Compressed sensing in fluorescence microscopy

• Published in: Progress in biophysics and molecular biology Vol. 168; pp. 66 - 80
• Main Authors: Calisesi, Gianmaria, Ghezzi, Alberto, Ancora, Daniele, D'Andrea, Cosimo, Valentini, Gianluca, Farina, Andrea, Bassi, Andrea
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2022
• Subjects: Algorithms; Animals; Biomedical imaging; Compressed sensing; Computational imaging; Fluorescence microscopy; Inverse problems; Magnetic Resonance Imaging; Microscopy, Fluorescence; Optical Imaging; Signal Processing, Computer-Assisted; Optical imaging; Inverse problems; Computational imaging; Compressed sensing; Fluorescence microscopy; Biomedical imaging
• ISSN: 0079-6107; 1873-1732; 1873-1732
• DOI: 10.1016/j.pbiomolbio.2021.06.004

Compressed sensing (CS) is a signal processing approach that solves ill-posed inverse problems, from under-sampled data with respect to the Nyquist criterium. CS exploits sparsity constraints based on the knowledge of prior information, relative to the structure of the object in the spatial or other domains. It is commonly used in image and video compression as well as in scientific and medical applications, including computed tomography and magnetic resonance imaging. In the field of fluorescence microscopy, it has been demonstrated to be valuable for fast and high-resolution imaging, from single-molecule localization, super-resolution to light-sheet microscopy. Furthermore, CS has found remarkable applications in the field of mesoscopic imaging, facilitating the study of small animals’ organs and entire organisms. This review article illustrates the working principles of CS, its implementations in optical imaging and discusses several relevant uses of CS in the field of fluorescence imaging from super-resolution microscopy to mesoscopy.