Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan

Brucellosis is an important bacterial zoonosis caused by and in Pakistan. The status of canine brucellosis caused by remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad ( = 87) and Bahawalpur ( = 94). Presence of antibo...

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Published inPathogens (Basel) Vol. 8; no. 4; p. 294
Main Authors Jamil, Tariq, Melzer, Falk, Khan, Iahtasham, Iqbal, Mudassar, Saqib, Muhammad, Hammad Hussain, Muhammad, Schwarz, Stefan, Neubauer, Heinrich
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 13.12.2019
MDPI
Subjects
Abortion
Age
Agglutination
Animal husbandry
Animal welfare
Animals
Antibodies
Antigens
Brucella
Brucellosis
Cattle
Dairy farms
Deoxyribonucleic acid
DNA
Dogs
Epidemiology
Infections
Livestock
Milk
Multivariate analysis
Pets
Polymerase chain reaction
Population
Real time
Serology
Vaccines
Zoonoses
Pakistan
Pakistan
Brucella abortus
Brucella canis
zoonosis
Dogs
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Abstract Brucellosis is an important bacterial zoonosis caused by and in Pakistan. The status of canine brucellosis caused by remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad ( = 87) and Bahawalpur ( = 94). Presence of antibodies against and was determined using the slow agglutination test (SAT) and ELISA, respectively. Real-time PCR was performed to detect and differentiate DNA at the species level. In Faisalabad, the serological prevalence was found to be 9.2% (8/87) and 10.3% (9/87) by SAT and ELISA, respectively. Only one of the ELISA positive samples (1.15%) yielded amplification for DNA. In Bahawalpur, 63.8% (60/94) samples were found positive by SAT; however, none of the samples was positive by ELISA or by real-time PCR. Location, age (≥1 year) and body condition (weak) were found to be associated with infection, whereas presence of wounds was found to be associated with infection only. These findings point towards a risk of transmission from dog to livestock and humans and vice versa. The study expects to draw the attention of concerned authorities towards infection prevention and animal welfare. This study warrants further epidemiological investigation on brucellosis in pet dogs and their owners. To the best of our knowledge, this is first ever report on and in dogs in Pakistan.
AbstractList Brucellosis is an important bacterial zoonosis caused by B. abortus and B. melitensis in Pakistan. The status of canine brucellosis caused by B. canis remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad ( n = 87) and Bahawalpur ( n = 94). Presence of antibodies against B. canis and B. abortus/B. melitensis was determined using the slow agglutination test (SAT) and ELISA, respectively. Real-time PCR was performed to detect and differentiate Brucella DNA at the species level. In Faisalabad, the serological prevalence was found to be 9.2% (8/87) and 10.3% (9/87) by SAT and ELISA, respectively. Only one of the ELISA positive samples (1.15%) yielded amplification for B. abortus DNA. In Bahawalpur, 63.8% (60/94) samples were found positive by SAT; however, none of the samples was positive by ELISA or by real-time PCR. Location, age (≥1 year) and body condition (weak) were found to be associated with B. canis infection, whereas presence of wounds was found to be associated with B. abortus infection only. These findings point towards a risk of transmission from dog to livestock and humans and vice versa. The study expects to draw the attention of concerned authorities towards infection prevention and animal welfare. This study warrants further epidemiological investigation on brucellosis in pet dogs and their owners. To the best of our knowledge, this is first ever report on B. canis and B. abortus in dogs in Pakistan.
Brucellosis is an important bacterial zoonosis caused by and in Pakistan. The status of canine brucellosis caused by remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad ( = 87) and Bahawalpur ( = 94). Presence of antibodies against and was determined using the slow agglutination test (SAT) and ELISA, respectively. Real-time PCR was performed to detect and differentiate DNA at the species level. In Faisalabad, the serological prevalence was found to be 9.2% (8/87) and 10.3% (9/87) by SAT and ELISA, respectively. Only one of the ELISA positive samples (1.15%) yielded amplification for DNA. In Bahawalpur, 63.8% (60/94) samples were found positive by SAT; however, none of the samples was positive by ELISA or by real-time PCR. Location, age (≥1 year) and body condition (weak) were found to be associated with infection, whereas presence of wounds was found to be associated with infection only. These findings point towards a risk of transmission from dog to livestock and humans and vice versa. The study expects to draw the attention of concerned authorities towards infection prevention and animal welfare. This study warrants further epidemiological investigation on brucellosis in pet dogs and their owners. To the best of our knowledge, this is first ever report on and in dogs in Pakistan.
