A novel environmental chamber for neuronal network multisite recordings
Environmental stability is a critical issue for neuronal networks in vitro. Hence, the ability to control the physical and chemical environment of cell cultures during electrophysiological measurements is an important requirement in the experimental design. In this work, we describe the development...
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| Published in | Biotechnology and bioengineering Vol. 109; no. 10; pp. 2553 - 2566 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
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01.10.2012
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| Abstract | Environmental stability is a critical issue for neuronal networks in vitro. Hence, the ability to control the physical and chemical environment of cell cultures during electrophysiological measurements is an important requirement in the experimental design. In this work, we describe the development and the experimental verification of a closed chamber for multisite electrophysiology and optical monitoring. The chamber provides stable temperature, pH and humidity and guarantees cell viability comparable to standard incubators. Besides, it integrates the electronics for long‐term neuronal activity recording. The system is portable and adaptable for multiple network housings, which allows performing parallel experiments in the same environment. Our results show that this device can be a solution for long‐term electrophysiology, for dual network experiments and for coupled optical and electrical measurements. Biotechnol. Bioeng. 2012; 109: 2553–2566. © 2012 Wiley Periodicals, Inc.
The authors describe the development and the experimental verification of a closed chamber for multisite electrophysiology and optical monitoring. The chamber provides stable temperature, pH, and humidity and guarantees cell viability comparable to standard incubators. It also integrates the electronics for long‐term neuronal activity recording. This device is a solution for long‐term electrophysiology, for dual network experiments and for coupled optical and electrical measurements. |
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| AbstractList | Environmental stability is a critical issue for neuronal networks in vitro. Hence, the ability to control the physical and chemical environment of cell cultures during electrophysiological measurements is an important requirement in the experimental design. In this work, we describe the development and the experimental verification of a closed chamber for multisite electrophysiology and optical monitoring. The chamber provides stable temperature, pH and humidity and guarantees cell viability comparable to standard incubators. Besides, it integrates the electronics for long-term neuronal activity recording. The system is portable and adaptable for multiple network housings, which allows performing parallel experiments in the same environment. Our results show that this device can be a solution for long-term electrophysiology, for dual network experiments and for coupled optical and electrical measurements. Environmental stability is a critical issue for neuronal networks in vitro. Hence, the ability to control the physical and chemical environment of cell cultures during electrophysiological measurements is an important requirement in the experimental design. In this work, we describe the development and the experimental verification of a closed chamber for multisite electrophysiology and optical monitoring. The chamber provides stable temperature, pH and humidity and guarantees cell viability comparable to standard incubators. Besides, it integrates the electronics for long‐term neuronal activity recording. The system is portable and adaptable for multiple network housings, which allows performing parallel experiments in the same environment. Our results show that this device can be a solution for long‐term electrophysiology, for dual network experiments and for coupled optical and electrical measurements. Biotechnol. Bioeng. 