Molecular epidemiology of pneumococcal isolates from children in China
To investigate the molecular epidemiology of pneumococcal isolates in Chongqing, China. In this cross-sectional study, 51 invasive Streptococcus pneumoniae (S. pneumoniae) strains were from children with invasive pneumococcal disease (IPD) and 32 carriage strains from healthy children from January 2...
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Published in | Saudi medical journal Vol. 37; no. 4; pp. 403 - 413 |
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Format | Journal Article |
Language | English |
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Saudi Medical Journal
01.04.2016
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Abstract | To investigate the molecular epidemiology of pneumococcal isolates in Chongqing, China.
In this cross-sectional study, 51 invasive Streptococcus pneumoniae (S. pneumoniae) strains were from children with invasive pneumococcal disease (IPD) and 32 carriage strains from healthy children from January 2010 to December 2013 at the Children's Hospital of Chongqing Medical University, Chongqing, China. Multilocus sequence typing was used to identify the sequence types (STs). Capsular serotypes were determined by multiplex polymerase chain reaction. Drug susceptibility and resistance was determined by minimum inhibitory concentrations.
In this study, 11 serotypes were identified among the 83 S. pneumoniae clinical isolates tested. Prevalent serotypes were 19A (20.4%), 6A/B (20.4%), 19F (15.7%), 14 (14.5%), and 23F (10.8%). Serotype 19F was the most frequent carriage strain, and serotype 19A was the most frequent invasive strain. The ST983 was the most prevalent ST for carriage strains, and ST320 was the most prevalent ST for invasive strains. For gene analysis, psaA (99.5%) and piaA (98.6%) were present and much conserved in all pneumococci tested. The cps2A and pcsB genes were more frequent in invasive isolates than carriage strains. Antimicrobial resistance rates of invasive pneumococcal isolates to erythromycin, penicillin, meropenem, cefotaxime, and clindamycin were higher than the carriage isolates from children.
Our epidemiological evidence shows that 19A, 6A/B, 19F, 14, and 23F remain the most prevalent serotypes, which can be targeted by PCV13. Genotypes and drug resistance varied between carriage and invasive strains. The PsaA and PiaA may be good protein vaccine candidates. |
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AbstractList | [phrase omitted] To investigate the molecular epidemiology of pneumococcal isolates in Chongqing, China. In this cross-sectional study, 51 invasive Streptococcus pneumoniae (S. pneumoniae) strains were from children with invasive pneumococcal disease (IPD) and 32 carriage strains from healthy children from January 2010 to December 2013 at the Children's Hospital of Chongqing Medical University, Chongqing, China. Multilocus sequence typing was used to identify the sequence types (STs). Capsular serotypes were determined by multiplex polymerase chain reaction. Drug susceptibility and resistance was determined by minimum inhibitory concentrations. In this study, 11 serotypes were identified among the 83 S. pneumoniae clinical isolates tested. Prevalent serotypes were 19A (20.4%), 6A/B (20.4%), 19F (15.7%), 14 (14.5%), and 23F (10.8%). Serotype 19F was the most frequent carriage strain, and serotype 19A was the most frequent invasive strain. The ST983 was the most prevalent ST for carriage strains, and ST320 was the most prevalent ST for invasive strains. For gene analysis, psaA (99.5%) and piaA (98.6%) were present and much conserved in all pneumococci tested. The cps2A and pcsB genes were more frequent in invasive isolates than carriage strains. Antimicrobial resistance rates of invasive pneumococcal isolates to erythromycin, penicillin, meropenem, cefotaxime, and clindamycin were higher than the carriage isolates from children. Our epidemiological evidence shows that 19A, 6A/B, 19F, 14, and 23F remain the most prevalent serotypes, which can be targeted by PCV13. Genotypes and drug resistance varied between carriage and invasive strains. The PsaA and PiaA may be good protein vaccine candidates. Objectives: To investigate the molecular epidemiology of pneumococcal isolates in Chongqing, China. Methods: In this cross-sectional study, 51 invasive Streptococcus pneumoniae (S. pneumoniae) strains were from children with invasive pneumococcal disease (IPD) and 32 carriage strains from healthy children from January 2010 to December 2013 at the Children's Hospital of Chongqing Medical University, Chongqing, China. Multilocus sequence typing was used to identify the sequence types (STs). Capsular serotypes were determined by multiplex polymerase chain reaction. Drug susceptibility and resistance was determined by minimum inhibitory concentrations. Results: In this study, 11 serotypes were identified among the 83 S. pneumoniae clinical isolates tested. Prevalent serotypes were 19A (20.4%), 6A/B (20.4%), 19F (15.7%), 14 (14.5%), and 23F (10.8%). Serotype 19F was the most frequent carriage strain, and serotype 19A was the most frequent invasive strain. The ST983 was the most prevalent ST for carriage strains, and ST320 was the most prevalent ST for invasive strains. For gene analysis, psaA (99.5%) and piaA (98.6%) were present and much conserved in all pneumococci tested. The cps2A and pcsB genes were more frequent in invasive isolates than carriage strains. Antimicrobial resistance