Neutrophil Chemotaxis on Silicone and Polyurethane Surfaces

• Published in: The Journal of infectious diseases Vol. 180; no. 5; pp. 1603 - 1607
• Main Authors: Indorf, Amy S., Poate, Tim, Sherertz, Robert J.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.11.1999; University of Chicago Press; Oxford University Press
• Subjects: Biological and medical sciences; Blood; Catheterization - instrumentation; Catheters; Chemotaxis; Chemotaxis, Leukocyte; Complement activation; Glass; Humans; Infections; Leukocytes; Major Articles; Medical sciences; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Neutrophils; Neutrophils - physiology; Phagocytosis; Polymers; Polyurethanes; Silicones; Surface Properties; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Zymosan - pharmacology; Infection; Sample preparation; Risk factor; Catheter; Biocompatibility; Neutrophil; Chemotaxis; In vitro; Silicone elastomer; Polyurethane elastomer
• DOI: 10.1086/315081
• ISSN: 0022-1899

Silicone vascular catheters have a greater risk of infection and produce greater inflammation in vivo and greater complement activation in vitro than other vascular catheter polymer materials. This study investigated whether polymorphonuclear leukocyte (PMNL) chemotaxis under agarose on silicone surfaces is different than on polyurethane (PU). Glass slides were coated with silicone and PU by use of a constant-speed dipping apparatus. Chemotaxis (3 h) in response to (10−7 mL) FMLP, zymosan-activated serum, and fresh serum (100%) was greater on silicone than on PU (P < .05). Polyclonal antibody to C5a blocked >50% of the movement toward serum (P < .05). Serum in the PMNL well significantly decreased chemotaxis toward FMLP on silicone (P < .05) but not on PU. These findings suggest that excessive complement activation by silicone may interfere with chemotaxis, but further work is necessary to determine whether this is relevant to an increased risk of catheter-related infection.