2012; 109: 2553–2566. © 2012 Wiley Periodicals, Inc. The authors describe the development and the experimental verification of a closed chamber for multisite electrophysiology and optical monitoring. The chamber provides stable temperature, pH, and humidity and guarantees cell viability comparable to standard incubators. It also integrates the electronics for long‐term neuronal activity recording. This device is a solution for long‐term electrophysiology, for dual network experiments and for coupled optical and electrical measurements. Environmental stability is a critical issue for neuronal networks in vitro. Hence, the ability to control the physical and chemical environment of cell cultures during electrophysiological measurements is an important requirement in the experimental design. In this work, we describe the development and the experimental verification of a closed chamber for multisite electrophysiology and optical monitoring. The chamber provides stable temperature, pH and humidity and guarantees cell viability comparable to standard incubators. Besides, it integrates the electronics for long-term neuronal activity recording. The system is portable and adaptable for multiple network housings, which allows performing parallel experiments in the same environment. Our results show that this device can be a solution for long-term electrophysiology, for dual network experiments and for coupled optical and electrical measurements. [PUBLICATION ABSTRACT] Abstract Environmental stability is a critical issue for neuronal networks in vitro. Hence, the ability to control the physical and chemical environment of cell cultures during electrophysiological measurements is an important requirement in the experimental design. In this work, we describe the development and the experimental verification of a closed chamber for multisite electrophysiology and optical monitoring. The chamber provides stable temperature, pH and humidity and guarantees cell viability comparable to standard incubators. Besides, it integrates the electronics for long‐term neuronal activity recording. The system is portable and adaptable for multiple network housings, which allows performing parallel experiments in the same environment. Our results show that this device can be a solution for long‐term electrophysiology, for dual network experiments and for coupled optical and electrical measurements. Biotechnol. Bioeng. 2012; 109: 2553–2566. © 2012 Wiley Periodicals, Inc. |
| Author | Ghezzi, D. Ferrigno, G. Menegon, A. Biffi, E. De Ceglia, R. Regalia, G. Fiore, G.B. Pedrocchi, A. |
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| Cites_doi | 10.1016/j.neuro.2010.04.001 10.1111/j.1460-9568.2008.06259.x 10.1109/TBME.2004.827936 10.1007/s12038-009-0009-3 10.2144/03354bi01 10.1016/j.neulet.2003.12.062 10.1016/S0956-5663(03)00092-7 10.1016/S0165-022X(01)00163-4 10.1016/j.jphysparis.2005.12.005 10.1109/10.936367 10.1083/jcb.32.1.89 10.1002/bit.23310 10.1007/BF02623645 10.3389/neuro.16.012.2009 10.1007/BF00584664 10.1109/TBME.2005.856245 10.1016/S0165-0270(01)00412-5 10.1016/0165-0270(88)90116-1 10.1155/2010/659050 10.1002/eej.10216 10.1016/S0956-5663(00)00095-6 10.1016/0165-0270(94)90059-0 10.1016/0014-4827(53)90089-5 10.1016/j.bios.2010.01.001 10.1007/s10544-009-9332-6 10.2144/05382RR03 10.1371/journal.pone.0020198 10.1124/jpet.108.144444 10.1523/JNEUROSCI.21-22-08782.2001 10.1263/jbb.100.131 10.1016/j.brainres.2006.03.049 10.1152/ajpcell.1989.256.1.C214 10.3181/00379727-100-24546 |
