Lsm Proteins Promote Regeneration of Pre-mRNA Splicing Activity
Lsm proteins are ubiquitous, multifunctional proteins that affect the processing of most RNAs in eukaryotic cells [1–11], but their function is unknown. A complex of seven Lsm proteins, Lsm2-8, associates with the U6 small nuclear RNA (snRNA) that is a component of spliceosome complexes in which pre...
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| Published in | Current biology Vol. 14; no. 16; pp. 1487 - 1491 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
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England
Elsevier Inc
24.08.2004
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| Abstract | Lsm proteins are ubiquitous, multifunctional proteins that affect the processing of most RNAs in eukaryotic cells [1–11], but their function is unknown. A complex of seven Lsm proteins, Lsm2-8, associates with the U6 small nuclear RNA (snRNA) that is a component of spliceosome complexes in which pre-mRNA splicing occurs. Spliceosomes contain five snRNAs, U1, U2, U4, U5, and U6, that are packaged as ribonucleoprotein particles (snRNPs) [12, 13]. U4 and U6 snRNAs contain extensive sequence complementarity and interact to form U4/U6 di-snRNPs. U4/U6 di-snRNPs associate with U5 snRNPs to form U4/U6.U5 tri-snRNPs prior to spliceosome assembly. Within spliceosomes, disruption of base-paired U4/U6 heterodimer allows U6 snRNA to form part of the catalytic center [14]. Following completion of the splicing reaction, snRNPs must be recycled for subsequent rounds of splicing, although little is known about this process. Here we present evidence that regeneration of splicing activity in vitro is dependent on Lsm proteins. RNP reconstitution experiments with exogenous U6 RNA show that Lsm proteins promote the formation of U6-containing complexes and suggest that Lsm proteins have a chaperone-like function, supporting the assembly or remodeling of RNP complexes involved in splicing. Such a function could explain the involvement of Lsm proteins in a wide variety of RNA processing pathways. |
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| AbstractList | Lsm proteins are ubiquitous, multifunctional proteins that affect the processing of most RNAs in eukaryotic cells, but their function is unknown. A complex of seven Lsm proteins, Lsm2-8, associates with the U6 small nuclear RNA (snRNA) that is a component of spliceosome complexes in which pre-mRNA splicing occurs. Spliceosomes contain five snRNAs, U1, U2, U4, U5, and U6, that are packaged as ribonucleoprotein particles (snRNPs). U4 and U6 snRNAs contain extensive sequence complementarity and interact to form U4/U6 di-snRNPs. U4/U6 di-snRNPs associate with U5 snRNPs to form U4/U6.U5 tri-snRNPs prior to spliceosome assembly. Within spliceosomes, disruption of base-paired U4/U6 heterodimer allows U6 snRNA to form part of the catalytic center. Following completion of the splicing reaction, snRNPs must be recycled for subsequent rounds of splicing, although little is known about this process. Here we present evidence that regeneration of splicing activity in vitro is dependent on Lsm proteins. RNP reconstitution experiments with exogenous U6 RNA show that Lsm proteins promote the formation of U6-containing complexes and suggest that Lsm proteins have a chaperone-like function, supporting the assembly or remodeling of RNP complexes involved in splicing. Such a function could explain the involvement of Lsm proteins in a wide variety of RNA processing pathways. Lsm proteins are ubiquitous, multifunctional proteins that affect the processing of most RNAs in eukaryotic cells [1–11], but their function is unknown. A complex of seven Lsm proteins, Lsm2-8, associates with the U6 small nuclear RNA (snRNA) that is a component of spliceosome complexes in which pre-mRNA splicing occurs. Spliceosomes contain five snRNAs, U1, U2, U4, U5, and U6, that are packaged as ribonucleoprotein particles (snRNPs) [12, 13]. U4 and U6 snRNAs contain extensive sequence complementarity and interact to form U4/U6 di-snRNPs. U4/U6 di-snRNPs associate with U5 snRNPs to form U4/U6.U5 tri-snRNPs prior to spliceosome assembly. Within spliceosomes, disruption of base-paired U4/U6 heterodimer allows U6 snRNA to form part of the catalytic center [14]. Following completion of the splicing reaction, snRNPs must be recycled for subsequent rounds of splicing, although little is known about this process. Here we present evidence that regeneration of splicing activity in vitro is dependent on Lsm proteins. RNP reconstitution experiments with exogenous U6 RNA show that Lsm proteins promote the formation of U6-containing complexes and suggest that Lsm proteins have a chaperone-like function, supporting the assembly or remodeling of RNP complexes involved in splicing. Such a function could explain the involvement of Lsm proteins in a wide variety of RNA processing pathways. |
| Author | Verdone, Loredana Page, David Beggs, Jean D. Galardi, Silvia |
| Author_xml | – sequence: 1 givenname: Loredana surname: Verdone fullname: Verdone, Loredana – sequence: 2 givenname: Silvia surname: Galardi fullname: Galardi, Silvia – sequence: 3 givenname: David surname: Page fullname: Page, David – sequence: 4 givenname: Jean D. surname: Beggs fullname: Beggs, Jean D. email: jbeggs@ed.ac.uk |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/15324666$$D View this record in MEDLINE/PubMed |
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| Snippet | Lsm proteins are ubiquitous, multifunctional proteins that affect the processing of most RNAs in eukaryotic cells [1–11], but their function is unknown. A... Lsm proteins are ubiquitous, multifunctional proteins that affect the processing of most RNAs in eukaryotic cells, but their function is unknown. A complex of... |
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| SubjectTerms | Multigene Family - genetics Oligonucleotides Plasmids - genetics Precipitin Tests Ribonucleoproteins, Small Nuclear - genetics Ribonucleoproteins, Small Nuclear - metabolism RNA Splicing - physiology RNA, Messenger - metabolism RNA, Small Nuclear - metabolism Spliceosomes - physiology Yeasts |
| Title | Lsm Proteins Promote Regeneration of Pre-mRNA Splicing Activity |
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