Lhx1 functions together with Otx2, Foxa2, and Ldb1 to govern anterior mesendoderm, node, and midline development
Gene regulatory networks controlling functional activities of spatially and temporally distinct endodermal cell populations in the early mouse embryo remain ill defined. The T-box transcription factor Eomes, acting downstream from Nodal/Smad signals, directly activates the LIM domain homeobox transc...
Saved in:
Published in | Genes & development Vol. 29; no. 20; pp. 2108 - 2122 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Cold Spring Harbor Laboratory Press
15.10.2015
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Gene regulatory networks controlling functional activities of spatially and temporally distinct endodermal cell populations in the early mouse embryo remain ill defined. The T-box transcription factor Eomes, acting downstream from Nodal/Smad signals, directly activates the LIM domain homeobox transcription factor Lhx1 in the visceral endoderm. Here we demonstrate Smad4/Eomes-dependent Lhx1 expression in the epiblast marks the entire definitive endoderm lineage, the anterior mesendoderm, and midline progenitors. Conditional inactivation of Lhx1 disrupts anterior definitive endoderm development and impedes node and midline morphogenesis in part due to severe disturbances in visceral endoderm displacement. Transcriptional profiling and ChIP-seq (chromatin immunoprecipitation [ChIP] followed by high-throughput sequencing) experiments identified Lhx1 target genes, including numerous anterior definitive endoderm markers and components of the Wnt signaling pathway. Interestingly, Lhx1-binding sites were enriched at enhancers, including the Nodal-proximal epiblast enhancer element and enhancer regions controlling Otx2 and Foxa2 expression. Moreover, in proteomic experiments, we characterized a complex comprised of Lhx1, Otx2, and Foxa2 as well as the chromatin-looping protein Ldb1. These partnerships cooperatively regulate development of the anterior mesendoderm, node, and midline cell populations responsible for establishment of the left-right body axis and head formation. |
---|---|
AbstractList | Gene regulatory networks controlling functional activities of spatially and temporally distinct endodermal cell populations in the early mouse embryo remain ill defined. The T-box transcription factor Eomes, acting downstream from Nodal/Smad signals, directly activates the LIM domain homeobox transcription factor
Lhx1
in the visceral endoderm. Here we demonstrate Smad4/Eomes-dependent
Lhx1
expression in the epiblast marks the entire definitive endoderm lineage, the anterior mesendoderm, and midline progenitors. Conditional inactivation of
Lhx1
disrupts anterior definitive endoderm development and impedes node and midline morphogenesis in part due to severe disturbances in visceral endoderm displacement. Transcriptional profiling and ChIP-seq (chromatin immunoprecipitation [ChIP] followed by high-throughput sequencing) experiments identified Lhx1 target genes, including numerous anterior definitive endoderm markers and components of the Wnt signaling pathway. Interestingly, Lhx1-binding sites were enriched at enhancers, including the
Nodal
-proximal epiblast enhancer element and enhancer regions controlling
Otx2
and
Foxa2
expression. Moreover, in proteomic experiments, we characterized a complex comprised of Lhx1, Otx2, and Foxa2 as well as the chromatin-looping protein Ldb1. These partnerships cooperatively regulate development of the anterior mesendoderm, node, and midline cell populations responsible for establishment of the left–right body axis and head formation. Gene regulatory networks controlling functional activities of spatially and temporally distinct endodermal cell populations in the early mouse embryo remain ill defined. The T-box transcription factor Eomes, acting downstream from Nodal/Smad signals, directly activates the LIM domain homeobox transcription factor Lhx1 in the visceral