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| References | Howell KR, Kutiyanawalla A, Pillai A. 2011. Long-term continuous corticosterone treatment decreases VEGF receptor-2 expression in frontal cortex. PLoS ONE 6(5): e20198. Bai Q, Wise K. 2001. Single-unit neural recording with active microelectrode arrays. IEEE Trans Biomed Eng 48: 911-920. Gross G, Schwalm F. 1994. A closed flow chamber for long-term multichannel recording and optical monitoring. J Neurosci Methods 52: 73-85. Vukasinovic J, Cullen D, LaPlaca M, Glezer A. 2009. A microperfused incubator for tissue mimetic 3D cultures. Biomed Microdevices 11: 1155-1165. Morefield S, Keefer E, Chapman KD, Gross GW. 2000. Drug evaluations using neuronal networks cultured on microelectrode arrays. Biosens Bioelectron 15: 383-396. Forsythe I, Coates R. 1988. A chamber for electrophysiological recording from cultured neurones allowing perfusion and temperature control. J Neurosci Methods 25: 19-27. Heidemann S, Lamoureux P, Ngo K, Reynolds M, Buxbaum R. 2003. Open-dish incubator for live cell imaging with an inverted microscope. Biotechniques 35: 708-714, 716. Biffi E, Ghezzi D, Pedrocchi A, Ferrigno G. 2010. Development and validation of a spike detection and classification algorithm aimed at implementation on hardware devices. Comput Intell Neurosci 2010: 659050. Mukai J, Shiina T, Jimbo Y. 2003. Continuous monitoring of developmental activity changes in cultured cortical networks. Electr Eng Jpn 145: 28-37. Rose G. 1967. The circumfusion system for multipurpose culture chambers. I. Introduction to the mechanics, techniques, and basic results of a 12-chamber (in vitro) closed circulatory system. J Cell Biol 32: 89-112. Chiappalone M, Bove M, Vato A, Tedesco M, Martinoia S. 2006. Dissociated cortical networks show spontaneously correlated activity patterns during in vitro development. Brain Res 1093: 41-53. Blau A, Ziegler C. 2001. Prototype of a novel autonomous perfusion chamber for long-term culturing and in situ investigation of various cell types. J Biochem Biophys Methods 50: 15-27. Johnstone A, Gross G, Weiss D, Schroeder O, Gramowski A, Shafer T. 2010. Microelectrode arrays: A physiologically based neurotoxicity testing platform for the 21st century. Neurotoxicology 31: 331-350. Hofmann F, Bading H. 2006. Long term recordings with microelectrode arrays: Studies of transcription-dependent neuronal plasticity and axonal regeneration. J Physiol Paris 99: 125-132. Blau A, Neumann T, Ziegler C, Benfenati F. 2009. Replica-moulded polydimethylsiloxane culture vessel lids attenuate osmotic drift in long-term cell cultures. J Biosci 34: 59-69. Xiang K, Earl DE, Davis KM, Giovannucci DR, Greenfield LJ, Jr., Tietz EI. 2008. Chronic benzodiazepine administration potentiates high voltage-activated calcium currents in hippocampal CA1 neurons. J Pharmacol Exp Ther 327: 872-883. Sykes J, Moore E. 1959. A new chamber for tissue culture. Proc Soc Exp Biol Med 100: 125-127. Pancrazio J, Gray S, Shubin Y, Kulagina N, Cuttino D, Shaffer K, Eisemann K, Curran A, Zim B, Gross GW, O'Shaughnessy TJ. 2003. A portable microelectrode array recording system incorporating cultured neuronal networks for neurotoxin detection. Biosens Bioelectron 18: 1339-1347. Fantini E, Athias P, Courtois M, Grynberg A. 1987. A simple gas-flow chamber for cultured cell electrophysiology in a controlled atmosphere. Pflugers Arch 409: 632-634. Shahaf G, Marom S. 2001. Learning in networks of cortical neurons. J Neurosci 21: 8782-8788. Bavister B. 1988. A minichamber device for maintaining a constant carbon dioxide in air atmosphere during prolonged culture of cells on the stage of an inverted microscope. In Vitro Cell Dev Biol 24: 759-763. Hales C, Rolston J, Potter S. 2010. How to culture, record and stimulate neuronal networks on micro-electrode arrays (MEAs). J Vis Exp 39: 2056. Toyotomi S, Momose Y. 1989. Temperature-controlled perfusion apparatus for microscope using transparent conducting film heater. Am J Physiol 256: C214-C217. Merril DR, Tresco PA. 2005. Impedance characterization of microarray recording electrodes in vitro. IEEE Trans Biomed Eng 52: 1960-1965. van Pelt J, Corner M, Wolters P, Rutten W, Ramakers G. 2004a. Longterm stability and developmental changes in spontaneous network