rates of invasive pneumococcal isolates to erythromycin, penicillin, meropenem, cefotaxime, and clindamycin were higher than the carriage isolates from children. Conclusion: Our epidemiological evidence shows that 19A, 6A/B, 19F, 14, and 23F remain the most prevalent serotypes, which can be targeted by PCV13. Genotypes and drug resistance varied between carriage and invasive strains. The PsaA and PiaA may be good protein vaccine candidates. [phrase omitted] OBJECTIVESTo investigate the molecular epidemiology of pneumococcal isolates in Chongqing, China.METHODSIn this cross-sectional study, 51 invasive Streptococcus pneumoniae (S. pneumoniae) strains were from children with invasive pneumococcal disease (IPD) and 32 carriage strains from healthy children from January 2010 to December 2013 at the Children's Hospital of Chongqing Medical University, Chongqing, China. Multilocus sequence typing was used to identify the sequence types (STs). Capsular serotypes were determined by multiplex polymerase chain reaction. Drug susceptibility and resistance was determined by minimum inhibitory concentrations.RESULTSIn this study, 11 serotypes were identified among the 83 S. pneumoniae clinical isolates tested. Prevalent serotypes were 19A (20.4%), 6A/B (20.4%), 19F (15.7%), 14 (14.5%), and 23F (10.8%). Serotype 19F was the most frequent carriage strain, and serotype 19A was the most frequent invasive strain. The ST983 was the most prevalent ST for carriage strains, and ST320 was the most prevalent ST for invasive strains. For gene analysis, psaA (99.5%) and piaA (98.6%) were present and much conserved in all pneumococci tested. The cps2A and pcsB genes were more frequent in invasive isolates than carriage strains. Antimicrobial resistance rates of invasive pneumococcal isolates to erythromycin, penicillin, meropenem, cefotaxime, and clindamycin were higher than the carriage isolates from children.CONCLUSIONOur epidemiological evidence shows that 19A, 6A/B, 19F, 14, and 23F remain the most prevalent serotypes, which can be targeted by PCV13. Genotypes and drug resistance varied between carriage and invasive strains. The PsaA and PiaA may be good protein vaccine candidates. |
Audience | Academic |
Author | Zhang, Xue-Mei Xu, Wen-Chun Kang, Li-Hua Liu, Meng-Juan Zhang, Qun Cui, Jing-Jing Wu, Kai-Feng |
AuthorAffiliation | From the Key Laboratory of Diagnostic Medicine designated by the Ministry of Education (Kang, Liu, Xu, Cui, Zhang X, Wu), College of Laboratory Medicine, Chongqing Medical University, the Medicine Laboratory (Zhang Q), Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, the Department of Laboratory Medicine (Wu), the Third Affiliated Hospital of Zunyi Medical College, Zunyi, China |
AuthorAffiliation_xml | – name: From the Key Laboratory of Diagnostic Medicine designated by the Ministry of Education (Kang, Liu, Xu, Cui, Zhang X, Wu), College of Laboratory Medicine, Chongqing Medical University, the Medicine Laboratory (Zhang Q), Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, the Department of Laboratory Medicine (Wu), the Third Affiliated Hospital of Zunyi Medical College, Zunyi, China |
Author_xml | – sequence: 1 givenname: Li-Hua surname: Kang fullname: Kang, Li-Hua email: 82864898@qq.com organization: Key Laboratory of Diagnostic Medicine designated by the Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing, China. E-mail. 82864898@qq.com – sequence: 2 givenname: Meng-Juan surname: Liu fullname: Liu, Meng-Juan – sequence: 3 givenname: Wen-Chun surname: Xu fullname: Xu, Wen-Chun – sequence: 4 givenname: Jing-Jing surname: Cui fullname: Cui, Jing-Jing – sequence: 5 givenname: Xue-Mei surname: Zhang fullname: Zhang, Xue-Mei – sequence: 6 givenname: Kai-Feng surname: Wu fullname: Wu, Kai-Feng – sequence: 7 givenname: Qun surname: Zhang fullname: Zhang, Qun |
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CitedBy_id | crossref_primary_10_1080_21645515_2018_1435224 crossref_primary_10_1038_s41598_019_40088_6 crossref_primary_10_1371_journal_ppat_1006339 crossref_primary_10_1186_s12887_019_1722_1 crossref_primary_10_3389_fcimb_2019_00258 crossref_primary_10_1080_14760584_2017_1360771 |
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Snippet | To investigate the molecular epidemiology of pneumococcal isolates in Chongqing, China.
In this cross-sectional study, 51 invasive Streptococcus pneumoniae (S.... Objectives: To investigate the molecular epidemiology of pneumococcal isolates in Chongqing, China. Methods: In this cross-sectional study, 51 invasive... [phrase omitted] OBJECTIVESTo investigate the molecular epidemiology of pneumococcal isolates in Chongqing, China.METHODSIn this cross-sectional study, 51 invasive... |
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SubjectTerms | Anti-Bacterial Agents - pharmacology Antibacterial agents Carrier State - epidemiology Case-Control Studies Child Child, Preschool Children China - epidemiology Cross-Sectional Studies Epidemiology Female Humans Infant Male Medical schools Microbial drug resistance Microbial Sensitivity Tests Original Pneumococcal Infections - epidemiology Pneumococcal Infections - microbiology Pneumonia Serotyping Streptococcus pneumoniae - classification Streptococcus pneumoniae - genetics Streptococcus pneumoniae - isolation & purification Virulence - genetics |
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Title | Molecular epidemiology of pneumococcal isolates from children in China |
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