endoderm. Here we demonstrate Smad4/Eomes-dependent Lhx1 expression in the epiblast marks the entire definitive endoderm lineage, the anterior mesendoderm, and midline progenitors. Conditional inactivation of Lhx1 disrupts anterior definitive endoderm development and impedes node and midline morphogenesis in part due to severe disturbances in visceral endoderm displacement. Transcriptional profiling and ChIP-seq (chromatin immunoprecipitation [ChIP] followed by high-throughput sequencing) experiments identified Lhx1 target genes, including numerous anterior definitive endoderm markers and components of the Wnt signaling pathway. Interestingly, Lhx1-binding sites were enriched at enhancers, including the Nodal-proximal epiblast enhancer element and enhancer regions controlling Otx2 and Foxa2 expression. Moreover, in proteomic experiments, we characterized a complex comprised of Lhx1, Otx2, and Foxa2 as well as the chromatin-looping protein Ldb1. These partnerships cooperatively regulate development of the anterior mesendoderm, node, and midline cell populations responsible for establishment of the left-right body axis and head formation. Costello et al. demonstrate that Smad4/Eomes-dependent Lhx1 expression in the epiblast marks the entire definitive endoderm lineage, the anterior mesendoderm, and midline progenitors. In proteomic experiments, they characterize a complex comprised of Lhx1, Otx2, and Foxa2 as well as the chromatin-looping protein Ldb1. Gene regulatory networks controlling functional activities of spatially and temporally distinct endodermal cell populations in the early mouse embryo remain ill defined. The T-box transcription factor Eomes, acting downstream from Nodal/Smad signals, directly activates the LIM domain homeobox transcription factor Lhx1 in the visceral endoderm. Here we demonstrate Smad4/Eomes-dependent Lhx1 expression in the epiblast marks the entire definitive endoderm lineage, the anterior mesendoderm, and midline progenitors. Conditional inactivation of Lhx1 disrupts anterior definitive endoderm development and impedes node and midline morphogenesis in part due to severe disturbances in visceral endoderm displacement. Transcriptional profiling and ChIP-seq (chromatin immunoprecipitation [ChIP] followed by high-throughput sequencing) experiments identified Lhx1 target genes, including numerous anterior definitive endoderm markers and components of the Wnt signaling pathway. Interestingly, Lhx1-binding sites were enriched at enhancers, including the Nodal -proximal epiblast enhancer element and enhancer regions controlling Otx2 and Foxa2 expression. Moreover, in proteomic experiments, we characterized a complex comprised of Lhx1, Otx2, and Foxa2 as well as the chromatin-looping protein Ldb1. These partnerships cooperatively regulate development of the anterior mesendoderm, node, and midline cell populations responsible for establishment of the left–right body axis and head formation. |
Author | Costello, Ita Nowotschin, Sonja Mould, Arne W Robertson, Elizabeth J Sun, Xin Bikoff, Elizabeth K Hadjantonakis, Anna-Katerina |
AuthorAffiliation | 1 The Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom 2 Developmental Biology Program, Sloan Kettering Institute, New York, New York 10065, USA |
AuthorAffiliation_xml | – name: 2 Developmental Biology Program, Sloan Kettering Institute, New York, New York 10065, USA – name: 1 The Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom |
