PRC2 direct transfer from G-quadruplex RNA to dsDNA has implications for RNA-binding chromatin modifiers

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 120; no. 23; p. e2220528120
• Main Authors: Hemphill, Wayne O, Fenske, Regan, Gooding, Anne R, Cech, Thomas R
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 06.06.2023
• Subjects: Binding; Biological Sciences; Cell differentiation; Chromatin; Chromatin - genetics; Deoxyribonucleic acid; Differentiation (biology); DNA; DNA - genetics; DNA - metabolism; Embryogenesis; Embryonic growth stage; Enzymes; Epigenetics; Gene expression; Histone methyltransferase; In vivo methods and tests; Kinetics; Ligands; Nucleic acids; Nucleosomes; Nucleosomes - genetics; Polycomb group proteins; Polycomb Repressive Complex 2 - genetics; Polycomb Repressive Complex 2 - metabolism; Protein Binding; Ribonucleic acid; RNA; RNA - genetics; RNA - metabolism; displacement; exchange; methyltransferase; polynucleotide; nucleosomes

The chromatin-modifying enzyme, Polycomb Repressive Complex 2 (PRC2), deposits the H3K27me3 epigenetic mark to negatively regulate expression at numerous target genes, and this activity has been implicated in embryonic development, cell differentiation, and various cancers. A biological role for RNA...