NMR relaxometry and imaging of water absorbed in biodegradable polymer scaffolds
Porous substrates made of poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBHV) were prepared by a particulate leaching method. After removing the salt by extraction in water, proton nuclear magnetic resonance (NMR) relaxometry and imaging were performed on sets of PHBHV substrates immersed in phosphate...
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Published in | Magnetic resonance imaging Vol. 24; no. 1; pp. 89 - 95 |
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Abstract | Porous substrates made of poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBHV) were prepared by a particulate leaching method. After removing the salt by extraction in water, proton nuclear magnetic resonance (NMR) relaxometry and imaging were performed on sets of PHBHV substrates immersed in phosphate-buffered solution during 3 months at different time points. Polarized optical microscopy studies were performed on thin sections, 25 and 5 μm, of the PHBHV samples. The results of NMR relaxometry showed two
1H nuclei populations, well distinguishable on the free induction decay (FID), due to the different decay time constants, a factor of 10
2 apart. Thus, it was possible to separate the two populations, giving separate distributions of
T
1 relaxation times. One population could be associated with water protons in the pores and the other to macromolecular protons. The distributions of
T
1 and
T
2 of the water proton shifted to lower values with increasing immersion time to a constant value after 30 days. The results obtained by NMR imaging showed an initial increase in the apparent porosity, reaching a plateau after 25 days of immersion. This increase is attributed mainly to the absorption of water in the microporosity as supported by the results of the relaxometry measurements and shown by scanning electron microscopy. The average porosity measured by NMR imaging at the plateau, 78±3%, is slightly higher than that determined by optical microscopy, 73±9%, which may be due to the fact that the latter method did not resolve the microporosity. Overall, the results suggest that at early stages after immersing the scaffolds in the aqueous medium, first 30 days approximately, NMR imaging could underestimate the porosity of the substrate. |
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AbstractList | Porous substrates made of poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBHV) were prepared by a particulate leaching method. After removing the salt by extraction in water, proton nuclear magnetic resonance (NMR) relaxometry and imaging were performed on sets of PHBHV substrates immersed in phosphate-buffered solution during 3 months at different time points. Polarized optical microscopy studies were performed on thin sections, 25 and 5 mum, of the PHBHV samples. The results of NMR relaxometry showed two (1)H nuclei populations, well distinguishable on the free induction decay (FID), due to the different decay time constants, a factor of 10(2) apart. Thus, it was possible to separate the two populations, giving separate distributions of T(1) relaxation times. One population could be associated with water protons in the pores and the other to macromolecular protons. The distributions of T(1) and T(2) of the water proton shifted to lower values with increasing immersion time to a constant value after 30 days. The results obtained by NMR imaging showed an initial increase in the apparent porosity, reaching a plateau after 25 days of immersion. This increase is attributed mainly to the absorption of water in the microporosity as supported by the results of the relaxometry measurements and shown by scanning electron microscopy. The average porosity measured by NMR imaging at the plateau, 78+/-3%, is slightly higher than that determined by optical microscopy, 73+/-9%, which may be due to the fact that the latter method did not resolve the microporosity. Overall, the results suggest that at early stages after immersing the scaffolds in the aqueous medium, first 30 days approximately, NMR imaging could underestimate the porosity of the substrate. Porous substrates made of poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBHV) were prepared by a particulate leaching method. After removing the salt by extraction in water, proton nuclear magnetic resonance (NMR) relaxometry and imaging were performed on sets of PHBHV substrates immersed in phosphate-buffered solution during 3 months at different time points. Polarized optical microscopy studies were performed on thin sections, 25 and 5 μm, of the PHBHV samples. The results of NMR relaxometry showed two 1H nuclei populations, well distinguishable on the free induction decay (FID), due to the different decay time constants, a factor of 10 2 apart. Thus, it was possible to separate the two populations, giving separate distributions of T 1 relaxation times. One population could be associated with water protons in the pores and the other to macromolecular protons. The distributions of T 1 and T 2 of the water proton shifted to lower values with increasing immersion time to a constant value after 30 days. The results obtained by NMR imaging showed an initial increase in the apparent porosity, reaching a plateau after 25 days of immersion. This increase