howell, R. D. Leverette, S. Y. Grooms, P. S. Brignola, S. M. Mayer and S. I. Beale ; “Heme Inhibition of δ-Aminolevulinic Acid Synthesis Is Enhanced by Glutathione in Cell-Free Extracts of Chlorella, ” Plant Physiol., 101,657-665 (1993) Tigier, H. A., A. M. del C. Batlle and G. Locascio ; “Porphyrin Biosynthesis in Soybean Callus Tissue System. Isolation, Purification and General Properties of delta-Aminolevulinate Dehydratase, ” Biochim. Biophys. Acta, 15 300-302 (1968) Hirata, S., K. Nagayasu, M. Taya and S.Tone ; “The Effects of Environmental Factors on the Growth of a Microalga Chlorella sp. UK 001, ” Proc. of 28 th Autumn Meeting of the SCEJ., vol. 1, p. 334, Sapporo, Japan (1995) Tanaka, A., R. Tanaka, H. Ito, K. Yoshida ; “Kurorofiru No Seigousei, ” Tanpakushitsu Kakusan Kouso, 42,136-145 (1996) Endo, H., K. Nakajima, R. Chino and M. Shirota ; “Growth Characteristic and Cellular Components of Chlorella regularis, Heterotrophic Fast Growing Strain, ” Agr. Biol. Chem., 38, 9-18 (1974) Ogbonna, J. C., H. Yada and H. Tanaka ; “Light Supply Coefficient : a New Engineering Parameter for Photobioreactor Design, ” J. Ferment. Bioeng., 80,369-376 (1995 b) |
| References_xml | – reference: Hirata, S., M. Taya and S.Tone ; “Characterization of Chlorella Cell Cultures in Batch and Continuous Operations under a Photoautotrophic Condition, ” J. Chem. Eng. Japan, 29,953-959 (1996) – reference: Endo, H., K. Nakajima, R. Chino and M. Shirota ; “Growth Characteristic and Cellular Components of Chlorella regularis, Heterotrophic Fast Growing Strain, ” Agr. Biol. Chem., 38, 9-18 (1974) – reference: Javanmardian, M. and B. O. Palsson ; “High-Density Photoautotrophic Algal Cultures : Design, Construction, and Operation of a Novel Photobioreactor System, ” Biotechnol. Bioeng., 38, 1182-1189 (1991) – reference: Hirata, S., K. Nagayasu, M. Taya and S.Tone ; “The Effects of Environmental Factors on the Growth of a Microalga Chlorella sp. UK 001, ” Proc. of 28 th Autumn Meeting of the SCEJ., vol. 1, p. 334, Sapporo, Japan (1995) – reference: Huang, L. and P. A. Castelfranco ; “Regulation of 5-Aminolevulinic Acid (ALA) Synthesis in Developing Chloroplasts. III. Evidence for Functional Heterogeneity of the ALA Pool, ” Plant Physiol., 92,172-178 (1990) – reference: Bandyopadhyay, B., A. E. Humphrey and H. Taguchi ; “Dynamic Measurement of the Volumetric Oxygen Transfer Coefficient in Fermentation Systems, ” Biotechnol. Bioeng., 9,533-544 (1967) – reference: Ogbonna, J. C., H. Yada and H. Tanaka ; “Effect of Cell Movement by Random Mixing Between the Surface and Bottom of Photobioreactor, ” J. Ferment. Bioeng., 79,152-157 (1995 a) – reference: Weinstein, J. D., R. W. Howell, R. D. Leverette, S. Y. Grooms, P. S. Brignola, S. M. Mayer and S. I. Beale ; “Heme Inhibition of δ-Aminolevulinic Acid Synthesis Is Enhanced by Glutathione in Cell-Free Extracts of Chlorella, ” Plant Physiol., 101,657-665 (1993) – reference: Endo, H., H. Hosoya and T. Koibuchi ; “Growth Yields of Chlorella regularis in Dark-heterotrophic Continuous Cultures Using Ace-tate, ” J. Ferment. Technol., 55,369-379 (1977) – reference: Tanaka, A., R. Tanaka, H. Ito, K. Yoshida ; “Kurorofiru No Seigousei, ” Tanpakushitsu Kakusan Kouso, 42,136-145 (1996) – reference: Ogbonna, J. C., H. Yada, H. Yasui and H. Tanaka ; “A Novel Internally Illuminated Stirred Tank Photobioreactor for Large-Scale Cultivation of Photosynthetic