Hydroxyl radical-induced decline in motility and increase in lipid peroxidation and DNA modification in human sperm

• Published in: Biochemistry and molecular biology international Vol. 43; no. 2; p. 291
• Main Authors: Chen, C S, Chao, H T, Pan, R L, Wei, Y H
• Format: Journal Article
• Language: English
• Published: England 01.10.1997
• Subjects: Adenosine Diphosphate - pharmacology; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - metabolism; DNA - drug effects; Ferrous Compounds - pharmacology; Humans; Hydrogen Peroxide - metabolism; Hydroxyl Radical - pharmacology; Kinetics; Lipid Peroxidation - drug effects; Male; Malondialdehyde - metabolism; Sperm Motility - drug effects; Spermatozoa - drug effects; Spermatozoa - physiology; Xanthine - metabolism; Xanthine Oxidase - metabolism

We employed the xanthine-xanthine oxidase system to produce H2O2 or simply used commercially available H2O2 solution to investigate the effects of exogenous hydroxyl radicals on the motility characteristics and on lipid peroxidation and DNA modification of human sperm. The functional parameters of s...