burst firing patterns in dissociated rat cerebral cortex cell cultures on multielectrode arrays. Neurosci Lett 361: 86-89. Biffi E, Menegon A, Piraino F, Pedrocchi A, Fiore GB, Rasponi M. 2011. Validation of long-term primary neuronal cultures and network activity through the integration of reversibly bonded microbioreactors and MEA substrates. Biotechnol Bioeng 109: 166-175. Morin F, Takamura Y, Tamiya E. 2005. Investigating neuronal activity with planar microelectrode arrays: Achievements and new perspectives. J Biosci Bioeng 100: 131-143. Christiansen G, Danes B, Allen L, Leinfelder P. 1953. A culture chamber for the continuous biochemical and morphological study of living cells in tissue culture. Exp Cell Res 5: 10-15. Chiappalone M, Massobrio P, Martinoia S. 2008. Network plasticity in cortical assemblies. Eur J Neurosci 28: 221-237. Rolston JD, Gross RE, Potter SM. 2009. A low-cost multielectrode system for data acquisition enabling real-time closed-loop processing with rapid recovery from stimulation artifacts. Front Neuroeng 2: 1-17. Charvet G, Rousseau L, Billoint O, Gharbi S, Rostaing JP, Joucla S, Trevisiol M, Bourgerette A, Chauvet P, Moulin C, Goy F, Mercier B, Colin M, Spirkovitch S, Fanet H, Meyrand P, Guillemaud R, Yvert B. 2010. BioMEA: A versatile high-density 3D microelectrode array system using integrated electronics. Biosens Bioelectron 25: 1889-1896. Potter S, DeMarse T. 2001. A new approach to neural cell culture for long-term studies. J Neurosci Methods 110: 17-24. Ho C, Mou T, Chiang P, Weng C, Chow N. 2005. Mini chamber system for long-term maintenance and observation of cultured cells. Biotechniques 38: 267-273. van Pelt J, Wolters P, Corner M, Rutten W, Ramakers G. 2004b. Long-term characterization of firing dynamics of spontaneous bursts in cultured neural networks. IEEE Trans Biomed Eng 51: 2051-2062. 2001; 50 2010; 31 2010; 2010 1989; 256 2010; 39 2006; 99 2003; 35 1953; 5 2008; 327 1987; 409 2001; 48 2003; 18 2011; 6 2001; 21 2009; 34 2001; 110 2009; 11 2011; 109 2010; 25 2000; 15 2005; 100 1967; 32 1988; 25 2008; 28 2005; 52 1988; 24 2004a; 361 2005; 38 2009; 2 1959; 100 2003; 145 1994; 52 2004b; 51 2006; 1093 e_1_2_7_6_1 e_1_2_7_5_1 e_1_2_7_4_1 e_1_2_7_3_1 e_1_2_7_9_1 Hales C (e_1_2_7_15_1) 2010; 39 e_1_2_7_8_1 e_1_2_7_7_1 e_1_2_7_19_1 e_1_2_7_18_1 e_1_2_7_17_1 e_1_2_7_16_1 e_1_2_7_2_1 e_1_2_7_14_1 e_1_2_7_13_1 e_1_2_7_12_1 e_1_2_7_11_1 e_1_2_7_10_1 e_1_2_7_26_1 e_1_2_7_27_1 e_1_2_7_28_1 e_1_2_7_29_1 Toyotomi S (e_1_2_7_31_1) 1989; 256 e_1_2_7_30_1 e_1_2_7_25_1 e_1_2_7_24_1 e_1_2_7_32_1 e_1_2_7_23_1 e_1_2_7_33_1 e_1_2_7_22_1 e_1_2_7_34_1 e_1_2_7_21_1 e_1_2_7_35_1 e_1_2_7_20_1 |
| References_xml | – volume: 1093 start-page: 41 year: 2006 end-page: 53 article-title: Dissociated cortical networks show spontaneously correlated activity patterns during development publication-title: Brain Res – volume: 21 start-page: 8782 year: 2001 end-page: 8788 article-title: Learning in networks of cortical neurons publication-title: J Neurosci – volume: 50 start-page: 15 year: 2001 end-page: 27 article-title: Prototype of a novel autonomous perfusion chamber for long‐term culturing and in situ investigation of various cell types publication-title: J Biochem Biophys Methods – volume: 28 start-page: 221 year: 2008 end-page: 237 article-title: Network plasticity in cortical assemblies publication-title: Eur J Neurosci – volume: 52 start-page: 73 year: 1994 end-page: 85 article-title: A closed flow chamber for long‐term multichannel recording and optical monitoring publication-title: J Neurosci Methods – volume: 2 start-page: 1 year: 2009 end-page: 17 article-title: A low‐cost multielectrode system for data acquisition enabling real‐time closed‐loop processing with rapid recovery from stimulation artifacts publication-title: Front Neuroeng – volume: 2010 start-page: 659050 year: 2010 article-title: Development and validation of a spike detection and classification algorithm aimed at