Molecular chaperone TRiC governs avian reovirus replication by protecting outer-capsid protein σC and inner core protein σA and non-structural protein σNS from ubiquitin- proteasome degradation

• Published in: Veterinary microbiology Vol. 264; p. 109277
• Main Authors: Huang, Wei-Ru, Li, Jyun-Yi, Liao, Tsai-Ling, Yeh, Chuan-Ming, Wang, Chi-Young, Wen, Hsiao-Wei, Hu, Nien-Jen, Wu, Yi-Ying, Hsu, Chao-Yu, Chang, Yu-Kang, Chang, Ching-Dong, Nielsen, Brent L., Liu, Hung-Jen
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2022; Elsevier BV
• Subjects: Animals; Avian orthoreovirus; Avian reovirus; Capsid protein; Capsid Proteins - metabolism; Chaperonin Containing TCP-1 - metabolism; Chaperonins; Core protein; fluorescent antibody technique; Immunofluorescence; Immunoprecipitation; Non-structural protein Σns; Orthoreovirus, Avian - genetics; Outer and core proteins (σC and σA); poultry; Poultry farming; precipitin tests; proteasome endopeptidase complex; Proteasome Endopeptidase Complex - metabolism; Proteasomes; Proteins; Replication; RNA-Binding Proteins - metabolism; The eukaryotic chaperonin T-complex protein-1 (TCP-1) ring complex (TRiC); Ubiquitin; Ubiquitin - metabolism; Viral Core Proteins - metabolism; viral nonstructural proteins; Viral Proteins - metabolism; Viral Regulatory and Accessory Proteins - metabolism; virus replication; Virus Replication - genetics; Viruses; HIV-1; Non-structural protein Σns; SDS; Hsp; Vero; ARV; MOI; CDK; DMRT; Avian reovirus; DMEM; Outer and core proteins (σC and σA); CCT; HEPES; HSF1A; The eukaryotic chaperonin T-complex protein-1 (TCP-1) ring complex (TRiC); TCP-1; PCR; MDRV; TRiC

•TRiC stabilizes the outer-capsid protein σC, inner core protein σA, and the non-structural protein σNS of ARV.•TriC serves as a chaperone of viral proteins and prevents their degradation via the ubiquitin-proteasome pathway.•Inhibition of TRiC significantly reduced expression levels of σC, σA, and...