Understanding and modulating apoptosis in industrial cell culture

Currently, the production of therapeutic recombinant proteins relies heavily on the large-scale culture of eukaryotic cells that secrete the protein of interest into the media. It has been recognized that programmed cell death, or apoptosis, may pose a significant hurdle to maximum productivity in s...

Full description

Saved in:
Bibliographic Details
Published inCurrent Opinion in Biotechnology Vol. 12; no. 2; pp. 175 - 179
Main Authors Laken, Haley A, Leonard, Mark W
Format Book Review Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.04.2001
Subjects
Apoptosis
Apoptosis - physiology
Bcl-2
Cell Culture Techniques - methods
Drug Industry
fed-batch
large-scale cell culture
Proto-Oncogene Proteins c-bcl-2 - biosynthesis
Proto-Oncogene Proteins c-bcl-2 - physiology
recombinant protein production
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
serum-free
Bcl-2
Biotechnology
fed-batch
Pharmaceutical Science
large-scale cell culture
serum-free
recombinant protein production
Cell biology
Apoptosis
Online AccessGet full text

Cover

More Information
Summary:Currently, the production of therapeutic recombinant proteins relies heavily on the large-scale culture of eukaryotic cells that secrete the protein of interest into the media. It has been recognized that programmed cell death, or apoptosis, may pose a significant hurdle to maximum productivity in such systems. With a greater understanding of the molecular events causing apoptosis, alterations can be made to the cells and culture conditions to prevent apoptosis and enhance volumetric productivity.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
ObjectType-Review-3
content type line 23
ObjectType-Feature-3
ObjectType-Review-1
ISSN:0958-1669
1879-0429
DOI:10.1016/S0958-1669(00)00196-8