is attributed mainly to the absorption of water in the microporosity as supported by the results of the relaxometry measurements and shown by scanning electron microscopy. The average porosity measured by NMR imaging at the plateau, 78±3%, is slightly higher than that determined by optical microscopy, 73±9%, which may be due to the fact that the latter method did not resolve the microporosity. Overall, the results suggest that at early stages after immersing the scaffolds in the aqueous medium, first 30 days approximately, NMR imaging could underestimate the porosity of the substrate. Porous substrates made of poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBHV) were prepared by a particulate leaching method. After removing the salt by extraction in water, proton nuclear magnetic resonance (NMR) relaxometry and imaging were performed on sets of PHBHV substrates immersed in phosphate- buffered solution during 3 months at different time points. Polarized optical microscopy studies were performed on thin sections, 25 and 5 mu m, of the PHBHV samples. The results of NMR relaxometry showed two H nuclei populations, well distinguishable on the free induction decay (FID), due to the different decay time constants, a factor of 10 super(2) apart. Thus, it was possible to separate the two populations, giving separate distributions of T sub(1) relaxation times. One population could be associated with water protons in the pores and the other to macromolecular protons. The distributions of T sub(1) and T sub(2) of the water proton shifted to lower values with increasing immersion time to a constant value after 30 days. The results obtained by NMR imaging showed an initial increase in the apparent porosity, reaching a plateau after 25 days of immersion. This increase is attributed mainly to the absorption of water in the microporosity as supported by the results of the relaxometry measurements and shown by scanning electron microscopy. The average porosity measured by NMR imaging at the plateau, 78+/-3%, is slightly higher than that determined by optical microscopy, 73+/-9%, which may be due to the fact that the latter method did not resolve the microporosity. Overall, the results suggest that at early stages after immersing the scaffolds in the aqueous medium, first 30 days approximately, NMR imaging could underestimate the porosity of the substrate. |
Author | Garrido, Leoncio Fantazzini, Paola Gomez, Santiago Marcos, Marta Garavaglia, Carla Cano, Pilar |
Author_xml | – sequence: 1 givenname: Marta surname: Marcos fullname: Marcos, Marta organization: Department of Physical Chemistry, Institute of Polymer Science and Technology, CSIC, E-28006 Madrid, Spain – sequence: 2 givenname: Pilar surname: Cano fullname: Cano, Pilar organization: Department of Physical Chemistry, Institute of Polymer Science and Technology, CSIC, E-28006 Madrid, Spain – sequence: 3 givenname: Paola surname: Fantazzini fullname: Fantazzini, Paola email: paola.fantazzini@unibo.it organization: Department of Physics, University of Bologna, I-40127 Bologna, Italy – sequence: 4 givenname: Carla surname: Garavaglia fullname: Garavaglia, Carla organization: Department of Physics, University of Bologna, I-40127 Bologna, Italy – sequence: 5 givenname: Santiago surname: Gomez fullname: Gomez, Santiago organization: Department of Pathology, Medical School, University of Cádiz, E-11003 Cádiz, Spain – sequence: 6 givenname: Leoncio surname: Garrido fullname: Garrido, Leoncio organization: Department of Physical Chemistry, Institute of Polymer Science and Technology, CSIC, E-28006 Madrid, Spain |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16410183$$D View this record in MEDLINE/PubMed |
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Keywords | Biodegradation Proton NMR relaxometry Scaffold Poly(3-hydroxybutyrate-3-hydroxyvalerate) MRI |
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doi: 10.1016/0142-9612(92)90099-A contributor: fullname: Knowles – volume: 26 start-page: 5187 year: 2005 ident: 10.1016/j.mri.2005.10.008_bib13 article-title: Tissue engineered cartilage on collagen and PHBV matrices publication-title: Biomaterials doi: 10.1016/j.biomaterials.2005.01.037 contributor: fullname: Köse – volume: 17 start-page: 219 year: 2004 ident: 10.1016/j.mri.2005.10.008_bib20 article-title: Evaluation of cartilage repair tissue after biomaterial implantation in rat patella by using T2 mapping publication-title: Magma doi: 10.1007/s10334-004-0071-7 contributor: fullname: Watrin-Pinzano |
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Snippet | Porous substrates made of poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBHV) were prepared by a particulate leaching method. After removing the salt by... |
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SubjectTerms | Absorption Biodegradation Biodegradation, Environmental Magnetic Resonance Spectroscopy - methods Microscopy, Electron, Scanning MRI Poly(3-hydroxybutyrate-3-hydroxyvalerate) Polyesters - chemistry Porosity Proton NMR relaxometry Scaffold Water |
Title | NMR relaxometry and imaging of water absorbed in biodegradable polymer scaffolds |
URI | https://dx.doi.org/10.1016/j.mri.2005.10.008 https://www.ncbi.nlm.nih.gov/pubmed/16410183 https://search.proquest.com/docview/19440118 |
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