Author_xml | – sequence: 1 givenname: Ita surname: Costello fullname: Costello, Ita organization: The Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom – sequence: 2 givenname: Sonja surname: Nowotschin fullname: Nowotschin, Sonja organization: Developmental Biology Program, Sloan Kettering Institute, New York, New York 10065, USA – sequence: 3 givenname: Xin surname: Sun fullname: Sun, Xin organization: The Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom – sequence: 4 givenname: Arne W surname: Mould fullname: Mould, Arne W organization: The Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom – sequence: 5 givenname: Anna-Katerina surname: Hadjantonakis fullname: Hadjantonakis, Anna-Katerina organization: Developmental Biology Program, Sloan Kettering Institute, New York, New York 10065, USA – sequence: 6 givenname: Elizabeth K surname: Bikoff fullname: Bikoff, Elizabeth K organization: The Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom – sequence: 7 givenname: Elizabeth J surname: Robertson fullname: Robertson, Elizabeth J organization: The Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26494787$$D View this record in MEDLINE/PubMed |
BookMark | eNpVkc1vGyEQxVHlqHHSXnusOPaQdWFZYLlUqqx8VFopl_SM2GXWptoFF7Dj_Pchcho1p6cZfvNm0LtACx88IPSFkhWlhH7fGLuqRaukKjX_gJaUN6rijZQLtCStIpViQp2ji5T-EEIEEeIjOq9FoxrZyiXaddsjxePeD9kFn3AOG8hbiPjR5S2-z8f6Ct-EoylivMWd7Wlh8CYcIPrSyhBdiHiGBN4GC3G-wr7oCZ-dnZwHbOEAU9jN4PMndDaaKcHnV71Ev2-uH9Z3VXd_-2v9s6uGpma5Uk09CDpyJZii3EgmBjISaKwcpWkpYdAPrAUueM8VU5YYrmpOgI_ECttbdol-nHx3-34GO5TV0Ux6F91s4pMOxun3L95tdfmWbgSVSopi8O3VIIa_e0hZzy4NME3GQ9gnTaUUQrCa0YKuTugQQ0oRxrc1lOiXmHSJSZ9iKjUvA1__P-4N_5cLewYRnZD4 |
CitedBy_id | crossref_primary_10_1093_hmg_ddab195 crossref_primary_10_15252_emmm_202012574 crossref_primary_10_1016_j_ydbio_2023_07_002 crossref_primary_10_1038_s41467_024_46773_z crossref_primary_10_1093_nar_gkx433 crossref_primary_10_1007_s11427_016_0285_3 crossref_primary_10_1101_gr_277960_123 crossref_primary_10_1016_j_celrep_2022_111219 crossref_primary_10_3389_fgene_2024_1401315 crossref_primary_10_1016_j_isci_2022_105891 crossref_primary_10_1002_wsbm_1436 crossref_primary_10_1126_sciadv_adl4239 crossref_primary_10_1038_s41586_023_06914_8 crossref_primary_10_1016_j_stemcr_2024_05_010 crossref_primary_10_1007_s00239_021_10012_6 crossref_primary_10_1371_journal_pbio_3001924 crossref_primary_10_1155_2018_3610603 crossref_primary_10_1371_journal_pbio_3001200 crossref_primary_10_1007_s12020_021_02682_5 crossref_primary_10_1242_jcs_261151 crossref_primary_10_1091_mbc_E21_02_0089 crossref_primary_10_1242_bio_059307 crossref_primary_10_3389_fgene_2021_760450 crossref_primary_10_1126_sciadv_aax9852 crossref_primary_10_1523_ENEURO_0356_18_2019 crossref_primary_10_1111_cpr_13298 crossref_primary_10_7554_eLife_33333 crossref_primary_10_1242_dev_149724 crossref_primary_10_1002_jcp_27736 crossref_primary_10_1002_dvdy_24392 crossref_primary_10_1038_s41556_022_00923_x crossref_primary_10_1210_er_2016_1101 crossref_primary_10_3389_fcell_2021_777652 crossref_primary_10_1093_bioinformatics_btaa690 crossref_primary_10_1038_s41388_018_0413_y crossref_primary_10_1016_j_stemcr_2021_03_017 crossref_primary_10_1111_dgd_12609 crossref_primary_10_1038_ncomms14428 crossref_primary_10_1002_clt2_12131 crossref_primary_10_1016_j_celrep_2017_05_072 crossref_primary_10_3390_cells9020509 crossref_primary_10_3389_fonc_2023_1082441 crossref_primary_10_1002_dvg_23246 crossref_primary_10_1186_s12967_024_05125_7 crossref_primary_10_1038_s42003_023_04810_5 crossref_primary_10_18632_oncotarget_21772 crossref_primary_10_1242_dev_133652 crossref_primary_10_1007_s00427_024_00718_0 crossref_primary_10_1016_j_cell_2021_04_004 crossref_primary_10_1016_j_isci_2023_108205 crossref_primary_10_1038_s41598_018_34038_x crossref_primary_10_1073_pnas_2311946120 crossref_primary_10_1016_j_reprotox_2019_10_001 crossref_primary_10_3892_mmr_2018_9201 crossref_primary_10_1016_j_csbj_2019_04_003 crossref_primary_10_1038_s41467_021_23653_4 crossref_primary_10_1242_dev_202545 crossref_primary_10_1242_dev_147322 crossref_primary_10_1016_j_stem_2016_10_002 crossref_primary_10_1002_pd_4926 crossref_primary_10_1016_j_bbagrm_2019_04_003 crossref_primary_10_1002_dvdy_217 crossref_primary_10_1371_journal_pone_0186150 crossref_primary_10_1093_nar_gkac542 crossref_primary_10_1016_j_isci_2023_108051 crossref_primary_10_1016_j_ydbio_2016_08_008 crossref_primary_10_1038_s41598_023_43662_1 |
