The use of hyaluronan and its sulphated derivative patterned with micrometric scale on glass substrate in melanocyte cell behaviour
Surface microfabrication techniques were widely utilised for the spatial control of in vitro cell behaviour. A photo-immobilisation procedure was utilised to create micropatterned surfaces: four different stripe patterns (100, 50, 25 and 10 μm) of hyaluronan (Hyal) and its sulphated derivative (Hyal...
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Published in | Biomaterials Vol. 24; no. 6; pp. 915 - 926 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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Elsevier Ltd
01.03.2003
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Abstract | Surface microfabrication techniques were widely utilised for the spatial control of in vitro cell behaviour. A photo-immobilisation procedure was utilised to create micropatterned surfaces: four different stripe patterns (100, 50, 25 and 10
μm) of hyaluronan (Hyal) and its sulphated derivative (HyalS) on silanised glass substrate were obtained.
The morphological analysis showed that the surface topography showed regular stripes of 100, 50, 25 and 10
μm wide and ranging from 300
nm up to 1
μm in thickness. They reproduced the exact photo-mask pattern: glass stripes alternating with polysaccharide ones. On the contrary, Hyal microstructures showed just a topographic pattern as the glass stripes appeared to be covered by a thin layer of the macromolecule by TOF-SIMS.
Cell adhesion studies demonstrated that melanocytes adhered and oriented within the first 2
h of culture on HyalS microdomains and not on Hyal microstructures where they spread on glass substrate around the patterned area.
Double photo-immobilised samples characterised by a 100
μm stripe pattern of Hyal or HyalS on the top of a continuous layer of the two polysaccharides were also created in order to investigate the effect of the topography on cell behaviour. The obtained results demonstrated that melanocytes adhered on HyalS stripes while on the Hyal micropatterned surfaces they spread on silanised glass substrate around the structured area, resulting in the exclusion of the topographic pattern. |
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AbstractList | Surface microfabrication techniques were widely utilised for the spatial control of in vitro cell behaviour. A photo-immobilisation procedure was utilised to create micropatterned surfaces: four different stripe patterns (100, 50, 25 and 10 mu m) of hyaluronan (Hyal) and its sulphated derivative (HyalS) on silanised glass substrate were obtained. The morphological analysis showed that the surface topography showed regular stripes of 100, 50, 25 and 10 mu m wide and ranging from 300 nm up to 1 mu m in thickness. They reproduced the exact photo-mask pattern: glass stripes alternating with polysaccharide ones. On the contrary, Hyal microstructures showed just a topographic pattern as the glass stripes appeared to be covered by a thin layer of the macromolecule by TOF-SIMS. Cell adhesion studies demonstrated that melanocytes adhered and oriented within the first 2 h of culture on HyalS microdomains and not on Hyal microstructures where they spread on glass substrate around the patterned area. Double photo-immobilised samples characterised by a 100 mu m stripe pattern of Hyal or HyalS on the top of a continuous layer of the two polysaccharides were also created in order to investigate the effect of the topography on cell behaviour. The obtained results demonstrated that melanocytes adhered on HyalS stripes while on the Hyal micropatterned surfaces they spread on silanised glass substrate around the structured area, resulting in the exclusion of the topographic pattern. Surface microfabrication techniques were widely utilised for the spatial control of in vitro cell behaviour. A photo-immobilisation procedure was utilised to create micropatterned surfaces: four different stripe patterns (100, 50, 25 and 10 μm) of hyaluronan (Hyal) and its sulphated derivative (HyalS) on silanised glass substrate were obtained. The morphological analysis showed that the surface topography showed regular stripes of 100, 50, 25 and 10 μm wide and ranging from 300 nm up to 1 μm in thickness. They reproduced the exact photo-mask pattern: glass stripes alternating with polysaccharide ones. On the contrary, Hyal microstructures showed just a topographic pattern as the glass stripes appeared to be covered by a thin layer of the macromolecule by TOF-SIMS. Cell adhesion studies demonstrated that melanocytes adhered and oriented within the first 2 h of culture on HyalS microdomains and not