Understanding and modulating apoptosis in industrial cell culture

• Published in: Current Opinion in Biotechnology Vol. 12; no. 2; pp. 175 - 179
• Main Authors: Laken, Haley A, Leonard, Mark W
• Format: Book Review Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2001
• Subjects: Apoptosis; Apoptosis - physiology; Bcl-2; Cell Culture Techniques - methods; Drug Industry; fed-batch; large-scale cell culture; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; Proto-Oncogene Proteins c-bcl-2 - physiology; recombinant protein production; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; serum-free; Bcl-2; Biotechnology; fed-batch; Pharmaceutical Science; large-scale cell culture; serum-free; recombinant protein production; Cell biology; Apoptosis

Currently, the production of therapeutic recombinant proteins relies heavily on the large-scale culture of eukaryotic cells that secrete the protein of interest into the media. It has been recognized that programmed cell death, or apoptosis, may pose a significant hurdle to maximum productivity in s...