Cites_doi | 10.1038/384270a0 10.1016/S0168-9525(99)01883-1 10.1101/gad.211912.112 10.1186/1471-213X-1-4 10.1016/j.cell.2012.03.051 10.1016/S0092-8674(00)81537-8 10.1073/pnas.0605209103 10.1038/ncb2304 10.1002/dvdy.21822 10.1002/dvdy.22366 10.1186/gb-2008-9-9-r137 10.1002/dvdy.21598 10.1016/j.ydbio.2010.07.029 10.1101/gad.13.12.1589 10.1242/dev.126.22.4925 10.1101/gad.607311 10.1016/j.cell.2008.05.024 10.1101/gad.13.12.1575 10.1101/gad.1100503 10.1038/nbt.1630 10.1016/j.devcel.2006.07.005 10.1242/dev.00176 10.1242/dev.01248 10.1002/dvdy.22417 10.1093/bioinformatics/btq081 10.1016/j.celrep.2014.05.037 10.1101/gr.111120.110 10.1242/dev.000885 10.1002/1526-968X(200005)27:1<12::AID-GENE30>3.0.CO;2-X 10.1242/dev.01219 10.1101/gr.1362303 10.1371/journal.pgen.1002770 10.1038/374425a0 10.1016/S1534-5807(01)00041-7 10.1093/nar/gkh465 10.1006/dbio.1994.1018 10.1016/j.ydbio.2011.10.002 10.1101/gad.215152.113 10.1242/dev.01220 10.1371/journal.pone.0081854 10.1038/nrm2618 10.1093/nar/gkn382 10.1016/j.gde.2013.03.001 10.1242/dev.118.1.47 10.1101/gad.1528507 10.4161/cc.11.1.18700 10.1016/j.devcel.2008.07.017 10.1242/dev.121.10.3291 10.1242/dev.01844 10.1038/ncb3070 10.1038/5007 10.1371/journal.pbio.1001276 10.1242/dev.00225 10.1101/gad.1084403 10.1006/bbrc.1999.1872 10.1242/dev.028415 10.1038/381155a0 10.1101/gad.199828.112 10.1242/dev.120907 10.1016/j.ydbio.2005.12.053 10.1002/dvdy.20843 10.1242/dev.014357 10.1002/1526-968X(200011/12)28:3/4<106::AID-GENE30>3.0.CO;2-T 10.1002/dvdy.21313 10.1046/j.1365-2443.1996.04004.x 10.1093/jmcb/mjs037 10.1242/dev.122.1.243 10.1073/pnas.1105852108 10.1016/j.stem.2014.04.003 10.1101/gad.239749.114 10.1016/j.stem.2008.07.021 10.1186/1471-2164-9-511 10.1073/pnas.0703738104 10.1016/S0925-4773(03)00099-6 10.1002/pmic.201000800 10.1016/j.tig.2013.10.001 10.1016/0092-8674(94)90522-3 10.1016/j.stem.2015.08.007 10.1242/dev.114900 10.1038/383407a0 10.1242/dev.053801 10.1242/dev.126.20.4499 10.1006/dbio.1999.9256 10.1038/nrg2084 10.1016/j.devcel.2007.10.016 |
ContentType | Journal Article |
Copyright | 2015 Costello et al.; Published by Cold Spring Harbor Laboratory Press. 2015 |
Copyright_xml | – notice: 2015 Costello et al.; Published by Cold Spring Harbor Laboratory Press. – notice: 2015 |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7TM 8FD FR3 P64 RC3 5PM |
DOI | 10.1101/gad.268979.115 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Nucleic Acids Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts Genetics Abstracts PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Genetics Abstracts Engineering Research Database Technology Research Database Nucleic Acids Abstracts Biotechnology and BioEngineering Abstracts |
DatabaseTitleList | CrossRef Genetics Abstracts MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
DocumentTitleAlternate | Costello et al |
EISSN | 1549-5477 |
EndPage | 2122 |
ExternalDocumentID | 10_1101_gad_268979_115 26494787 |
Genre | Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
GrantInformation_xml | – fundername: NICHD NIH HHS grantid: R01-HD052115 – fundername: NICHD NIH HHS grantid: R01 HD052115 – fundername: NIDDK NIH HHS grantid: R01 DK084391 – fundername: Wellcome Trust grantid: 091911 – fundername: NIDDK NIH HHS grantid: R01-DK084391 – fundername: Wellcome Trust grantid: 102811 – fundername: Medical Research Council grantid: G0900747 91070 – fundername: NCI NIH HHS grantid: P30 CA008748 – fundername: Wellcome Trust grantid: 090532/Z/09/Z – fundername: NCI NIH HHS grantid: P30-CA008748 – fundername: Medical Research Council Hub grantid: G0900747 91070 – fundername: National Institutes of Health grantid: R01-DK084391; R01-HD052115; P30-CA008748 |
GroupedDBID | --- -DZ -~X .55 18M 29H 2WC 39C 4.4 53G 5RE 5VS 85S ABCQX ABDIX ACGFO ACNCT ADBBV ADIYS AECCQ AENEX AFFNX AFOSN ALMA_UNASSIGNED_HOLDINGS BAWUL BTFSW CGR CS3 CUY CVF DIK DU5 E3Z EBS ECM EIF EJD F5P FRP GX1 H13 HYE H~9 IH2 KQ8 L7B MV1 N9A NPM OK1 P2P R.V RCX RHF RHI RPM SJN TAE TN5 TR2 UHB VQA W8F WH7 WOQ X7M XJT XSW YBU YHG YKV YSK AAYXX CITATION 7TM 8FD AETEA FR3 P64 RC3 5PM |
