Pulvomycin‐resistant mutants of E.coli elongation factor Tu

• Published in: The EMBO journal Vol. 13; no. 21; pp. 5113 - 5120
• Main Authors: Zeef, L.A., Bosch, L., Anborgh, P.H., Cetin, R., Parmeggiani, A., Hilgenfeld, R.
• Format: Journal Article
• Language: English
• Published: England 01.11.1994
• Subjects: Aminoglycosides; Anti-Bacterial Agents - pharmacology; Binding Sites - genetics; Cell Membrane Permeability; Dose-Response Relationship, Drug; Drug Resistance, Microbial; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - genetics; Genes, Bacterial - genetics; Guanosine Triphosphate - chemistry; Models, Biological; Models, Molecular; Molecular Conformation; Mutagenesis, Site-Directed; Peptide Biosynthesis; Peptide Elongation Factor Tu - drug effects; Peptides; Protein Biosynthesis - drug effects; Pyridones - pharmacology; RNA, Transfer, Amino Acyl - metabolism; RNA, Transfer, Phe - metabolism; Selection, Genetic; Structure-Activity Relationship

This paper reports the generation of Escherichia coli mutants resistant to pulvomycin. Together with targeted mutagenesis of the tufA gene, conditions were found to overcome membrane impermeability, thereby allowing the selection of three mutants harbouring elongation factor (EF)‐Tu Arg230‐‐>Cys,...