Expression and in situ processing of human prorenin to active renin in baculovirus-infected Sf-9 insect cell cultures under several infective conditions

• Published in: Biochemical engineering journal Vol. 43; no. 2; pp. 216 - 220
• Main Authors: Gotoh, Takeshi, Awa, Hirono, Kikuchi, Ken-Ichi, Takahashi, Saori
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.2009; Elsevier
• Subjects: Baculovirus; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Prorenin; Protease; Renin; Sf-9 insect cell; Sf-9 insect cell; Renin; Baculovirus; Protease; Prorenin; Human; Cell culture; Enzyme; In situ; Insecta; Virus; Infection; Peptidases; Baculoviridae; Arthropoda; Hydrolases; Aspartic endopeptidases; Invertebrata

In the baculovirus expression vector system (BEVS), intrinsic proteases concomitantly produced by infected insect cells have been generally regarded as a defect, because they sometimes degrade expressed recombinant proteins and decrease the productivity. The present study successfully used the prote...