ID | FETCH-LOGICAL-c423t-942c61f5963915a736c0f0e4d7f7a8103ebc38e565b5939d0a59250e5f0d6dbd3 |
IEDL.DBID | RPM |
ISSN | 0890-9369 |
IngestDate | Tue Sep 17 21:29:15 EDT 2024 Sat Aug 17 00:00:57 EDT 2024 Thu Sep 26 16:49:03 EDT 2024 Sat Sep 28 08:20:44 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 20 |
Keywords | mesendoderm Lhx1 node definitive endoderm Ldb1 midline |
Language | English |
License | 2015 Costello et al.; Published by Cold Spring Harbor Laboratory Press. This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c423t-942c61f5963915a736c0f0e4d7f7a8103ebc38e565b5939d0a59250e5f0d6dbd3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617976/ |
PMID | 26494787 |
PQID | 1776663231 |
PQPubID | 23462 |
PageCount | 15 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_4617976 proquest_miscellaneous_1776663231 crossref_primary_10_1101_gad_268979_115 pubmed_primary_26494787 |
PublicationCentury | 2000 |
PublicationDate | 2015-Oct-15 2015-10-15 20151015 |
PublicationDateYYYYMMDD | 2015-10-15 |
PublicationDate_xml | – month: 10 year: 2015 text: 2015-Oct-15 day: 15 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Genes & development |
PublicationTitleAlternate | Genes Dev |
PublicationYear | 2015 |
Publisher | Cold Spring Harbor Laboratory Press |
Publisher_xml | – name: Cold Spring Harbor Laboratory Press |
References | 2021111619411448000_29.20.2108.35 2021111619411448000_29.20.2108.79 2021111619411448000_29.20.2108.34 2021111619411448000_29.20.2108.78 2021111619411448000_29.20.2108.33 2021111619411448000_29.20.2108.77 2021111619411448000_29.20.2108.32 2021111619411448000_29.20.2108.76 2021111619411448000_29.20.2108.39 2021111619411448000_29.20.2108.38 2021111619411448000_29.20.2108.37 2021111619411448000_29.20.2108.36 2021111619411448000_29.20.2108.82 2021111619411448000_29.20.2108.81 2021111619411448000_29.20.2108.80 2021111619411448000_29.20.2108.42 2021111619411448000_29.20.2108.86 2021111619411448000_29.20.2108.41 2021111619411448000_29.20.2108.85 2021111619411448000_29.20.2108.40 2021111619411448000_29.20.2108.84 2021111619411448000_29.20.2108.83 2021111619411448000_29.20.2108.24 2021111619411448000_29.20.2108.68 2021111619411448000_29.20.2108.23 (2021111619411448000_29.20.2108.59) 1999; 126 2021111619411448000_29.20.2108.67 2021111619411448000_29.20.2108.22 2021111619411448000_29.20.2108.66 2021111619411448000_29.20.2108.21 2021111619411448000_29.20.2108.28 2021111619411448000_29.20.2108.27 2021111619411448000_29.20.2108.26 2021111619411448000_29.20.2108.25 2021111619411448000_29.20.2108.69 2021111619411448000_29.20.2108.29 (2021111619411448000_29.20.2108.57) 1995; 121 (2021111619411448000_29.20.2108.70) 2002; 62 2021111619411448000_29.20.2108.71 (2021111619411448000_29.20.2108.62) 1993; 118 2021111619411448000_29.20.2108.31 2021111619411448000_29.20.2108.75 2021111619411448000_29.20.2108.30 2021111619411448000_29.20.2108.74 2021111619411448000_29.20.2108.73 2021111619411448000_29.20.2108.72 2021111619411448000_29.20.2108.13 2021111619411448000_29.20.2108.12 2021111619411448000_29.20.2108.56 2021111619411448000_29.20.2108.11 2021111619411448000_29.20.2108.55 2021111619411448000_29.20.2108.10 2021111619411448000_29.20.2108.54 2021111619411448000_29.20.2108.17 2021111619411448000_29.20.2108.16 2021111619411448000_29.20.2108.15 2021111619411448000_29.20.2108.14 2021111619411448000_29.20.2108.58 2021111619411448000_29.20.2108.19 2021111619411448000_29.20.2108.18 2021111619411448000_29.20.2108.60 (2021111619411448000_29.20.2108.4) 1996; 122 2021111619411448000_29.20.2108.20 2021111619411448000_29.20.2108.64 2021111619411448000_29.20.2108.63 2021111619411448000_29.20.2108.61 2021111619411448000_29.20.2108.46 2021111619411448000_29.20.2108.45 2021111619411448000_29.20.2108.44 2021111619411448000_29.20.2108.43 2021111619411448000_29.20.2108.49 2021111619411448000_29.20.2108.48 2021111619411448000_29.20.2108.47 (2021111619411448000_29.20.2108.65) 1999; 126 2021111619411448000_29.20.2108.3 2021111619411448000_29.20.2108.1 2021111619411448000_29.20.2108.2 2021111619411448000_29.20.2108.7 2021111619411448000_29.20.2108.8 2021111619411448000_29.20.2108.5 2021111619411448000_29.20.2108.6 2021111619411448000_29.20.2108.9 2021111619411448000_29.20.2108.53 2021111619411448000_29.20.2108.52 2021111619411448000_29.20.2108.51 2021111619411448000_29.20.2108.50 |