on Hyal microstructures where they spread on glass substrate around the patterned area. Double photo-immobilised samples characterised by a 100 μm stripe pattern of Hyal or HyalS on the top of a continuous layer of the two polysaccharides were also created in order to investigate the effect of the topography on cell behaviour. The obtained results demonstrated that melanocytes adhered on HyalS stripes while on the Hyal micropatterned surfaces they spread on silanised glass substrate around the structured area, resulting in the exclusion of the topographic pattern. A photo-immobilization procedure was used to create four different stripe micropatterns (100, 50, 25, 10 microns) of hyaluronan (Hyal) and its sulfated derivative (HyalS) on a silanized glass substrate. Morphological analysis showed the stripes were regular and ranged from 300 nm to 1 micron in thickness. Cell adhesion studies showed that melanocytes adhered and oriented within 2 h of culture on HyalS microdomains; but on Hyal microstructures they spread on the glass substrate around the patterned area. Double photo-immobilized samples characterized by a 100-micron striped pattern of Hyal or HyalS on top of a continuous layer of the two polysaccharides were then created, to assess the effect of topography on cell behaviour. The melanocytes adhered to the HyalS stripes, but spread on the glass around the Hyal stripes, excluding the topographic pattern. Surface microfabrication techniques were widely utilised for the spatial control of in vitro cell behaviour. A photo-immobilisation procedure was utilised to create micropatterned surfaces: four different stripe patterns (100, 50, 25 and 10 microm) of hyaluronan (Hyal) and its sulphated derivative (HyalS) on silanised glass substrate were obtained.The morphological analysis showed that the surface topography showed regular stripes of 100, 50, 25 and 10 microm wide and ranging from 300 nm up to 1 microm in thickness. They reproduced the exact photo-mask pattern: glass stripes alternating with polysaccharide ones. On the contrary, Hyal microstructures showed just a topographic pattern as the glass stripes appeared to be covered by a thin layer of the macromolecule by TOF-SIMS. Cell adhesion studies demonstrated that melanocytes adhered and oriented within the first 2h of culture on HyalS microdomains and not on Hyal microstructures where they spread on glass substrate around the patterned area. Double photo-immobilised samples characterised by a 100 microm stripe pattern of Hyal or HyalS on the top of a continuous layer of the two polysaccharides were also created in order to investigate the effect of the topography on cell behaviour. The obtained results demonstrated that melanocytes adhered on HyalS stripes while on the Hyal micropatterned surfaces they spread on silanised glass substrate around the structured area, resulting in the exclusion of the topographic pattern. |
Author | Barbucci, R Lamponi, S Pasqui, D Magnani, A Bryan, S |
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Keywords | Melanocytes Sulphated hyaluronic acid Micropatterned surfaces Cell behaviour |
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Snippet | Surface microfabrication techniques were widely utilised for the spatial control of in vitro cell behaviour. A photo-immobilisation procedure was utilised to... A photo-immobilization procedure was used to create four different stripe micropatterns (100, 50, 25, 10 microns) of hyaluronan (Hyal) and its sulfated... |
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SubjectTerms | Animals Cell Adhesion - drug effects Cell behaviour Cell Culture Techniques - methods Cell Line Cells, Immobilized - drug effects Cells, Immobilized - physiology Cells, Immobilized - ultrastructure Glass Hyaluronic Acid - analogs & derivatives Hyaluronic Acid - chemical synthesis Hyaluronic Acid - pharmacology Melanocytes Melanocytes - drug effects Melanocytes - physiology Melanocytes - ultrastructure Mice Micropatterned surfaces Microscopy, Atomic Force Microscopy, Electron, Scanning Spectrometry, Mass, Secondary Ion Spectroscopy, Fourier Transform Infrared Sulfuric Acid Esters - chemical synthesis Sulfuric Acid Esters - pharmacology Sulphated hyaluronic acid |
Title | The use of hyaluronan and its sulphated derivative patterned with micrometric scale on glass substrate in melanocyte cell behaviour |
URI | https://dx.doi.org/10.1016/S0142-9612(02)00425-8 https://www.ncbi.nlm.nih.gov/pubmed/12504512 https://search.proquest.com/docview/18734047 https://search.proquest.com/docview/28048965 https://search.proquest.com/docview/72938799 |
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