Measurement of eight scalar and dipolar couplings for methine?methylene pairs in proteins and nucleic acids

• Published in: Journal of biomolecular NMR Vol. 31; no. 3; pp. 201 - 216
• Main Authors: Miclet, Emeric, Boisbouvier, J r me, Bax, Ad
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.03.2005
• Subjects: Acids; Anisotropy; Bacterial Proteins - chemistry; Base Sequence; Carbon Isotopes; Escherichia coli - chemistry; Experiments; Hydrocarbons; Methane - analogs & derivatives; Methane - chemistry; Nitrogen Isotopes; Nuclear Magnetic Resonance, Biomolecular; Nucleic Acid Conformation; Nucleic acids; Nucleic Acids - chemistry; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Proteins; Proteins - chemistry; Residues; RNA, Bacterial - chemistry; RNA, Ribosomal, 23S - chemistry; Streptococcus

A new 3D, spin-state-selective coherence transfer NMR experiment is described that yields accurate measurements for eight scalar or dipolar couplings within a spin system composed of a methylene adjacent to a methine group. Implementations of the experiment have been optimized for proteins and for n...