References_xml | – ident: 2021111619411448000_29.20.2108.2 doi: 10.1038/384270a0 – ident: 2021111619411448000_29.20.2108.32 doi: 10.1016/S0168-9525(99)01883-1 – ident: 2021111619411448000_29.20.2108.15 doi: 10.1101/gad.211912.112 – ident: 2021111619411448000_29.20.2108.69 doi: 10.1186/1471-213X-1-4 – ident: 2021111619411448000_29.20.2108.23 doi: 10.1016/j.cell.2012.03.051 – ident: 2021111619411448000_29.20.2108.31 doi: 10.1016/S0092-8674(00)81537-8 – ident: 2021111619411448000_29.20.2108.24 doi: 10.1073/pnas.0605209103 – ident: 2021111619411448000_29.20.2108.22 doi: 10.1038/ncb2304 – ident: 2021111619411448000_29.20.2108.33 doi: 10.1002/dvdy.21822 – ident: 2021111619411448000_29.20.2108.43 doi: 10.1002/dvdy.22366 – ident: 2021111619411448000_29.20.2108.86 doi: 10.1186/gb-2008-9-9-r137 – ident: 2021111619411448000_29.20.2108.40 doi: 10.1002/dvdy.21598 – ident: 2021111619411448000_29.20.2108.42 doi: 10.1016/j.ydbio.2010.07.029 – ident: 2021111619411448000_29.20.2108.1 doi: 10.1101/gad.13.12.1589 – volume: 126 start-page: 4925 year: 1999 ident: 2021111619411448000_29.20.2108.65 article-title: Lim1 is required in both primitive streak-derived tissues and visceral endoderm for head formation in the mouse publication-title: Development doi: 10.1242/dev.126.22.4925 – ident: 2021111619411448000_29.20.2108.75 doi: 10.1101/gad.607311 – ident: 2021111619411448000_29.20.2108.10 doi: 10.1016/j.cell.2008.05.024 – ident: 2021111619411448000_29.20.2108.55 doi: 10.1101/gad.13.12.1575 – ident: 2021111619411448000_29.20.2108.78 doi: 10.1101/gad.1100503 – ident: 2021111619411448000_29.20.2108.47 doi: 10.1038/nbt.1630 – ident: 2021111619411448000_29.20.2108.9 doi: 10.1016/j.devcel.2006.07.005 – ident: 2021111619411448000_29.20.2108.60 doi: 10.1242/dev.00176 – ident: 2021111619411448000_29.20.2108.19 doi: 10.1242/dev.01248 – ident: 2021111619411448000_29.20.2108.74 doi: 10.1002/dvdy.22417 – ident: 2021111619411448000_29.20.2108.76 doi: 10.1093/bioinformatics/btq081 – ident: 2021111619411448000_29.20.2108.85 doi: 10.1016/j.celrep.2014.05.037 – ident: 2021111619411448000_29.20.2108.45 doi: 10.1101/gr.111120.110 – ident: 2021111619411448000_29.20.2108.41 doi: 10.1242/dev.000885 – ident: 2021111619411448000_29.20.2108.16 doi: 10.1002/1526-968X(200005)27:1<12::AID-GENE30>3.0.CO;2-X – ident: 2021111619411448000_29.20.2108.37 doi: 10.1242/dev.01219 – ident: 2021111619411448000_29.20.2108.68 doi: 10.1101/gr.1362303 – ident: 2021111619411448000_29.20.2108.11 doi: 10.1371/journal.pgen.1002770 – ident: 2021111619411448000_29.20.2108.64 doi: 10.1038/374425a0 – ident: 2021111619411448000_29.20.2108.51 doi: 10.1016/S1534-5807(01)00041-7 – ident: 2021111619411448000_29.20.2108.58 doi: 10.1093/nar/gkh465 – ident: 2021111619411448000_29.20.2108.8 doi: 10.1006/dbio.1994.1018 – ident: 2021111619411448000_29.20.2108.73 doi: 10.1016/j.ydbio.2011.10.002 – ident: 2021111619411448000_29.20.2108.56 doi: 10.1101/gad.215152.113 – ident: 2021111619411448000_29.20.2108.36 doi: 10.1242/dev.01220 – ident: 2021111619411448000_29.20.2108.20 doi: 10.1371/journal.pone.0081854 – ident: 2021111619411448000_29.20.2108.6 doi: 10.1038/nrm2618 – ident: 2021111619411448000_29.20.2108.81 doi: 10.1093/nar/gkn382 – ident: 2021111619411448000_29.20.2108.5 doi: 10.1016/j.gde.2013.03.001 – volume: 118 start-page: 47 year: 1993 ident: 2021111619411448000_29.20.2108.62 article-title: Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo publication-title: Development doi: 10.1242/dev.118.1.47 – ident: 2021111619411448000_29.20.2108.82 doi: 10.1101/gad.1528507 – ident: 2021111619411448000_29.20.2108.26 doi: 10.4161/cc.11.1.18700 – ident: 2021111619411448000_29.20.2108.39 doi: 10.1016/j.devcel.2008.07.017 – volume: 121 start-page: 3291 year: 1995 ident: 2021111619411448000_29.20.2108.57 article-title: Disruption of the mouse RBP-Jκ gene results in early embryonic death publication-title: Development doi: 10.1242/dev.121.10.3291 – ident: 2021111619411448000_29.20.2108.54 doi: 10.1242/dev.01844 – ident: 2021111619411448000_29.20.2108.80 doi: 10.1038/ncb3070 – ident: 2021111619411448000_29.20.2108.67 doi: 10.1038/5007 – ident: 2021111619411448000_29.20.2108.79 doi: 10.1371/journal.pbio.1001276 – ident: 2021111619411448000_29.20.2108.52 doi: 10.1242/dev.00225 – ident: 2021111619411448000_29.20.2108.61 doi: 10.1101/gad.1084403 – ident: 2021111619411448000_29.20.2108.53 doi: 10.1006/bbrc.1999.1872 – ident: 2021111619411448000_29.20.2108.13 doi: 10.1242/dev.028415 – ident: 2021111619411448000_29.20.2108.21 doi: 10.1038/381155a0 – ident: 2021111619411448000_29.20.2108.50 doi: 10.1101/gad.199828.112 – ident: 2021111619411448000_29.20.2108.27 doi: 10.1242/dev.120907 – ident: 2021111619411448000_29.20.2108.18 doi: 10.1016/j.ydbio.2005.12.053 – ident: 2021111619411448000_29.20.2108.38 doi: 10.1002/dvdy.20843 – ident: 2021111619411448000_29.20.2108.7 doi: 10.1242/dev.014357 – ident: 2021111619411448000_29.20.2108.25 doi: 10.1002/1526-968X(200011/12)28:3/4<106::AID-GENE30>3.0.CO;2-T – ident: 2021111619411448000_29.20.2108.46 doi: 10.1002/dvdy.21313 – ident: 2021111619411448000_29.20.2108.63 doi: 10.1046/j.1365-2443.1996.04004.x – ident: 2021111619411448000_29.20.2108.83 doi: 10.1093/jmcb/mjs037 – volume: 122 start-page: 243 year: 1996 ident: 2021111619411448000_29.20.2108.4 article-title: A targeted mouse Otx2 mutation leads to severe defects in gastrulation and formation of axial mesoderm and to deletion of rostral brain publication-title: Development doi: 10.1242/dev.122.1.243 – ident: 2021111619411448000_29.20.2108.29 doi: 10.1073/pnas.1105852108 – ident: 2021111619411448000_29.20.2108.12 doi: 10.1016/j.stem.2014.04.003 – ident: 2021111619411448000_29.20.2108.35 doi: 10.1101/gad.239749.114 – ident: 2021111619411448000_29.20.2108.48 doi: 10.1016/j.stem.2008.07.021 – ident: 2021111619411448000_29.20.2108.72 doi: 10.1186/1471-2164-9-511 – ident: 2021111619411448000_29.20.2108.28 doi: 10.1073/pnas.0703738104 – volume: 62 start-page: 5651 year: 2002 ident: 2021111619411448000_29.20.2108.70 article-title: Isolation of a novel human gene, APCDD1, as a direct target of the β-catenin/T-cell factor 4 complex with probable involvement in colorectal carcinogenesis publication-title: Cancer Res – ident: 2021111619411448000_29.20.2108.30 doi: 10.1016/S0925-4773(03)00099-6 – ident: 2021111619411448000_29.20.2108.77 doi: 10.1002/pmic.201000800 – ident: 2021111619411448000_29.20.2108.44 doi: 10.1016/j.tig.2013.10.001 – ident: 2021111619411448000_29.20.2108.3 doi: 10.1016/0092-8674(94)90522-3 – ident: 2021111619411448000_29.20.2108.14 doi: 10.1016/j.stem.2015.08.007 – ident: 2021111619411448000_29.20.2108.34 doi: 10.1242/dev.114900 – ident: 2021111619411448000_29.20.2108.17 doi: 10.1038/383407a0 – ident: 2021111619411448000_29.20.2108.49 doi: 10.1242/dev.053801 – volume: 126 start-page: 4499 year: 1999 ident: 2021111619411448000_29.20.2108.59 article-title: HNF3β and Lim1 interact in the visceral endoderm to regulate primitive streak formation and anterior–posterior polarity in the mouse embryo publication-title: Development doi: 10.1242/dev.126.20.4499 – ident: 2021111619411448000_29.20.2108.66 doi: 10.1006/dbio.1999.9256 – ident: 2021111619411448000_29.20.2108.71 doi: 10.1038/nrg2084 – ident: 2021111619411448000_29.20.2108.84 doi: 10.1016/j.devcel.2007.10.016 |
SSID | ssj0006066 |
Score | 2.4883926 |
Snippet | Gene regulatory networks controlling functional activities of spatially and temporally distinct endodermal cell populations in the early mouse embryo remain... Costello et al. demonstrate that Smad4/Eomes-dependent Lhx1 expression in the epiblast marks the entire definitive endoderm lineage, the anterior mesendoderm,... |
