A novel environmental chamber for neuronal network multisite recordings

• Published in: Biotechnology and bioengineering Vol. 109; no. 10; pp. 2553 - 2566
• Main Authors: Biffi, E., Regalia, G., Ghezzi, D., De Ceglia, R., Menegon, A., Ferrigno, G., Fiore, G.B., Pedrocchi, A.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.10.2012; Wiley Subscription Services, Inc
• Subjects: Animals; Cell culture; Cell Culture Techniques; Design of experiments; Electronics - methods; Electrophysiological Phenomena; Experimental design; Humidity; Hydrogen-Ion Concentration; hyperosmolarity; Incubators; long-term recording; Mice; Micro Electrode Arrays; neuronal cell culture; Neurons; Neurons - physiology; Neurosciences; Organ Culture Techniques - methods; Physiology; Temperature; temperature control

Environmental stability is a critical issue for neuronal networks in vitro. Hence, the ability to control the physical and chemical environment of cell cultures during electrophysiological measurements is an important requirement in the experimental design. In this work, we describe the development...