Modeling Amino Acid Metabolism in Mammalian Cells-Toward the Development of a Model Library
Amino acids are necessary to mammalian cell cultures both for protein synthesis and as an energy source. In this study, we present an unstructured mathematical model describing (i) cell growth and death kinetics and (ii) metabolism of glucose and 19 amino acids for HEK‐293 and CHO IFN‐γ cell culture...
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| Published in | Biotechnology progress Vol. 23; no. 6; pp. 1261 - 1269 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
USA
American Chemical Society
01.11.2007
American Institute of Chemical Engineers |
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| Online Access | Get full text |
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| Abstract | Amino acids are necessary to mammalian cell cultures both for protein synthesis and as an energy source. In this study, we present an unstructured mathematical model describing (i) cell growth and death kinetics and (ii) metabolism of glucose and 19 amino acids for HEK‐293 and CHO IFN‐γ cell cultures. The proposed mathematical framework is in good agreement with experimental data for both cell lines. It accommodates the inclusion of expressions for other cellular activities, such as the production of recombinant viral vectors or proteins, and can be used as the basis for the development of a model library for mammalian cell cultures. |
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| AbstractList | Amino acids are necessary to mammalian cell cultures both for protein synthesis and as an energy source. In this study, we present an unstructured mathematical model describing (i) cell growth and death kinetics and (ii) metabolism of glucose and 19 amino acids for HEK‐293 and CHO IFN‐γ cell cultures. The proposed mathematical framework is in good agreement with experimental data for both cell lines. It accommodates the inclusion of expressions for other cellular activities, such as the production of recombinant viral vectors or proteins, and can be used as the basis for the development of a model library for mammalian cell cultures. Amino acids are necessary to mammalian cell cultures both for protein synthesis and as an energy source. In this study, we present an unstructured mathematical model describing (i) cell growth and death kinetics and (ii) metabolism of glucose and 19 amino acids for HEK-293 and CHO IFN-gamma cell cultures. The proposed mathematical framework is in good agreement with experimental data for both cell lines. It accommodates the inclusion of expressions for other cellular activities, such as the production of recombinant viral vectors or proteins, and can be used as the basis for the development of a model library for mammalian cell cultures. |
| Author | Yap, Miranda G. S. Pistikopoulos, Efstratios N. Mantalaris, Athanasios Kontoravdi, Cleo Wong, Danny Lam, Carolyn Lee, Yih Yean |
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| Cites_doi | 10.1016/0168-1656(89)90091-6 10.1016/S1369-703X(99)00041-8 10.1007/BF00369551 10.1021/bp950078x 10.1002/(SICI)1097-0290(19980705)59:1<90::AID-BIT12>3.0.CO;2-6 10.1021/bp9701269 10.1021/bp060032b 10.1002/(SICI)1097-0290(20000120)67:2<197::AID-BIT9>3.0.CO;2-W 10.1021/bp990004b 10.1007/BF00762376 10.1007/BF00167898 10.1002/bit.260380602 10.1002/bit.260370109 10.1007/BF00749616 10.1016/S1369-703X(99)00021-2 10.1016/0378-4754(96)83703-7 10.1007/BF00772191 10.1021/bp050028k 10.1021/bp9900357 10.1007/BF00389535 10.1016/S0168-1656(97)00172-7 10.1016/B978-0-7506-1103-9.50116-5 |
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| Keywords | Models Metabolism Modeling Aminoacid |
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| SubjectTerms | Amino Acids - metabolism Animals Biological and medical sciences Biotechnology CHO Cells Cricetinae Cricetulus Databases, Factual Fundamental and applied biological sciences. Psychology Glucose - metabolism Models, Theoretical |
| Title | Modeling Amino Acid Metabolism in Mammalian Cells-Toward the Development of a Model Library |
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