Natural‐abundance isotope ratio mass spectrometry as a means of evaluating carbon redistribution during glucose–citrate cofermentation by Lactococcus lactis
The cometabolism of citrate and glucose by growing Lactococcus lactis ssp. lactis bv. diacetylactis was studied using a natural‐abundance stable isotope technique. By a judicious choice of substrates differing slightly in their 13C/12C ratios, the simultaneous metabolism of citrate and glucose to a...
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| Published in | European journal of biochemistry Vol. 271; no. 22; pp. 4392 - 4400 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Blackwell Science Ltd
01.11.2004
Blackwell Publishing Ltd |
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| Abstract | The cometabolism of citrate and glucose by growing Lactococcus lactis ssp. lactis bv. diacetylactis was studied using a natural‐abundance stable isotope technique. By a judicious choice of substrates differing slightly in their 13C/12C ratios, the simultaneous metabolism of citrate and glucose to a range of compounds was analysed. These end‐products include lactate, acetate, formate, diacetyl and acetoin. All these products have pyruvate as a common intermediate. With the objective of estimating the degree to which glucose and citrate metabolism through pyruvate may be differentially regulated, the δ13C values of the products accumulated over a wide range of concentrations of citrate and glucose were compared. It was found that, whereas the relative accumulation of different products responds to both the substrate concentration and the ratio between the substrates, the δ13C values of the products primarily reflect the availability of the two substrates over the entire range examined. It can be concluded that in actively growing L. lactis the maintenance of pyruvate homeostasis takes precedence over the redox status of the cells as a regulatory factor. |
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| AbstractList | The cometabolism of citrate and glucose by growing Lactococcus lactis ssp. lactis bv. diacetylactis was studied using a natural‐abundance stable isotope technique. By a judicious choice of substrates differing slightly in their 13C/12C ratios, the simultaneous metabolism of citrate and glucose to a range of compounds was analysed. These end‐products include lactate, acetate, formate, diacetyl and acetoin. All these products have pyruvate as a common intermediate. With the objective of estimating the degree to which glucose and citrate metabolism through pyruvate may be differentially regulated, the δ13C values of the products accumulated over a wide range of concentrations of citrate and glucose were compared. It was found that, whereas the relative accumulation of different products responds to both the substrate concentration and the ratio between the substrates, the δ13C values of the products primarily reflect the availability of the two substrates over the entire range examined. It can be concluded that in actively growing L. lactis the maintenance of pyruvate homeostasis takes precedence over the redox status of the cells as a regulatory factor. The cometabolism of citrate and glucose by growing Lactococcus lactis ssp. lactis bv. diacetylactis was studied using a natural-abundance stable isotope technique. By a judicious choice of substrates differing slightly in their 13C/12C ratios, the simultaneous metabolism of citrate and glucose to a range of compounds was analysed. These end-products include lactate, acetate, formate, diacetyl and acetoin. All these products have pyruvate as a common intermediate. With the objective of estimating the degree to which glucose and citrate metabolism through pyruvate may be differentially regulated, the delta13C values of the products accumulated over a wide range of concentrations of citrate and glucose were compared. It was found that, whereas the relative accumulation of different products responds to both the substrate concentration and the ratio between the substrates, the delta13C values of the products primarily reflect the availability of the two substrates over the entire range examined. It can be concluded that in actively growing L. lactis the maintenance of pyruvate homeostasis takes precedence over the redox status of the cells as a regulatory factor. The cometabolism of citrate and glucose by growing Lactococcus lactis ssp. lactis bv. diacetylactis was studied using a natural-abundance stable isotope technique. By a judicious choice of substrates differing slightly in their super(13)C/ super(12)C ratios, the simultaneous metabolism of citrate and glucose to a range of compounds was analysed. These end-products include lactate, acetate, formate, diacetyl and acetoin. All these products have pyruvate as a common intermediate. With the objective of estimating the degree to which glucose and citrate metabolism through pyruvate may be differentially regulated, the delta super(13)C values of the products accumulated over a wide range of concentrations of citrate and glucose were compared. It was found that, whereas the relative accumulation of different products responds to both the substrate concentration and the ratio between the substrates, the delta super(13)C values of the products primarily reflect the availability of the two substrates over the entire range examined. It can be concluded that in actively growing L. lactis the maintenance of pyruvate homeostasis takes precedence over the redox status of the cells as a