Long Term Culture of Human Kidney Proximal Tubule Epithelial Cells Maintains Lineage Functions and Serves as an Ex vivo Model for Coronavirus Associated Kidney Injury

• Published in: Virologica Sinica Vol. 35; no. 3; pp. 311 - 320
• Main Authors: Xia, Siyu, Wu, Ming, Chen, Si, Zhang, Tao, Ye, Lina, Liu, Jun, Li, Hui
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.06.2020; KeAi Publishing Communications Ltd
• Subjects: ACE2; acute kidney injury; Angiotensin; Angiotensin-Converting Enzyme 2; Animal models; Animals; Betacoronavirus - metabolism; Betacoronavirus - pathogenicity; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell culture; Cell Culture Techniques - methods; Cell differentiation; Cell Line; cell lines; Coronavirus - metabolism; Coronavirus - pathogenicity; Coronavirus Infections - virology; Coronaviruses; COVID-19; COVID-19 infection; Disease Models, Animal; DNA Damage; DNA repair; drugs; Epithelial cells; Epithelial Cells - virology; epithelium; ex vivo studies; Humans; Immune response; Innate immunity; Kidney - virology; Kidneys; luciferase; Medical Microbiology; Microbial Genetics and Genomics; Microbiology; neoplasm cells; Oncology; Organoids; Pandemics; Peptidyl-dipeptidase A; Peptidyl-Dipeptidase A - metabolism; Physiology; Pneumonia, Viral - virology; Receptors, Cell Surface - metabolism; Renal cell carcinoma; renal tubules; Research Article; SARS Virus - metabolism; SARS Virus - pathogenicity; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Sodium-Phosphate Cotransporter Proteins, Type IIc - metabolism; Spike Glycoprotein, Coronavirus - metabolism; Tumor cell lines; Virology; SARS-CoVs; Organoids; Conditionally reprogrammed cells (CRCs); Kidney proximal tubule epithelial cells (KPTECs); Angiotensin-converting enzyme 2 (ACE2)

The mechanism of how SARS-CoV-2 causes severe multi-organ failure is largely unknown. Acute kidney injury (AKI) is one of the frequent organ damage in severe COVID-19 patients. Previous studies have shown that human renal tubule cells could be the potential host cells targeted by SARS-CoV-2. Traditi...