INDUCTION OF AP-1 BINDING TO INTRON 1 OF SP-A1 AND SP-A2 IS IMPLICATED IN THE PHORBOL ESTER INHIBITION OF HUMAN SP-A PROMOTER ACTIVITY

• Published in: Experimental lung research Vol. 26; no. 5; pp. 303 - 317
• Main Authors: HOOVER, R. R, PAVLOVIC, J, FLOROS, J
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Informa UK Ltd 2000; Taylor & Francis
• Subjects: Analytical, structural and metabolic biochemistry; Binding Sites; Biological and medical sciences; Chloramphenicol Acetyltransferase Electromobility Shift Assay Phorbol Ester Surfactant Protein A Tpa-INDUCED Complex Transcription; Consensus Sequence; DNA, Neoplasm - chemistry; Down-Regulation - drug effects; Down-Regulation - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression; Glycerolipids, phospholipids; Humans; Introns; Lipids; Other biological molecules; Promoter Regions, Genetic; Proteolipids - metabolism; Pulmonary Surfactant-Associated Protein A; Pulmonary Surfactant-Associated Proteins; Pulmonary Surfactants - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Transcription Factor AP-1 - metabolism; Transcription, Genetic - drug effects; Transfection; Tumor Cells, Cultured; Human; Binding; Promoter; Cell line; Pulmonary surfactant; Transcription; Lung; Glycoprotein; Inhibition; Protein A; Gene expression; Biological activity

A deletional analysis of the SP-A1 promoter in NCI-H441 cells was performed to identify potential cis-acting elements involved in phorbol ester mediated repression of human SP-A transcription. The phorbol ester TPA reduced SP-A1 and SP-A2 promoter activity to approximately 35% to 45% compared to tha...