SourceID | pubmedcentral proquest crossref pubmed |
SourceType | Open Access Repository Aggregation Database Index Database |
StartPage | 2108 |
SubjectTerms | DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Embryo, Mammalian Embryonic Development - genetics Enhancer Elements, Genetic - physiology Gene Deletion Gene Expression Profiling Gene Expression Regulation, Developmental Germ Layers - embryology Germ Layers - metabolism Hepatocyte Nuclear Factor 3-beta - metabolism LIM Domain Proteins - metabolism LIM-Homeodomain Proteins - genetics LIM-Homeodomain Proteins - metabolism Multiprotein Complexes - genetics Multiprotein Complexes - metabolism Otx Transcription Factors - metabolism Protein Binding Research Paper Transcription Factors - genetics Transcription Factors - metabolism Wnt Signaling Pathway |
Title | Lhx1 functions together with Otx2, Foxa2, and Ldb1 to govern anterior mesendoderm, node, and midline development |
URI | https://www.ncbi.nlm.nih.gov/pubmed/26494787 https://search.proquest.com/docview/1776663231 https://pubmed.ncbi.nlm.nih.gov/PMC4617976 |
Volume | 29 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3dS-QwEB9cQfBF7s6v9TyJIPhid5OmaZrHQ07kzk9Q8K2kSbou2O7iVfD-eydpq7f65lMInZYyM838ks7MD-AAF30thRWR0FkSJalJo4ynZWSZNAU1QljuC5zPL9LT2-T3nbhbAtHXwoSkfVNMR_VDNaqn9yG3cl6ZcZ8nNr46P04w7GIYHQ9gIDnvt-jd8usReYCOikaera7r1IiuN55oO4rTTEmFc89Zg2hA-e40i0HpA9J8nzD5XwQ6-QJrHXQkP9tX_ApLrv4GKy2Z5L91mJ_dPzPi41RwJdLMJqGYl_ijVnLZPMdHBGONxkHXlpzZgqEMmQSyXRIUPJ09ksqXI1nPkFYdkRrHVryaWg9IiX1LMtqA25NfN8enUcenEBkETU2kktikrBT4zSkmtOSpoSV1iZWl1Bmj3BWGZw4hXiEUV5ZqoRAhOVFSTztl-SYs17PabQNJY1rwuHQImFRiUI5zR6njkooikTEfwmGv0Hzets3Iw3aDshytkLdWwLkYwn6v7xw92_-u0LWbPf3NmZS4t-IIQIew1er_9Vm94YYgFyzzKuC7Zi9eQWcK3bM759n59J3fYRVRU-jfysQuLDePT-4HIpOm2IPBn-tsL_jjC2pE4uI |
link.rule.ids | 230,315,733,786,790,891,27955,27956,53825,53827 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB6VIgQXyquwlIeRkLg0WTuO4_hYVVQL7BYOLeotcmxnu4JkVyUrFX59x05S2HKCk2V5EiX6Zjyfk3kAvMVNX0thRSR0nkZpZrIo51kVWSZNSY0QlvsE59lxNjlNP56Jsy0QQy5MCNo35SJuvtdxszgPsZWr2oyHOLHxl9lhim4X3ej4FtxGe03EcEjvN2DPyQN5VDTy_er6Wo2ofOO5tnGS5UoqnPuuNcgHlK9Ps-mW_uKaN0Mm__BBRzvwdXj6LvTkW7xuy9j8ulHY8Z9f7wHc71kpOeiWH8KWax7Bna5P5c_HsJqeXzLiXWDQUtIu5yFPmPivuORze5nsE3RjGgfdWDK1JUMZMg99fEnAbrG8ILXPdLK--Vq9TxocO_F6YT3XJfZ3_NITOD16f3I4ifpWDZFBPtZGKk1MxiqB5qyY0JJnhlbUpVZWUueMclcanjtkj6VQXFmqhULy5URFfUcry3dhu1k27hmQLKElTyqHXEylBuU4d5Q6LqkoU5nwEbwbkCpWXUWOIpxkKCsQ3qKDF-diBG8GIAs0Gv8nRDduuf5RMCnx2MaR247gaQfs9b0GjRiB3ID8WsAX5N5cQSBDYe4euOf_feVruDs5mU2L6YfjT3twD8lZKBPLxAvYbi_W7iUSoLZ8FdT9CnOiA_o |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BEYgLb-jyNBISlyZx4jiOj6iwKrAtPVCp4hL5le2KJrsqWanw6xk7SemWW0-WlUkk6xt7Psfj-QDe4aKvBLc84qrMo7wwRVSyoo5sKoymhnPL_AXn_YNi7yj_csyPL0l9haR9oxdxe9rE7eIk5FauGpOMeWLJ4f5ujmEXw2iysnVyE27hnM3EuFEfFmHPywOBlDTymnVDvUZ0wGSubJwVpRQS-165BjmB9DVqNkPTf3zzatrkpTg0vQ8_xhH06Sc_43WnY_PnSnHHaw3xAdwb2Cn50Js8hBuufQS3e73K349hNTs5T4kPhcFbSbech_vCxP_NJd-682yHYDhT2KjWkpnVKdqQedDzJQHDxfKMNP7Gk_UibM0OabHtzZuF9ZyX2H95TE_gaPrp--5eNEg2RAZ5WRfJPDNFWnOc1jLlSrDC0Jq63IpaqDKlzGnDSocsUnPJpKWKSyRhjtfUK1tZ9hS22mXrtoEUGdUsqx1yMpkbtGPMUeqYoFznImMTeD-iVa36yhxV2NHQtEKIqx5i7PMJvB3BrHDy-BMR1brl-leVCoHbN4YcdwLPenAvvjV6xQTEBuwXBr4w9-YTBDMU6B7Ae37tN9_AncOP02r2-eDrC7iLHC1Ui035S9jqztbuFfKgTr8OHv8XqZcGeg |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Lhx1+functions+together+with+Otx2%2C+Foxa2%2C+and+Ldb1+to+govern+anterior+mesendoderm%2C+node%2C+and+midline+development&rft.jtitle=Genes+%26+development&rft.au=Costello%2C+Ita&rft.au=Nowotschin%2C+Sonja&rft.au=Sun%2C+Xin&rft.au=Mould%2C+Arne+W&rft.date=2015-10-15&rft.issn=0890-9369&rft.eissn=1549-5477&rft.volume=29&rft.issue=20&rft.spage=2108&rft.epage=2122&rft_id=info:doi/10.1101%2Fgad.268979.115&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0890-9369&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0890-9369&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0890-9369&client=summon |