regulatory factor. The cometabolism of citrate and glucose by growing Lactococcus lactis ssp. lactis bv. diacetylactis was studied using a natural‐abundance stable isotope technique. By a judicious choice of substrates differing slightly in their 13 C/ 12 C ratios, the simultaneous metabolism of citrate and glucose to a range of compounds was analysed. These end‐products include lactate, acetate, formate, diacetyl and acetoin. All these products have pyruvate as a common intermediate. With the objective of estimating the degree to which glucose and citrate metabolism through pyruvate may be differentially regulated, the δ 13 C values of the products accumulated over a wide range of concentrations of citrate and glucose were compared. It was found that, whereas the relative accumulation of different products responds to both the substrate concentration and the ratio between the substrates, the δ 13 C values of the products primarily reflect the availability of the two substrates over the entire range examined. It can be concluded that in actively growing L. lactis the maintenance of pyruvate homeostasis takes precedence over the redox status of the cells as a regulatory factor. |
| Author | Mahmoud, Mohamed Gentil, Emmanuel Robins, Richard J. |
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| Cites_doi | 10.1002/(SICI)1096-9888(199603)31:3<225::AID-JMS319>3.0.CO;2-L 10.1021/ac0013204 10.1002/recl.19590780703 10.1128/AEM.65.3.1202-1206.1999 10.1046/j.0014-2956.2001.02599.x 10.1099/00221287-148-4-1003 10.1017/S0022029900002880 10.1128/aem.57.11.3371-3377.1991 10.1007/BF00127882 10.1099/00221287-148-11-3467 10.1016/S0021-9258(18)47543-6 10.1042/bj2520913 10.3168/jds.S0022-0302(97)75981-2 10.1111/j.1365-3040.1992.tb01660.x 10.1007/BF00169412 10.3168/jds.S0022-0302(94)77232-5 10.1007/BF01570108 10.1007/BF00180151 10.1128/AEM.66.9.4112-4114.2000 10.1128/AEM.60.6.1739-1748.1994 10.1128/jb.174.14.4838-4841.1992 10.1023/A:1020643918089 10.1007/BF02919393 10.1128/JB.185.5.1564-1571.2003 10.1016/S0378-1097(99)00587-X 10.1007/BF00395936 10.1128/JB.181.5.1451-1457.1999 10.1074/jbc.M202573200 10.1128/AEM.66.12.5518-5520.2000 10.1046/j.1432-1327.2000.01424.x 10.3168/jds.S0022-0302(96)76425-1 10.1016/S0723-2020(99)80002-2 10.1128/AEM.69.6.3462-3468.2003 10.1021/jf9908852 10.1016/0168-6445(93)90062-E 10.1128/AEM.62.7.2641-2643.1996 10.1007/BF00167912 10.1016/S0958-6946(00)00103-5 10.1007/s00253-003-1313-8 10.1128/AEM.29.6.807-813.1975 10.1111/j.1365-2672.1993.tb02792.x 10.1002/bms.1200010609 10.1111/j.1574-6968.1995.tb07794.x 10.1016/S0958-1669(99)00016-6 10.1016/0304-4165(95)00103-4 |
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| Notes | Enzymes acetaldehyde dehydrogenase (EC 1.2.1.10); α‐acetolactate decarboxylase (EC 4.1.1.5); α‐acetolactate synthase (EC 2.2.1.6); acetyl kinase (EC 2.7.2.1); alcohol dehydrogenase (EC 1.1.1.1); citrate lyase (EC 4.1.3.6); diacetyl reductase (EC 1.1.1.5) lactate dehydrogenase (EC 1.1.1.27); phosphate acetyl transferase (EC 2.3.1.8); pyruvate dehydrogenase (acetyl‐transferring) complex (EC 1.2.4.1 + EC 2.3.1.12 + EC 1.8.1.4); pyruvate formate‐lyase (EC 2.3.1.54). l ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
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| References | 1997; 80 1987; 262 1996; 18 1990; 56 1991; 34 1991; 57 2000; 48 1995; 1245 2000; 66 2002; 277 1939; 10 1999; 22 1974; 1 1999; 65 2002; 82 2004 1996; 70 1992; 15 1992; 38 1995; 131 1996; 79 1994; 60 2001; 268 1996; 31 1998; 69 1993; 15 1993; 12 1992; 174 2000; 267 2000; 10 1993; 75 1999; 181 1975; 29 2000; 182 2003; 69 1994; 77 2002; 148 1999; 10 1996; 62 1988; 252 2003; 62 1989; 19 2001; 73 1959; 78 2003; 185 e_1_2_11_31_2 e_1_2_11_30_2 e_1_2_11_13_2 e_1_2_11_35_2 e_1_2_11_12_2 e_1_2_11_34_2 e_1_2_11_11_2 e_1_2_11_33_2 e_1_2_11_10_2 e_1_2_11_6_2 e_1_2_11_28_2 e_1_2_11_5_2 e_1_2_11_27_2 e_1_2_11_4_2 e_1_2_11_26_2 e_1_2_11_3_2 e_1_2_11_25_2 Melzer E. (e_1_2_11_43_2) 1987; 262 e_1_2_11_2_2 e_1_2_11_47_2 Curic M. (e_1_2_11_14_2) 1999; 65 e_1_2_11_48_2 Crow V. (e_1_2_11_32_2) 1990; 56 e_1_2_11_29_2 e_1_2_11_20_2 e_1_2_11_44_2 e_1_2_11_45_2 e_1_2_11_46_2 e_1_2_11_24_2 e_1_2_11_9_2 e_1_2_11_23_2 e_1_2_11_40_2 e_1_2_11_8_2 e_1_2_11_22_2 e_1_2_11_41_2 e_1_2_11_7_2 e_1_2_11_21_2 e_1_2_11_42_2 e_1_2_11_17_2 e_1_2_11_16_2 Verhue W. (e_1_2_11_18_2) 1991; 57 e_1_2_11_15_2 e_1_2_11_36_2 e_1_2_11_37_2 e_1_2_11_19_2 e_1_2_11_38_2 e_1_2_11_39_2 |
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| Snippet | The cometabolism of citrate and glucose by growing Lactococcus lactis ssp. lactis bv. diacetylactis was studied using a natural‐abundance stable isotope... The cometabolism of citrate and glucose by growing Lactococcus lactis ssp. lactis bv. diacetylactis was studied using a natural-abundance stable isotope... The cometabolism of citrate and glucose by growing Lactococcus lactis ssp. lactis bv. diacetylactis was studied using a natural‐abundance stable isotope... |
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| SubjectTerms | Carbon - analysis Carbon - metabolism carbon balance Carbon Isotopes Citrates - metabolism Fermentation Glucose - metabolism isotope ratio mass spectrometry lactic acid bacteria Lactococcus lactis Lactococcus lactis - metabolism Mass Spectrometry - methods metabolic regulation pyruvate Pyruvic Acid - metabolism |
| Title | Natural‐abundance isotope ratio mass spectrometry as a means of evaluating carbon redistribution during glucose–citrate cofermentation by Lactococcus lactis |
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