A comparative study of N.C.A. Fluorine-18 labeling of proteins via acylation and photochemical conjugation

• Published in: Nuclear medicine and biology Vol. 23; no. 3; pp. 365 - 372
• Main Authors: Wester, Hans-Jürgen, Hamacher, Kurt, Stöcklin, Gerhard
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.1996
• Subjects: Acylation; Animals; Antibody labeling; Avidin; Azides; Benzoates; Conjugation; Fluorine Radioisotopes - pharmacokinetics; Fluorine-18; Humans; Immunoglobulin G; In vivo stability; Indicators and Reagents; Isotope Labeling - methods; Mice; Mice, Inbred Strains; Photochemistry; Proteins; Proteins - pharmacokinetics; Serum Albumin; Succinimides; Tissue Distribution; Transferrin; Proteins; Fluorine-18; Conjugation; In vivo stability; Antibody labeling

Three methods for 18F-labeling of proteins were evaluated with respect to conjugation yields, suitability for remote-controlled routine synthesis, and in vivo stability of the conjugates— i.e., photochemical conjugation (PCC) using 4-azidophenacyl-[ 18F]fluoride ([ 18F]APF) as well as classical conj...