implementation on hardware devices publication-title: Comput Intell Neurosci – volume: 39 start-page: 2056 year: 2010 article-title: How to culture, record and stimulate neuronal networks on micro‐electrode arrays (MEAs) publication-title: J Vis Exp – volume: 35 start-page: 708 year: 2003 end-page: 714 article-title: Open‐dish incubator for live cell imaging with an inverted microscope publication-title: Biotechniques – volume: 18 start-page: 1339 year: 2003 end-page: 1347 article-title: A portable microelectrode array recording system incorporating cultured neuronal networks for neurotoxin detection publication-title: Biosens Bioelectron – volume: 110 start-page: 17 year: 2001 end-page: 24 article-title: A new approach to neural cell culture for long‐term studies publication-title: J Neurosci Methods – volume: 11 start-page: 1155 year: 2009 end-page: 1165 article-title: A microperfused incubator for tissue mimetic 3D cultures publication-title: Biomed Microdevices – volume: 409 start-page: 632 year: 1987 end-page: 634 article-title: A simple gas‐flow chamber for cultured cell electrophysiology in a controlled atmosphere publication-title: Pflugers Arch – volume: 5 start-page: 10 year: 1953 end-page: 15 article-title: A culture chamber for the continuous biochemical and morphological study of living cells in tissue culture publication-title: Exp Cell Res – volume: 25 start-page: 1889 year: 2010 end-page: 1896 article-title: BioMEA: A versatile high‐density 3D microelectrode array system using integrated electronics publication-title: Biosens Bioelectron – volume: 24 start-page: 759 year: 1988 end-page: 763 article-title: A minichamber device for maintaining a constant carbon dioxide in air atmosphere during prolonged culture of cells on the stage of an inverted microscope publication-title: In Vitro Cell Dev Biol – volume: 52 start-page: 1960 year: 2005 end-page: 1965 article-title: Impedance characterization of microarray recording electrodes in vitro publication-title: IEEE Trans Biomed Eng – volume: 48 start-page: 911 year: 2001 end-page: 920 article-title: Single‐unit neural recording with active microelectrode arrays publication-title: IEEE Trans Biomed Eng – volume: 51 start-page: 2051 year: 2004b end-page: 2062 article-title: Long‐term characterization of firing dynamics of spontaneous bursts in cultured neural networks publication-title: IEEE Trans Biomed Eng – volume: 100 start-page: 125 year: 1959 end-page: 127 article-title: A new chamber for tissue culture publication-title: Proc Soc Exp Biol Med – volume: 6 start-page: e20198 issue: 5 year: 2011 article-title: Long‐term continuous corticosterone treatment decreases VEGF receptor‐2 expression in frontal cortex publication-title: PLoS ONE – volume: 38 start-page: 267 year: 2005 end-page: 273 article-title: Mini chamber system for long‐term maintenance and observation of cultured cells publication-title: Biotechniques – volume: 109 start-page: 166 year: 2011 end-page: 175 article-title: Validation of long‐term primary neuronal cultures and network activity through the integration of reversibly bonded microbioreactors and MEA substrates publication-title: Biotechnol Bioeng – volume: 34 start-page: 59 year: 2009 end-page: 69 article-title: Replica‐moulded polydimethylsiloxane culture vessel lids attenuate osmotic drift in long‐term cell cultures publication-title: J Biosci – volume: 25 start-page: 19 year: 1988 end-page: 27 article-title: A chamber for electrophysiological recording from cultured neurones allowing perfusion and temperature control publication-title: J Neurosci Methods – volume: 99 start-page: 125 year: 2006 end-page: 132 article-title: Long term recordings with microelectrode arrays: Studies of transcription‐dependent neuronal plasticity and axonal regeneration publication-title: J Physiol Paris – volume: 31 start-page: 331 year: 2010 end-page: 350 article-title: Microelectrode arrays: A physiologically based neurotoxicity testing