Adherence influences monocyte responsiveness to interleukin-10

• Published in: Journal of leukocyte biology Vol. 73; no. 1; pp. 145 - 154
• Main Authors: Petit-Bertron, A-F, Fitting, C, Cavaillon, J-M, Adib-Conquy, M
• Format: Journal Article
• Language: English
• Published: United States Society for Leukocyte Biology 01.01.2003
• Subjects: Antigens, CD - drug effects; Cell Adhesion - physiology; cytokines; Cytokines - drug effects; Cytokines - metabolism; DNA-Binding Proteins - drug effects; Drosophila Proteins; endotoxin; Heme Oxygenase (Decyclizing) - drug effects; Heme Oxygenase-1; HLA‐DR; human; Humans; inflammation; Interleukin-10 - pharmacology; Membrane Glycoproteins - drug effects; Membrane Glycoproteins - metabolism; Membrane Proteins; Monocytes - cytology; Monocytes - drug effects; Monocytes - metabolism; monocytes/macrophages; Phagocytosis - drug effects; protein kinase; Protein-Tyrosine Kinases; Proteins - drug effects; Receptors, Cell Surface - drug effects; Receptors, Cell Surface - metabolism; Repressor Proteins; signal transduction; Signal Transduction - drug effects; STAT3 Transcription Factor; Suppressor of Cytokine Signaling 3 Protein; Suppressor of Cytokine Signaling Proteins; Toll-Like Receptor 2; Toll-Like Receptor 4; Toll-Like Receptors; Trans-Activators - drug effects; Transcription Factors; TYK2 Kinase
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1189/jlb.0802388

We studied the effects of adherence on the properties of interleukin (IL)‐10 on monocyte‐enriched peripheral blood mononuclear cells. We found that the decrease of CD11b expression induced by IL‐10 was enhanced by adherence. Toll‐like receptor (TLR)2 and TLR4 mRNA, as well as TLR4 surface expression, were significantly up‐regulated by IL‐10 in adherent cells. The absence of adherence prevented the inhibitory effects of IL‐10 on lipopolysaccharide‐induced tumor necrosis factor (TNF) and granulocyte‐colony stimulating factor production and increased IL‐1β production and soluble TNF receptor II release in IL‐10‐pretreated cells. Similarly, the absence of adherence amplified the enhancement of phagocytosis induced by IL‐10. Tyk2 and signal transducer and activator of transcription 3 (STAT3) phosphorylation and suppressr of cytokine signaling 3 (SOCS3) expression were induced by IL‐10 in both conditions, but a longer activation and/or expression were observed in adherent monocytes. Finally, heme oxygenase‐1, an anti‐inflammatory molecule, was induced by IL‐10 in adherent monocytes, whereas its expression remained low in nonadherent cells. Altogether, these data illustrate that adherence modulates the properties and the anti‐inflammatory effects of IL‐10.