Cells, ” J. Ferment. Bioeng., 82, 61-67 (1996) – reference: Tigier, H. A., A. M. del C. Batlle and G. Locascio ; “Porphyrin Biosynthesis in Soybean Callus Tissue System. Isolation, Purification and General Properties of delta-Aminolevulinate Dehydratase, ” Biochim. Biophys. Acta, 15 300-302 (1968) – reference: Ogbonna, J. C., H. Yada and H. Tanaka ; “Light Supply Coefficient : a New Engineering Parameter for Photobioreactor Design, ” J. Ferment. Bioeng., 80,369-376 (1995 b) – reference: Hirata, S., M. Taya and S. Tone ; “Effect of Temperature on Change in Cell Diameter of Chlorella sp. UK 001 in Photoautotrophic Culture, ” Kagaku Kogaku Ronbunshu, 23,200-207 (1997) – reference: Comar, C. L. and F. P. Zscheile ; “Analysis of Plant Extracts for Chlorophylls a and b by a Photoelectric Spectrophotometric Method, ” Plant Physiol., 17,198-209 (1942) |
| SSID | ssib002484398 ssj0035378 ssib020472865 ssib000961565 ssib031741082 |
| Score | 1.4406013 |
| Snippet | Chlorella sp., which has been used for industrial biomass production, was cultured under a heterotrophic condition. When pH was kept at 6.0 during the initial... |
| SourceID | crossref jstage |
| SourceType | Enrichment Source Index Database Publisher |
| StartPage | 214 |
| SubjectTerms | biochemical engineering Chlorella sp chlorophyll biosynthesis heterotrophic culture |
| Title | Effects of pH Shift and Agitation Speed on Chlorophyll Biosynthesis in Heterotrophic Culture of Chlorella |
| URI | https://www.jstage.jst.go.jp/article/kakoronbunshu1975/25/2/25_2_214/_article/-char/en |
| Volume | 25 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | KAGAKU KOGAKU RONBUNSHU, 1999/03/10, Vol.25(2), pp.214-219 |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lj9MwELbKwgEOiKdYXvKB2ypL4ziNcwyrXVIiUok20nKqYidmu1ulFaQH-Kn8GsaPuIlYCVghVa6V2Fbs-TSeGdnfIPRGyLGUQow9zgTxqAh8L2a08jgASlKflFwH9D_mk7SgH87D89HoZ-_U0q7lx-LHtfdKbiJVeAZyVbdk_0GyblB4AHWQL5QgYSj_Ssan-8MY2_RofrGS5rx48sUyb6v08mBRQuXkAhzzDazpeq3yT3773oDlp8hINNcVLO6mVa9XwpJs1uacBnSqTVoiZ8JmyfskK46ymf77NMvfFfk8LRxy5kk-02FWqGUuJpurwNhnFc9XOr_Iposk24OnyJM0gQZ6I5wuZlB1HaGdHi9LFrAFTPdhCkVt4CCl4N6dQNVfbnkQOr5FA1ewDJqe6gvYxCO-znEIu5RRzQGNvZiMg77uNpemLUbJQBHT3p5u1fJv2wUJFf3sVXml2CL4rlF5Gkh47DoPiLitmJeD5n4chUsCP1v4dNm1U9fmAKW30G0SRb5mIE4HriO4zgNqOdqjYiSKxZPt34OZR_0xc9RoQRgYK6NbKXstFGb09rr5DMyuO5fgeXSnFrUhtXiA7lsPCCfm-x-i0WX5CN3r8WI-RisLbLyReJtiDWwMwMYO2FgDG0OlB2zcBzZeNXgAbGyBrQZ1wH6CirPTxUnq2ZwgnqCEth4NBZMsEgLMzjqsQlmVZcRJWIMpTGsaTXhVR5WQoGl4GUxiKoWMwprxmDDOwB5_ig6aTVM_Q5hGsQgiWbFa-dRS8rHwoSOL6SQmZRkeoqBbsqWws1N5W9ZL5TjDQg-RoAHg00PkuV5bQxjzh_ZnRhqu9Q1x9vx_DfQC3TXcJCrO-BIdtF939SuwvFv-WkP4FyDR3Q8 |
| linkProvider | ABC ChemistRy |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Effects+of+pH+Shift+and+Agitation+Speed+on+Chlorophyll+Biosynthesis+in+Heterotrophic+Culture+of+Chlorella&rft.jtitle=KAGAKU+KOGAKU+RONBUNSHU&rft.au=ASANO%2C+MASAKI&rft.au=NISHIYAMA%2C+YUKITAKA&rft.au=FUNAHASHI%2C+HITOSHI&rft.au=NAKAO%2C+KATSUMI&rft.date=1999&rft.pub=The+Society+of+Chemical+Engineers%2C+Japan&rft.issn=0386-216X&rft.eissn=1349-9203&rft.volume=25&rft.issue=2&rft.spage=214&rft.epage=219&rft_id=info:doi/10.1252%2Fkakoronbunshu.25.214&rft.externalDocID=article_kakoronbunshu1975_25_2_25_2_214_article_char_en |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0386-216X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0386-216X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0386-216X&client=summon |