platform for the 21st century publication-title: Neurotoxicology – volume: 256 start-page: C214 year: 1989 end-page: C217 article-title: Temperature‐controlled perfusion apparatus for microscope using transparent conducting film heater publication-title: Am J Physiol – volume: 145 start-page: 28 year: 2003 end-page: 37 article-title: Continuous monitoring of developmental activity changes in cultured cortical networks publication-title: Electr Eng Jpn – volume: 100 start-page: 131 year: 2005 end-page: 143 article-title: Investigating neuronal activity with planar microelectrode arrays: Achievements and new perspectives publication-title: J Biosci Bioeng – volume: 15 start-page: 383 year: 2000 end-page: 396 article-title: Drug evaluations using neuronal networks cultured on microelectrode arrays publication-title: Biosens Bioelectron – volume: 361 start-page: 86 year: 2004a end-page: 89 article-title: Longterm stability and developmental changes in spontaneous network burst firing patterns in dissociated rat cerebral cortex cell cultures on multielectrode arrays publication-title: Neurosci Lett – volume: 32 start-page: 89 year: 1967 end-page: 112 article-title: The circumfusion system for multipurpose culture chambers. I. Introduction to the mechanics, techniques, and basic results of a 12‐chamber ( ) closed circulatory system publication-title: J Cell Biol – volume: 327 start-page: 872 year: 2008 end-page: 883 article-title: Chronic benzodiazepine administration potentiates high voltage‐activated calcium currents in hippocampal CA1 neurons publication-title: J Pharmacol Exp Ther – volume: 39 start-page: 2056 year: 2010 ident: e_1_2_7_15_1 article-title: How to culture, record and stimulate neuronal networks on micro‐electrode arrays (MEAs) publication-title: J Vis Exp contributor: fullname: Hales C – ident: e_1_2_7_20_1 doi: 10.1016/j.neuro.2010.04.001 – ident: e_1_2_7_10_1 doi: 10.1111/j.1460-9568.2008.06259.x – ident: e_1_2_7_33_1 doi: 10.1109/TBME.2004.827936 – ident: e_1_2_7_7_1 doi: 10.1007/s12038-009-0009-3 – ident: e_1_2_7_16_1 doi: 10.2144/03354bi01 – ident: e_1_2_7_32_1 doi: 10.1016/j.neulet.2003.12.062 – ident: e_1_2_7_25_1 doi: 10.1016/S0956-5663(03)00092-7 – ident: e_1_2_7_6_1 doi: 10.1016/S0165-022X(01)00163-4 – ident: e_1_2_7_18_1 doi: 10.1016/j.jphysparis.2005.12.005 – ident: e_1_2_7_2_1 doi: 10.1109/10.936367 – ident: e_1_2_7_28_1 doi: 10.1083/jcb.32.1.89 – ident: e_1_2_7_5_1 doi: 10.1002/bit.23310 – ident: e_1_2_7_3_1 doi: 10.1007/BF02623645 – ident: e_1_2_7_27_1 doi: 10.3389/neuro.16.012.2009 – ident: e_1_2_7_12_1 doi: 10.1007/BF00584664 – ident: e_1_2_7_21_1 doi: 10.1109/TBME.2005.856245 – ident: e_1_2_7_26_1 doi: 10.1016/S0165-0270(01)00412-5 – ident: e_1_2_7_13_1 doi: 10.1016/0165-0270(88)90116-1 – ident: e_1_2_7_4_1 doi: 10.1155/2010/659050 – ident: e_1_2_7_24_1 doi: 10.1002/eej.10216 – ident: e_1_2_7_22_1 doi: 10.1016/S0956-5663(00)00095-6 – ident: e_1_2_7_14_1 doi: 10.1016/0165-0270(94)90059-0 – ident: e_1_2_7_11_1 doi: 10.1016/0014-4827(53)90089-5 – ident: e_1_2_7_8_1 doi: 10.1016/j.bios.2010.01.001 – ident: e_1_2_7_34_1 doi: 10.1007/s10544-009-9332-6 – ident: e_1_2_7_17_1 doi: 10.2144/05382RR03 – ident: e_1_2_7_19_1 doi: 10.1371/journal.pone.0020198 – ident: e_1_2_7_35_1 doi: 10.1124/jpet.108.144444 – ident: e_1_2_7_29_1 doi: 10.1523/JNEUROSCI.21-22-08782.2001 – ident: e_1_2_7_23_1 doi: 10.1263/jbb.100.131 – ident: e_1_2_7_9_1 doi: 10.1016/j.brainres.2006.03.049 – volume: 256 start-page: C214 year: 1989 ident: e_1_2_7_31_1 article-title: Temperature‐controlled perfusion apparatus for microscope using transparent conducting film heater publication-title: Am J Physiol doi: 10.1152/ajpcell.1989.256.1.C214 contributor: fullname: Toyotomi S – ident: e_1_2_7_30_1 doi: 10.3181/00379727-100-24546 |
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| Title | A novel environmental chamber for neuronal network multisite recordings |
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