Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan
Brucellosis is an important bacterial zoonosis caused by and in Pakistan. The status of canine brucellosis caused by remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad ( = 87) and Bahawalpur ( = 94). Presence of antibo...
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Published in | Pathogens (Basel) Vol. 8; no. 4; p. 294 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
13.12.2019
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Brucellosis is an important bacterial zoonosis caused by
and
in Pakistan. The status of canine brucellosis caused by
remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad (
= 87) and Bahawalpur (
= 94). Presence of antibodies against
and
was determined using the slow agglutination test (SAT) and ELISA, respectively. Real-time PCR was performed to detect and differentiate
DNA at the species level. In Faisalabad, the serological prevalence was found to be 9.2% (8/87) and 10.3% (9/87) by SAT and ELISA, respectively. Only one of the ELISA positive samples (1.15%) yielded amplification for
DNA. In Bahawalpur, 63.8% (60/94) samples were found positive by SAT; however, none of the samples was positive by ELISA or by real-time PCR. Location, age (≥1 year) and body condition (weak) were found to be associated with
infection, whereas presence of wounds was found to be associated with
infection only. These findings point towards a risk of transmission from dog to livestock and humans and vice versa. The study expects to draw the attention of concerned authorities towards infection prevention and animal welfare. This study warrants further epidemiological investigation on brucellosis in pet dogs and their owners. To the best of our knowledge, this is first ever report on
and
in dogs in Pakistan. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2076-0817 2076-0817 |
DOI: | 10.3390/pathogens8040294 |