Visual Detection of Ascorbic Acid via Smartphone with Portable Probe Based on Assembled FePO4@GO Peroxidase-like Nanozyme
Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Adequate quantification of AA is required for controlling the intake of AA for health management. In this study, a portable probe was fabricated based on the assembled FePO4@GO nanozyme by homogeneous precipi...
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Published in | Molecules (Basel, Switzerland) Vol. 29; no. 21; p. 5097 |
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Abstract | Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Adequate quantification of AA is required for controlling the intake of AA for health management. In this study, a portable probe was fabricated based on the assembled FePO4@GO nanozyme by homogeneous precipitation. FePO4@GO exhibits excellent peroxidase (POD) activity. As an efficient free radical scavenger, ascorbic acid scavenges 1O2 free radicals produced by hydrogen peroxide catalyzed by peroxidase enzymes and then inhibits the color reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). A good linear relationship between the color of the reaction system and the concentration of AA in the range of 2.5–75 μM was presented, and the detection limit is 1.25 μM. Furthermore, a visual colorimetric detection platform was constructed using a smartphone. The AA content is accurately reflected by intelligent recognition of color changes in the reaction system. It provides an alternative approach for portable and visual rapid detection of Vitamin C (VC) in medicine. Meanwhile, it also provides a reference for the assembly of an intelligent detection platform based on nanozymes. |
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AbstractList | Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Adequate quantification of AA is required for controlling the intake of AA for health management. In this study, a portable probe was fabricated based on the assembled FePO4@GO nanozyme by homogeneous precipitation. FePO4@GO exhibits excellent peroxidase (POD) activity. As an efficient free radical scavenger, ascorbic acid scavenges 1O2 free radicals produced by hydrogen peroxide catalyzed by peroxidase enzymes and then inhibits the color reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). A good linear relationship between the color of the reaction system and the concentration of AA in the range of 2.5–75 μM was presented, and the detection limit is 1.25 μM. Furthermore, a visual colorimetric detection platform was constructed using a smartphone. The AA content is accurately reflected by intelligent recognition of color changes in the reaction system. It provides an alternative approach for portable and visual rapid detection of Vitamin C (VC) in medicine. Meanwhile, it also provides a reference for the assembly of an intelligent detection platform based on nanozymes. Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Adequate quantification of AA is required for controlling the intake of AA for health management. In this study, a portable probe was fabricated based on the assembled FePO4@GO nanozyme by homogeneous precipitation. FePO4@GO exhibits excellent peroxidase (POD) activity. As an efficient free radical scavenger, ascorbic acid scavenges 1O2 free radicals produced by hydrogen peroxide catalyzed by peroxidase enzymes and then inhibits the color reaction of 3,3',5,5'-tetramethylbenzidine (TMB). A good linear relationship between the color of the reaction system and the concentration of AA in the range of 2.5-75 μM was presented, and the detection limit is 1.25 μM. Furthermore, a visual colorimetric detection platform was constructed using a smartphone. The AA content is accurately reflected by intelligent recognition of color changes in the reaction system. It provides an alternative approach for portable and visual rapid detection of Vitamin C (VC) in medicine. Meanwhile, it also provides a reference for the assembly of an intelligent detection platform based on nanozymes.Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Adequate quantification of AA is required for controlling the intake of AA for health management. In this study, a portable probe was fabricated based on the assembled FePO4@GO nanozyme by homogeneous precipitation. FePO4@GO exhibits excellent peroxidase (POD) activity. As an efficient free radical scavenger, ascorbic acid scavenges 1O2 free radicals produced by hydrogen peroxide catalyzed by peroxidase enzymes and then inhibits the color reaction of 3,3',5,5'-tetramethylbenzidine (TMB). A good linear relationship between the color of the reaction system and the concentration of AA in the range of 2.5-75 μM was presented, and the detection limit is 1.25 μM. Furthermore, a visual colorimetric detection platform was constructed using a smartphone. The AA content is accurately reflected by intelligent recognition of color changes in the reaction system. It provides an alternative approach for portable and visual rapid detection of Vitamin C (VC) in medicine. Meanwhile, it also provides a reference for the assembly of an intelligent detection platform based on nanozymes. Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Adequate quantification of AA is required for controlling the intake of AA for health management. In this study, a portable probe was fabricated based on the assembled FePO 4 @GO nanozyme by homogeneous precipitation. FePO 4 @GO exhibits excellent peroxidase (POD) activity. As an efficient free radical scavenger, ascorbic acid scavenges 1 O 2 free radicals produced by hydrogen peroxide catalyzed by peroxidase enzymes and then inhibits the color reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). A good linear relationship between the color of the reaction system and the concentration of AA in the range of 2.5–75 μM was presented, and the detection limit is 1.25 μM. Furthermore, a visual colorimetric detection platform was constructed using a smartphone. The AA content is accurately reflected by intelligent recognition of color changes in the reaction system. It provides an alternative approach for portable and visual rapid detection of Vitamin C (VC) in medicine. Meanwhile, it also provides a reference for the assembly of an intelligent detection platform based on nanozymes. |
Author | Gong, Yan Zhang, Zifang Zhang, Lifang Bai, Junhua |
AuthorAffiliation | 1 School of Chemistry and Materials Science, Shanxi Normal University, Taiyuan 030031, China 3 Collaborative Innovation Center for Shanxi Advanced Permanent Magnetic Materials and Technology, Taiyuan 030031, China 2 School of Life Science, Shanxi Normal University, Taiyuan 030031, China |
AuthorAffiliation_xml | – name: 3 Collaborative Innovation Center for Shanxi Advanced Permanent Magnetic Materials and Technology, Taiyuan 030031, China – name: 1 School of Chemistry and Materials Science, Shanxi Normal University, Taiyuan 030031, China – name: 2 School of Life Science, Shanxi Normal University, Taiyuan 030031, China |
Author_xml | – sequence: 1 givenname: Zifang surname: Zhang fullname: Zhang, Zifang – sequence: 2 givenname: Junhua surname: Bai fullname: Bai, Junhua – sequence: 3 givenname: Yan surname: Gong fullname: Gong, Yan – sequence: 4 givenname: Lifang surname: Zhang fullname: Zhang, Lifang |
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Snippet | Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Adequate quantification of AA is required for controlling the... |
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StartPage | 5097 |
SubjectTerms | ascorbic acid Biocompatibility Composite materials Environmental protection Enzyme kinetics FePO4@GO nanozymes Nanomaterials Nanoparticles Oxidation peroxidase-like nanozymes Smartphones Spectrum analysis visual detection |
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Title | Visual Detection of Ascorbic Acid via Smartphone with Portable Probe Based on Assembled FePO4@GO Peroxidase-like Nanozyme |
URI | https://www.proquest.com/docview/3126012464 https://www.proquest.com/docview/3128745002 https://pubmed.ncbi.nlm.nih.gov/PMC11547973 https://doaj.org/article/a49704323cbd40609fe38eb721745abe |
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