Brucellosis is an important bacterial zoonosis caused by B. abortus and B. melitensis in Pakistan. The status of canine brucellosis caused by B. canis remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad (n = 87) and Bahawalpur (n = 94). Presence of antibodies against B. canis and B. abortus/B. melitensis was determined using the slow agglutination test (SAT) and ELISA, respectively. Real-time PCR was performed to detect and differentiate Brucella DNA at the species level. In Faisalabad, the serological prevalence was found to be 9.2% (8/87) and 10.3% (9/87) by SAT and ELISA, respectively. Only one of the ELISA positive samples (1.15%) yielded amplification for B. abortus DNA. In Bahawalpur, 63.8% (60/94) samples were found positive by SAT; however, none of the samples was positive by ELISA or by real-time PCR. Location, age (≥1 year) and body condition (weak) were found to be associated with B. canis infection, whereas presence of wounds was found to be associated with B. abortus infection only. These findings point towards a risk of transmission from dog to livestock and humans and vice versa. The study expects to draw the attention of concerned authorities towards infection prevention and animal welfare. This study warrants further epidemiological investigation on brucellosis in pet dogs and their owners. To the best of our knowledge, this is first ever report on B. canis and B. abortus in dogs in Pakistan.
Author Jamil, Tariq
Iqbal, Mudassar
Schwarz, Stefan
Khan, Iahtasham
Neubauer, Heinrich
Melzer, Falk
Saqib, Muhammad
Hammad Hussain, Muhammad
AuthorAffiliation 4 University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan; mudassar.iqbal@iub.edu.pk
5 Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad 38000, Pakistan; drsaqib_vet@hotmail.com
2 Institute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, Germany; stefan.schwarz@fu-berlin.de
1 Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany; falk.melzer@fli.de (F.M.); heinrich.neubauer@fli.de (H.N.)
6 1 Vance Street, Bardia 2565, Australia; m.hammad.hussain@gmail.com
3 Section of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, sub-campus Jhang, 12-Km Chiniot Road, Jhang 35200, Pakistan; iahtasham.khan@uvas.edu.pk
AuthorAffiliation_xml – name: 6 1 Vance Street, Bardia 2565, Australia; m.hammad.hussain@gmail.com
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– name: 2 Institute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, Germany; stefan.schwarz@fu-berlin.de
– name: 5 Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad 38000, Pakistan; drsaqib_vet@hotmail.com
– name: 1 Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany; falk.melzer@fli.de (F.M.); heinrich.neubauer@fli.de (H.N.)
– name: 4 University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan; mudassar.iqbal@iub.edu.pk
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/31847082$$D View this record in MEDLINE/PubMed
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Issue 4
Keywords Pakistan
Brucella abortus
Brucella canis
zoonosis
Dogs
Language English
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Snippet Brucellosis is an important bacterial zoonosis caused by and in Pakistan. The status of canine brucellosis caused by remains obscure. In total, 181 serum...
Brucellosis is an important bacterial zoonosis caused by B. abortus and B. melitensis in Pakistan. The status of canine brucellosis caused by B. canis remains...
Brucellosis is an important bacterial zoonosis caused by B. abortus and B. melitensis in Pakistan. The status of canine brucellosis caused by B. canis remains...
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StartPage 294
SubjectTerms Abortion
Age
Agglutination
Animal husbandry
Animal welfare
Animals
Antibodies
Antigens
Brucella
Brucellosis
Cattle
Dairy farms
Deoxyribonucleic acid
DNA
Dogs
Epidemiology
Infections
Livestock
Milk
Multivariate analysis
Pets
Polymerase chain reaction
Population
Real time
Serology
Vaccines
Zoonoses
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Title Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan
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